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Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus

Apoptosis in tumors transfected with rl-RVG, NDV or PBS. The results reveal that the number of apoptotic cells (arrows) and the apoptotic index were markedly higher in the rl-RVG and NDV groups than in the PBS group (P<0.01) and that those of the rl-RVG group were higher compared with the NDV group (P<0.05; magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
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f6-ol-08-06-2569: Apoptosis in tumors transfected with rl-RVG, NDV or PBS. The results reveal that the number of apoptotic cells (arrows) and the apoptotic index were markedly higher in the rl-RVG and NDV groups than in the PBS group (P<0.01) and that those of the rl-RVG group were higher compared with the NDV group (P<0.05; magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.

Mentions: A TUNEL assay was performed to detect apoptosis in the tumor cells. The results revealed that the number of apoptotic cells and the apoptotic index were markedly higher in the rl-RVG and NDV groups compared with the PBS group (P<0.01), with the rl-RVG group exhibiting a higher number and index compared with the NDV group (P<0.05) (Fig. 6; Table II).


Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

Apoptosis in tumors transfected with rl-RVG, NDV or PBS. The results reveal that the number of apoptotic cells (arrows) and the apoptotic index were markedly higher in the rl-RVG and NDV groups than in the PBS group (P<0.01) and that those of the rl-RVG group were higher compared with the NDV group (P<0.05; magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214503&req=5

f6-ol-08-06-2569: Apoptosis in tumors transfected with rl-RVG, NDV or PBS. The results reveal that the number of apoptotic cells (arrows) and the apoptotic index were markedly higher in the rl-RVG and NDV groups than in the PBS group (P<0.01) and that those of the rl-RVG group were higher compared with the NDV group (P<0.05; magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
Mentions: A TUNEL assay was performed to detect apoptosis in the tumor cells. The results revealed that the number of apoptotic cells and the apoptotic index were markedly higher in the rl-RVG and NDV groups compared with the PBS group (P<0.01), with the rl-RVG group exhibiting a higher number and index compared with the NDV group (P<0.05) (Fig. 6; Table II).

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus