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Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus

Expression of the (A) NDV and (B) RVG genes in the tumor tissues. (1) rl-RVG group, GAPDH; (2) NDV group, GAPDH; (3) PBS group, GAPDH; (4) rl-RVG group; (5) NDV group; (6) PBS group; (M) Marker. The results display clear (A) NDV gene expression (~462 bp) and (B) RVG gene expression (~176 bp) following transfection with rl-RVG. NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
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f3-ol-08-06-2569: Expression of the (A) NDV and (B) RVG genes in the tumor tissues. (1) rl-RVG group, GAPDH; (2) NDV group, GAPDH; (3) PBS group, GAPDH; (4) rl-RVG group; (5) NDV group; (6) PBS group; (M) Marker. The results display clear (A) NDV gene expression (~462 bp) and (B) RVG gene expression (~176 bp) following transfection with rl-RVG. NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.

Mentions: RT-PCR was used to detect RVG and NDV gene expression in the tumor tissue. The results revealed clear RVG gene expression (~176 bp) following transfection with rl-RVG. In the PBS and NDV groups, the RVG was not expressed. The NDV gene (~462 bp) was expressed in the rl-RVG and NDV groups, but was not expressed in the PBS group (Fig. 3).


Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

Expression of the (A) NDV and (B) RVG genes in the tumor tissues. (1) rl-RVG group, GAPDH; (2) NDV group, GAPDH; (3) PBS group, GAPDH; (4) rl-RVG group; (5) NDV group; (6) PBS group; (M) Marker. The results display clear (A) NDV gene expression (~462 bp) and (B) RVG gene expression (~176 bp) following transfection with rl-RVG. NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214503&req=5

f3-ol-08-06-2569: Expression of the (A) NDV and (B) RVG genes in the tumor tissues. (1) rl-RVG group, GAPDH; (2) NDV group, GAPDH; (3) PBS group, GAPDH; (4) rl-RVG group; (5) NDV group; (6) PBS group; (M) Marker. The results display clear (A) NDV gene expression (~462 bp) and (B) RVG gene expression (~176 bp) following transfection with rl-RVG. NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
Mentions: RT-PCR was used to detect RVG and NDV gene expression in the tumor tissue. The results revealed clear RVG gene expression (~176 bp) following transfection with rl-RVG. In the PBS and NDV groups, the RVG was not expressed. The NDV gene (~462 bp) was expressed in the rl-RVG and NDV groups, but was not expressed in the PBS group (Fig. 3).

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus