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Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus

Hematoxylin and eosin-stained tissue sections from the (A–C) tumor, (D–F) lung and (G–I) spleen demonstrating histopathological changes following transfection. (A–C) A larger quantity of subcutaneous tumor necrosis (blue arrows) is present in the tumor tissue from the rl-RVG group. (D–F) A more severe inflammatory reaction occurred in the lung of the mice in the rl-RVG group. (G–I) A larger quantity of multinucleated giant cells (red arrows) are present in the spleen tissue from the rl-RVG group (magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
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f2-ol-08-06-2569: Hematoxylin and eosin-stained tissue sections from the (A–C) tumor, (D–F) lung and (G–I) spleen demonstrating histopathological changes following transfection. (A–C) A larger quantity of subcutaneous tumor necrosis (blue arrows) is present in the tumor tissue from the rl-RVG group. (D–F) A more severe inflammatory reaction occurred in the lung of the mice in the rl-RVG group. (G–I) A larger quantity of multinucleated giant cells (red arrows) are present in the spleen tissue from the rl-RVG group (magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.

Mentions: The spleen tissues exhibited a significant increase in size in the rl-RVG and NDV groups of mice compared with the PBS group, and the spleen tissues were larger in the rl-RVG group compared with those in the NDV group. In the spleen, rl-RVG transfection induced more aggregation of multinucleated giant cells (Fig. 2).


Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

Hematoxylin and eosin-stained tissue sections from the (A–C) tumor, (D–F) lung and (G–I) spleen demonstrating histopathological changes following transfection. (A–C) A larger quantity of subcutaneous tumor necrosis (blue arrows) is present in the tumor tissue from the rl-RVG group. (D–F) A more severe inflammatory reaction occurred in the lung of the mice in the rl-RVG group. (G–I) A larger quantity of multinucleated giant cells (red arrows) are present in the spleen tissue from the rl-RVG group (magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214503&req=5

f2-ol-08-06-2569: Hematoxylin and eosin-stained tissue sections from the (A–C) tumor, (D–F) lung and (G–I) spleen demonstrating histopathological changes following transfection. (A–C) A larger quantity of subcutaneous tumor necrosis (blue arrows) is present in the tumor tissue from the rl-RVG group. (D–F) A more severe inflammatory reaction occurred in the lung of the mice in the rl-RVG group. (G–I) A larger quantity of multinucleated giant cells (red arrows) are present in the spleen tissue from the rl-RVG group (magnification, ×200). NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
Mentions: The spleen tissues exhibited a significant increase in size in the rl-RVG and NDV groups of mice compared with the PBS group, and the spleen tissues were larger in the rl-RVG group compared with those in the NDV group. In the spleen, rl-RVG transfection induced more aggregation of multinucleated giant cells (Fig. 2).

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus