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Separation of hepatic iron and fat by dual-source dual-energy computed tomography based on material decomposition: an animal study.

Ma J, Song ZQ, Yan FH - PLoS ONE (2014)

Bottom Line: Spearman's correlation and one-way analysis of variance (ANOVA) were performed, respectively, to analyze statistically the correlations with the histopathological results and differences among groups.Different groups showed significantly different iron enhancement values and VNC values (F = 25.308,p<0.001; F = 10.911, p<0.001, respectively).Among the groups, significant differences in iron enhancement values were only observed between the iron-present and iron-absent groups, and differences in VNC values were only observed between the fat-present and fat-absent groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Shanghai Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Department of Radiology, the 2nd Affiliated Hospital of Shi-Hezi University Medical School (Corps Hospital), Urumqi, Xinjiang Province, China.

ABSTRACT

Objective: To explore the feasibility of dual-source dual-energy computed tomography (DSDECT) for hepatic iron and fat separation in vivo.

Materials and methods: All of the procedures in this study were approved by the Research Animal Resource Center of Shanghai Ruijin Hospital. Sixty rats that underwent DECT scanning were divided into the normal group, fatty liver group, liver iron group, and coexisting liver iron and fat group, according to Prussian blue and HE staining. The data for each group were reconstructed and post-processed by an iron-specific, three-material decomposition algorithm. The iron enhancement value and the virtual non-iron contrast value, which indicated overloaded liver iron and residual liver tissue, respectively, were measured. Spearman's correlation and one-way analysis of variance (ANOVA) were performed, respectively, to analyze statistically the correlations with the histopathological results and differences among groups.

Results: The iron enhancement values were positively correlated with the iron pathology grading (r = 0.729, p<0.001). Virtual non-iron contrast (VNC) values were negatively correlated with the fat pathology grading (r = -0.642,p<0.0001). Different groups showed significantly different iron enhancement values and VNC values (F = 25.308,p<0.001; F = 10.911, p<0.001, respectively). Among the groups, significant differences in iron enhancement values were only observed between the iron-present and iron-absent groups, and differences in VNC values were only observed between the fat-present and fat-absent groups.

Conclusion: Separation of hepatic iron and fat by dual energy material decomposition in vivo was feasible, even when they coexisted.

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Related in: MedlinePlus

The rats were grouped by their liver histological results.Normal group: 1a) No lipid droplets in hepatocytes in the field of vision with HE staining. 1b) Prussian blue staining; there was no blue stained material in the field of vision, indicating the absence of iron deposition. Fatty liver group: 1c) Many hepatocytes were affected by lipid droplets on HE staining. 1d) Prussian blue staining resulted in no blue stained material, indicating the absence of iron deposition. Coexisting group: 1e) Many hepatocytes were affected by lipid droplets with HE staining. 1f) There were blue stained dots with Prussian blue staining, indicating significant iron deposition in this rat. Liver iron group: 1g) No hepatocytes were affected by lipid droplets on HE staining. 1h) There are blue stained dots in Prussian blue staining, indicating significant iron deposition in this rat.
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pone-0110964-g001: The rats were grouped by their liver histological results.Normal group: 1a) No lipid droplets in hepatocytes in the field of vision with HE staining. 1b) Prussian blue staining; there was no blue stained material in the field of vision, indicating the absence of iron deposition. Fatty liver group: 1c) Many hepatocytes were affected by lipid droplets on HE staining. 1d) Prussian blue staining resulted in no blue stained material, indicating the absence of iron deposition. Coexisting group: 1e) Many hepatocytes were affected by lipid droplets with HE staining. 1f) There were blue stained dots with Prussian blue staining, indicating significant iron deposition in this rat. Liver iron group: 1g) No hepatocytes were affected by lipid droplets on HE staining. 1h) There are blue stained dots in Prussian blue staining, indicating significant iron deposition in this rat.

Mentions: The total liver iron grade scores of the 60 rats ranged from 5 to 57. Total steatosis measurements ranged from 1% to 90%. There were 5 rats in the normal group, 9 rats in the fatty liver group, 37 rats in the coexisting group and 9 rats in the liver iron group (Table 1). Rats in the normal group showed rare hepatic iron and steatosis (Figure 1a and 1b). In the fatty liver group, many hepatocytes with lipid droplets could be found with HE staining (Figure 1c), while the cells were negative for Perls' Prussian blue staining (Figure 1d). Both lipid droplets and blue dots were observed in the coexisting group (Figure 1e and 1f). In the liver iron group, the HE staining was negative (Figure 1g), but there were many blue dots with Perls' Prussian blue staining (Figure 1h).


Separation of hepatic iron and fat by dual-source dual-energy computed tomography based on material decomposition: an animal study.

Ma J, Song ZQ, Yan FH - PLoS ONE (2014)

The rats were grouped by their liver histological results.Normal group: 1a) No lipid droplets in hepatocytes in the field of vision with HE staining. 1b) Prussian blue staining; there was no blue stained material in the field of vision, indicating the absence of iron deposition. Fatty liver group: 1c) Many hepatocytes were affected by lipid droplets on HE staining. 1d) Prussian blue staining resulted in no blue stained material, indicating the absence of iron deposition. Coexisting group: 1e) Many hepatocytes were affected by lipid droplets with HE staining. 1f) There were blue stained dots with Prussian blue staining, indicating significant iron deposition in this rat. Liver iron group: 1g) No hepatocytes were affected by lipid droplets on HE staining. 1h) There are blue stained dots in Prussian blue staining, indicating significant iron deposition in this rat.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214685&req=5

pone-0110964-g001: The rats were grouped by their liver histological results.Normal group: 1a) No lipid droplets in hepatocytes in the field of vision with HE staining. 1b) Prussian blue staining; there was no blue stained material in the field of vision, indicating the absence of iron deposition. Fatty liver group: 1c) Many hepatocytes were affected by lipid droplets on HE staining. 1d) Prussian blue staining resulted in no blue stained material, indicating the absence of iron deposition. Coexisting group: 1e) Many hepatocytes were affected by lipid droplets with HE staining. 1f) There were blue stained dots with Prussian blue staining, indicating significant iron deposition in this rat. Liver iron group: 1g) No hepatocytes were affected by lipid droplets on HE staining. 1h) There are blue stained dots in Prussian blue staining, indicating significant iron deposition in this rat.
Mentions: The total liver iron grade scores of the 60 rats ranged from 5 to 57. Total steatosis measurements ranged from 1% to 90%. There were 5 rats in the normal group, 9 rats in the fatty liver group, 37 rats in the coexisting group and 9 rats in the liver iron group (Table 1). Rats in the normal group showed rare hepatic iron and steatosis (Figure 1a and 1b). In the fatty liver group, many hepatocytes with lipid droplets could be found with HE staining (Figure 1c), while the cells were negative for Perls' Prussian blue staining (Figure 1d). Both lipid droplets and blue dots were observed in the coexisting group (Figure 1e and 1f). In the liver iron group, the HE staining was negative (Figure 1g), but there were many blue dots with Perls' Prussian blue staining (Figure 1h).

Bottom Line: Spearman's correlation and one-way analysis of variance (ANOVA) were performed, respectively, to analyze statistically the correlations with the histopathological results and differences among groups.Different groups showed significantly different iron enhancement values and VNC values (F = 25.308,p<0.001; F = 10.911, p<0.001, respectively).Among the groups, significant differences in iron enhancement values were only observed between the iron-present and iron-absent groups, and differences in VNC values were only observed between the fat-present and fat-absent groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Shanghai Zhongshan Hospital, Shanghai Medical College, Fudan University, Shanghai, China; Department of Radiology, the 2nd Affiliated Hospital of Shi-Hezi University Medical School (Corps Hospital), Urumqi, Xinjiang Province, China.

ABSTRACT

Objective: To explore the feasibility of dual-source dual-energy computed tomography (DSDECT) for hepatic iron and fat separation in vivo.

Materials and methods: All of the procedures in this study were approved by the Research Animal Resource Center of Shanghai Ruijin Hospital. Sixty rats that underwent DECT scanning were divided into the normal group, fatty liver group, liver iron group, and coexisting liver iron and fat group, according to Prussian blue and HE staining. The data for each group were reconstructed and post-processed by an iron-specific, three-material decomposition algorithm. The iron enhancement value and the virtual non-iron contrast value, which indicated overloaded liver iron and residual liver tissue, respectively, were measured. Spearman's correlation and one-way analysis of variance (ANOVA) were performed, respectively, to analyze statistically the correlations with the histopathological results and differences among groups.

Results: The iron enhancement values were positively correlated with the iron pathology grading (r = 0.729, p<0.001). Virtual non-iron contrast (VNC) values were negatively correlated with the fat pathology grading (r = -0.642,p<0.0001). Different groups showed significantly different iron enhancement values and VNC values (F = 25.308,p<0.001; F = 10.911, p<0.001, respectively). Among the groups, significant differences in iron enhancement values were only observed between the iron-present and iron-absent groups, and differences in VNC values were only observed between the fat-present and fat-absent groups.

Conclusion: Separation of hepatic iron and fat by dual energy material decomposition in vivo was feasible, even when they coexisted.

Show MeSH
Related in: MedlinePlus