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Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus

(A–C) The cytoplasm of cells from the rl-RVG group was positive for RVG expression, but the cytoplasm from the PBS and NDV groups was not. (D–F) The cytoplasm of cells from the rl-RVG and NDV groups was positive for NDV expression, and the expression in the rl-RVG group was stronger compared with the NDV group. The PBS group exhibited a negative result for NDV expression (magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
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f5-ol-08-06-2569: (A–C) The cytoplasm of cells from the rl-RVG group was positive for RVG expression, but the cytoplasm from the PBS and NDV groups was not. (D–F) The cytoplasm of cells from the rl-RVG and NDV groups was positive for NDV expression, and the expression in the rl-RVG group was stronger compared with the NDV group. The PBS group exhibited a negative result for NDV expression (magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.

Mentions: Immunohistochemistry with primary antibodies for NDV and RVG was used to confirm the expression of the NDV and RVG proteins in the tumor tissues. The results revealed that, in the rl-RVG group, RVG was expressed in the tumor cell cytoplasm, but there was no RVG expression in the NDV and PBS groups (Fig. 5A1–A3). The cytoplasm of the cells from the rl-RVG and NDV groups was positive for RVG expression, and the rl-RVG group exhibited stronger expression compared with the NDV group, while the PBS group was negative for RVG expression (Fig. 5B1–B3). These results indicated that the NDV proteins were expressed in the cytoplasm of the tumor cells in the rl-RVG and NDV groups and were expressed to a greater extent in the rl-RVG group. Additionally, there were notably more mucous lakes in the RVG group (Fig. 5).


Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

(A–C) The cytoplasm of cells from the rl-RVG group was positive for RVG expression, but the cytoplasm from the PBS and NDV groups was not. (D–F) The cytoplasm of cells from the rl-RVG and NDV groups was positive for NDV expression, and the expression in the rl-RVG group was stronger compared with the NDV group. The PBS group exhibited a negative result for NDV expression (magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214503&req=5

f5-ol-08-06-2569: (A–C) The cytoplasm of cells from the rl-RVG group was positive for RVG expression, but the cytoplasm from the PBS and NDV groups was not. (D–F) The cytoplasm of cells from the rl-RVG and NDV groups was positive for NDV expression, and the expression in the rl-RVG group was stronger compared with the NDV group. The PBS group exhibited a negative result for NDV expression (magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
Mentions: Immunohistochemistry with primary antibodies for NDV and RVG was used to confirm the expression of the NDV and RVG proteins in the tumor tissues. The results revealed that, in the rl-RVG group, RVG was expressed in the tumor cell cytoplasm, but there was no RVG expression in the NDV and PBS groups (Fig. 5A1–A3). The cytoplasm of the cells from the rl-RVG and NDV groups was positive for RVG expression, and the rl-RVG group exhibited stronger expression compared with the NDV group, while the PBS group was negative for RVG expression (Fig. 5B1–B3). These results indicated that the NDV proteins were expressed in the cytoplasm of the tumor cells in the rl-RVG and NDV groups and were expressed to a greater extent in the rl-RVG group. Additionally, there were notably more mucous lakes in the RVG group (Fig. 5).

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus