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Purification and characterization of a novel laccase from Cerrena sp. HYB07 with dye decolorizing ability.

Yang J, Lin Q, Ng TB, Ye X, Lin J - PLoS ONE (2014)

Bottom Line: Its gene and cDNA sequences were obtained.Putative cis-acting transcriptional response elements were identified in the promoter region.The high production yield and activity, robustness and dye decolorizing capacity make LacA and Cerrena sp.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China; National Engineering Laboratory for Enzyme Expression, Fuzhou, Fujian, China.

ABSTRACT
Laccases (EC 1.10.3.2) are a class of multi-copper oxidases with important industrial values. A basidiomycete strain Cerrena sp. HYB07 with high laccase yield was identified. After cultivation in the shaking flask for 4 days, a maximal activity of 210.8 U mL(-1) was attained. A 58.6-kDa laccase (LacA) with 7.2% carbohydrate and a specific activity of 1952.4 U mg(-1) was purified. 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) was the optimal substrate, with Km and kcat being 93.4 µM and 2468.0 s(-1), respectively. LacA was stable at 60°C, pH 5.0 and above, and in organic solvents. Metal ions Na+, K+, Ca2+, Mg2+, Mn2+, Zn2+ enhanced LacA activity, while Fe2+ and Li+ inhibited LacA activity. LacA decolorized structurally different dyes and a real textile effluent. Its gene and cDNA sequences were obtained. Putative cis-acting transcriptional response elements were identified in the promoter region. The high production yield and activity, robustness and dye decolorizing capacity make LacA and Cerrena sp. HYB07 potentially useful for industrial and environmental applications such as textile finishing and wastewater treatment.

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LacA-mediated decolorization of a real textile effluent.(A) Absorption spectra of the real textile effluent during LacA-mediated decolorization recorded every 24 h for 6 d. (B) Absorption spectra of the real textile effluent without LacA.
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pone-0110834-g004: LacA-mediated decolorization of a real textile effluent.(A) Absorption spectra of the real textile effluent during LacA-mediated decolorization recorded every 24 h for 6 d. (B) Absorption spectra of the real textile effluent without LacA.

Mentions: Decolorization of the real textile effluent by LacA predominantly occurred within the first 3 d, accompanied by flattening of the absorption spectrum (Fig. 4A). A negative control was conducted in parallel, and there was little decrease in the absorbance in the absence of LacA (Fig. 4B).


Purification and characterization of a novel laccase from Cerrena sp. HYB07 with dye decolorizing ability.

Yang J, Lin Q, Ng TB, Ye X, Lin J - PLoS ONE (2014)

LacA-mediated decolorization of a real textile effluent.(A) Absorption spectra of the real textile effluent during LacA-mediated decolorization recorded every 24 h for 6 d. (B) Absorption spectra of the real textile effluent without LacA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214704&req=5

pone-0110834-g004: LacA-mediated decolorization of a real textile effluent.(A) Absorption spectra of the real textile effluent during LacA-mediated decolorization recorded every 24 h for 6 d. (B) Absorption spectra of the real textile effluent without LacA.
Mentions: Decolorization of the real textile effluent by LacA predominantly occurred within the first 3 d, accompanied by flattening of the absorption spectrum (Fig. 4A). A negative control was conducted in parallel, and there was little decrease in the absorbance in the absence of LacA (Fig. 4B).

Bottom Line: Its gene and cDNA sequences were obtained.Putative cis-acting transcriptional response elements were identified in the promoter region.The high production yield and activity, robustness and dye decolorizing capacity make LacA and Cerrena sp.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Sciences and Technology, Fuzhou University, Fuzhou, Fujian, China; National Engineering Laboratory for Enzyme Expression, Fuzhou, Fujian, China.

ABSTRACT
Laccases (EC 1.10.3.2) are a class of multi-copper oxidases with important industrial values. A basidiomycete strain Cerrena sp. HYB07 with high laccase yield was identified. After cultivation in the shaking flask for 4 days, a maximal activity of 210.8 U mL(-1) was attained. A 58.6-kDa laccase (LacA) with 7.2% carbohydrate and a specific activity of 1952.4 U mg(-1) was purified. 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) was the optimal substrate, with Km and kcat being 93.4 µM and 2468.0 s(-1), respectively. LacA was stable at 60°C, pH 5.0 and above, and in organic solvents. Metal ions Na+, K+, Ca2+, Mg2+, Mn2+, Zn2+ enhanced LacA activity, while Fe2+ and Li+ inhibited LacA activity. LacA decolorized structurally different dyes and a real textile effluent. Its gene and cDNA sequences were obtained. Putative cis-acting transcriptional response elements were identified in the promoter region. The high production yield and activity, robustness and dye decolorizing capacity make LacA and Cerrena sp. HYB07 potentially useful for industrial and environmental applications such as textile finishing and wastewater treatment.

Show MeSH
Related in: MedlinePlus