Limits...
Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus

Growth curves of the tumors present in the rl-RVG, NDV and PBS groups. Tumor inhibition rate of rl-RVG = (1960.92 − 804.96) / 1960.92 × 100 = 58.9%. Tumor inhibition rate of NDV = (1960.92–1127.88) / 1960.92× 100 = 42.5%. NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4214503&req=5

f1-ol-08-06-2569: Growth curves of the tumors present in the rl-RVG, NDV and PBS groups. Tumor inhibition rate of rl-RVG = (1960.92 − 804.96) / 1960.92 × 100 = 58.9%. Tumor inhibition rate of NDV = (1960.92–1127.88) / 1960.92× 100 = 42.5%. NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.

Mentions: The tumor volume in the PBS group continued to increase throughout the 21-day period. In the rl-RVG and NDV groups, the volumes were markedly smaller at day 21 compared with those detected in the PBS group (P<0.01), and the tumor volumes in the rl-RVG group were smaller compared with the NDV group (P<0.05). The tumor inhibition rates were calculated based on the following formula: Volume = a2 × b × 0.52. The tumor inhibition rate of rl-RVG was revealed to be 58.9%, which meant that the growth of the tumor in the rl-RVG group was inhibited by 58.9% compared with the PBS group. The tumor inhibition rate of NDV was 42.5%, meaning that the growth of the tumor in the NDV group was inhibited by 42.5% compared with the PBS group (Fig. 1; Table I).


Effect of recombinant Newcastle disease virus transfection on lung adenocarcinoma A549 cells in vivo.

Yan Y, Jia L, Zhang J, Liu Y, Bu X - Oncol Lett (2014)

Growth curves of the tumors present in the rl-RVG, NDV and PBS groups. Tumor inhibition rate of rl-RVG = (1960.92 − 804.96) / 1960.92 × 100 = 58.9%. Tumor inhibition rate of NDV = (1960.92–1127.88) / 1960.92× 100 = 42.5%. NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214503&req=5

f1-ol-08-06-2569: Growth curves of the tumors present in the rl-RVG, NDV and PBS groups. Tumor inhibition rate of rl-RVG = (1960.92 − 804.96) / 1960.92 × 100 = 58.9%. Tumor inhibition rate of NDV = (1960.92–1127.88) / 1960.92× 100 = 42.5%. NDV, Newcastle disease virus; RVG, rabies virus glycoprotein; rl-RVG, recombinant NDV; PBS, phosphate-buffered saline.
Mentions: The tumor volume in the PBS group continued to increase throughout the 21-day period. In the rl-RVG and NDV groups, the volumes were markedly smaller at day 21 compared with those detected in the PBS group (P<0.01), and the tumor volumes in the rl-RVG group were smaller compared with the NDV group (P<0.05). The tumor inhibition rates were calculated based on the following formula: Volume = a2 × b × 0.52. The tumor inhibition rate of rl-RVG was revealed to be 58.9%, which meant that the growth of the tumor in the rl-RVG group was inhibited by 58.9% compared with the PBS group. The tumor inhibition rate of NDV was 42.5%, meaning that the growth of the tumor in the NDV group was inhibited by 42.5% compared with the PBS group (Fig. 1; Table I).

Bottom Line: However, the effect of NDV on lung cancer has yet to be elucidated.Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group.The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Medicine, Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212002, P.R. China.

ABSTRACT
Newcastle disease virus (NDV) has been reported to selectively duplicate in and then destroy tumor cells, whilst sparing normal cells. However, the effect of NDV on lung cancer has yet to be elucidated. In the present study, recombinant NDV (rl-RVG) was applied to lung adenocarcinoma A549 cell tumor-bearing mice to explore its effect on the proliferation of the cells and the immune response of the mice. Following rl-RVG transfection, RVG and NDV gene expression, decreased tumor growth, subcutaneous tumor necrosis, tumor apoptosis and an increased number of cluster of differentiation (CD)3(-)/CD49(+) natural killer cells were more evident in the rl-RVG group. The present study demonstrated that rl-RVG transfection effectively restrained lung adenocarcinoma A549 cell growth in vivo, which may have been accomplish by inducing tumor cell apoptosis and regulating the cell immune response.

No MeSH data available.


Related in: MedlinePlus