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Separation of hepatic iron and fat by dual-source dual-energy computed tomography based on material decomposition: an animal study.

Ma J, Song ZQ, Yan FH - PLoS ONE (2014)

Bottom Line: Spearman's correlation and one-way analysis of variance (ANOVA) were performed, respectively, to analyze statistically the correlations with the histopathological results and differences among groups.Different groups showed significantly different iron enhancement values and VNC values (F = 25.308,p<0.001; F = 10.911, p<0.001, respectively).Among the groups, significant differences in iron enhancement values were only observed between the iron-present and iron-absent groups, and differences in VNC values were only observed between the fat-present and fat-absent groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Shanghai Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Department of Radiology, the 2nd Affiliated Hospital of Shi-Hezi University Medical School (Corps Hospital), Urumqi, Xinjiang Province, China.

ABSTRACT

Objective: To explore the feasibility of dual-source dual-energy computed tomography (DSDECT) for hepatic iron and fat separation in vivo.

Materials and methods: All of the procedures in this study were approved by the Research Animal Resource Center of Shanghai Ruijin Hospital. Sixty rats that underwent DECT scanning were divided into the normal group, fatty liver group, liver iron group, and coexisting liver iron and fat group, according to Prussian blue and HE staining. The data for each group were reconstructed and post-processed by an iron-specific, three-material decomposition algorithm. The iron enhancement value and the virtual non-iron contrast value, which indicated overloaded liver iron and residual liver tissue, respectively, were measured. Spearman's correlation and one-way analysis of variance (ANOVA) were performed, respectively, to analyze statistically the correlations with the histopathological results and differences among groups.

Results: The iron enhancement values were positively correlated with the iron pathology grading (r = 0.729, p<0.001). Virtual non-iron contrast (VNC) values were negatively correlated with the fat pathology grading (r = -0.642,p<0.0001). Different groups showed significantly different iron enhancement values and VNC values (F = 25.308,p<0.001; F = 10.911, p<0.001, respectively). Among the groups, significant differences in iron enhancement values were only observed between the iron-present and iron-absent groups, and differences in VNC values were only observed between the fat-present and fat-absent groups.

Conclusion: Separation of hepatic iron and fat by dual energy material decomposition in vivo was feasible, even when they coexisted.

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Related in: MedlinePlus

Examples showing mild and severe iron deposition; steatosis also coexisted in the liver.a–b) In the general view image, the degree of iron or steatosis in the liver could barely be determined from the gray-scale or CT value. c–d) On the VIC image, the existence and degree of iron are indicated in red colored dots and the iron enhancement value; the existence and degree of steatosis can be measured by the VNC value. For heavier iron deposition, more intense red colored dots and higher iron enhancement values were observed. More severe steatosis resulted in a smaller VNC value.
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pone-0110964-g003: Examples showing mild and severe iron deposition; steatosis also coexisted in the liver.a–b) In the general view image, the degree of iron or steatosis in the liver could barely be determined from the gray-scale or CT value. c–d) On the VIC image, the existence and degree of iron are indicated in red colored dots and the iron enhancement value; the existence and degree of steatosis can be measured by the VNC value. For heavier iron deposition, more intense red colored dots and higher iron enhancement values were observed. More severe steatosis resulted in a smaller VNC value.

Mentions: Iron enhancement values increased with increasing TIS (r = 0.729, p<0.001) (Figure 2a). The VNC values of the hepatic parenchyma decreased with increasing histological steatosis (r = −0.642, p<0.001) (Figure 2b). The iron distribution and concentration, represented by red dots, could be obviously visualized and measured on the VIC images (Figure 3).


Separation of hepatic iron and fat by dual-source dual-energy computed tomography based on material decomposition: an animal study.

Ma J, Song ZQ, Yan FH - PLoS ONE (2014)

Examples showing mild and severe iron deposition; steatosis also coexisted in the liver.a–b) In the general view image, the degree of iron or steatosis in the liver could barely be determined from the gray-scale or CT value. c–d) On the VIC image, the existence and degree of iron are indicated in red colored dots and the iron enhancement value; the existence and degree of steatosis can be measured by the VNC value. For heavier iron deposition, more intense red colored dots and higher iron enhancement values were observed. More severe steatosis resulted in a smaller VNC value.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214685&req=5

pone-0110964-g003: Examples showing mild and severe iron deposition; steatosis also coexisted in the liver.a–b) In the general view image, the degree of iron or steatosis in the liver could barely be determined from the gray-scale or CT value. c–d) On the VIC image, the existence and degree of iron are indicated in red colored dots and the iron enhancement value; the existence and degree of steatosis can be measured by the VNC value. For heavier iron deposition, more intense red colored dots and higher iron enhancement values were observed. More severe steatosis resulted in a smaller VNC value.
Mentions: Iron enhancement values increased with increasing TIS (r = 0.729, p<0.001) (Figure 2a). The VNC values of the hepatic parenchyma decreased with increasing histological steatosis (r = −0.642, p<0.001) (Figure 2b). The iron distribution and concentration, represented by red dots, could be obviously visualized and measured on the VIC images (Figure 3).

Bottom Line: Spearman's correlation and one-way analysis of variance (ANOVA) were performed, respectively, to analyze statistically the correlations with the histopathological results and differences among groups.Different groups showed significantly different iron enhancement values and VNC values (F = 25.308,p<0.001; F = 10.911, p<0.001, respectively).Among the groups, significant differences in iron enhancement values were only observed between the iron-present and iron-absent groups, and differences in VNC values were only observed between the fat-present and fat-absent groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Shanghai Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Department of Radiology, the 2nd Affiliated Hospital of Shi-Hezi University Medical School (Corps Hospital), Urumqi, Xinjiang Province, China.

ABSTRACT

Objective: To explore the feasibility of dual-source dual-energy computed tomography (DSDECT) for hepatic iron and fat separation in vivo.

Materials and methods: All of the procedures in this study were approved by the Research Animal Resource Center of Shanghai Ruijin Hospital. Sixty rats that underwent DECT scanning were divided into the normal group, fatty liver group, liver iron group, and coexisting liver iron and fat group, according to Prussian blue and HE staining. The data for each group were reconstructed and post-processed by an iron-specific, three-material decomposition algorithm. The iron enhancement value and the virtual non-iron contrast value, which indicated overloaded liver iron and residual liver tissue, respectively, were measured. Spearman's correlation and one-way analysis of variance (ANOVA) were performed, respectively, to analyze statistically the correlations with the histopathological results and differences among groups.

Results: The iron enhancement values were positively correlated with the iron pathology grading (r = 0.729, p<0.001). Virtual non-iron contrast (VNC) values were negatively correlated with the fat pathology grading (r = -0.642,p<0.0001). Different groups showed significantly different iron enhancement values and VNC values (F = 25.308,p<0.001; F = 10.911, p<0.001, respectively). Among the groups, significant differences in iron enhancement values were only observed between the iron-present and iron-absent groups, and differences in VNC values were only observed between the fat-present and fat-absent groups.

Conclusion: Separation of hepatic iron and fat by dual energy material decomposition in vivo was feasible, even when they coexisted.

Show MeSH
Related in: MedlinePlus