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Overexpression of sorcin in multidrug-resistant human breast cancer.

Gong Z, Sun P, Chu H, Zhu H, Sun D, Chen J - Oncol Lett (2014)

Bottom Line: Its upregulation is usually associated with the development of chemotherapeutic drug resistance.The differential expression levels of sorcin were confirmed by western blot and immunohistochemical analysis.In conclusion, sorcin expression in the human serum of breast cancer patients who are resistant to NAC was elevated when compared with that of NAC-sensitive patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Sorcin is a soluble resistance-related calcium-binding protein, which is expressed in normal mammalian tissues, such as the liver, lungs and heart. It has been observed to be elevated in a number of cancer types, including colorectal, gastric and breast cancer. Its upregulation is usually associated with the development of chemotherapeutic drug resistance. The aim of this study was to evaluate the sorcin expression levels in human serum samples of breast cancer subjects at various stages, and subsequently compare the outcome of neoadjuvant chemotherapy when the sorcin levels fluctuated. In total, 50 subjects were recruited from patients who were admitted to Yantai Yuhunagding Hospital (Yantai, China) and diagnosed with breast cancer. Blood samples prior to and following chemotherapy were assessed using two-dimensional gel electrophoresis (2-DE) and western blot analysis. The 2-DE analysis of the serum samples revealed that sorcin was upregulated in six out of 29 neoadjuvant chemotherapy (NAC)-sensitive patients and, in those who developed multidrug resistance, sorcin was upregulated in 15 out of 21 patients (P<0.01). The differential expression levels of sorcin were confirmed by western blot and immunohistochemical analysis. In conclusion, sorcin expression in the human serum of breast cancer patients who are resistant to NAC was elevated when compared with that of NAC-sensitive patients.

No MeSH data available.


Related in: MedlinePlus

Validation of sorcin expression of infiltrating ductal breast cancer tissues by immunohistochemical staining. (A) Representative biopsy taken from patients who responded effectively to neoadjuvant chemotherapy, showing low sorcin expression. (B) Biopsy of the same patient following chemotherapy. (C) Representative biopsy taken from patients who did not respond to neoadjuavnt chemoptherapy, prior to chemotherapy. (D) Biopsy of the same patient following chemotherapy. Staining is evident in the cytoplasm, which is represented on the figure with dark brown color. Magnification ×200.
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f3-ol-08-06-2393: Validation of sorcin expression of infiltrating ductal breast cancer tissues by immunohistochemical staining. (A) Representative biopsy taken from patients who responded effectively to neoadjuvant chemotherapy, showing low sorcin expression. (B) Biopsy of the same patient following chemotherapy. (C) Representative biopsy taken from patients who did not respond to neoadjuavnt chemoptherapy, prior to chemotherapy. (D) Biopsy of the same patient following chemotherapy. Staining is evident in the cytoplasm, which is represented on the figure with dark brown color. Magnification ×200.

Mentions: Furthermore, as predicted, IHC of the infiltrating ductal breast cancerous tissue revealed heavy staining of sorcin in tissues obtained from patients with developed resistance to NAC. The results from two cases are shown in Fig. 3, where staining is particularly evident in the cytoplasm.


Overexpression of sorcin in multidrug-resistant human breast cancer.

Gong Z, Sun P, Chu H, Zhu H, Sun D, Chen J - Oncol Lett (2014)

Validation of sorcin expression of infiltrating ductal breast cancer tissues by immunohistochemical staining. (A) Representative biopsy taken from patients who responded effectively to neoadjuvant chemotherapy, showing low sorcin expression. (B) Biopsy of the same patient following chemotherapy. (C) Representative biopsy taken from patients who did not respond to neoadjuavnt chemoptherapy, prior to chemotherapy. (D) Biopsy of the same patient following chemotherapy. Staining is evident in the cytoplasm, which is represented on the figure with dark brown color. Magnification ×200.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214497&req=5

f3-ol-08-06-2393: Validation of sorcin expression of infiltrating ductal breast cancer tissues by immunohistochemical staining. (A) Representative biopsy taken from patients who responded effectively to neoadjuvant chemotherapy, showing low sorcin expression. (B) Biopsy of the same patient following chemotherapy. (C) Representative biopsy taken from patients who did not respond to neoadjuavnt chemoptherapy, prior to chemotherapy. (D) Biopsy of the same patient following chemotherapy. Staining is evident in the cytoplasm, which is represented on the figure with dark brown color. Magnification ×200.
Mentions: Furthermore, as predicted, IHC of the infiltrating ductal breast cancerous tissue revealed heavy staining of sorcin in tissues obtained from patients with developed resistance to NAC. The results from two cases are shown in Fig. 3, where staining is particularly evident in the cytoplasm.

Bottom Line: Its upregulation is usually associated with the development of chemotherapeutic drug resistance.The differential expression levels of sorcin were confirmed by western blot and immunohistochemical analysis.In conclusion, sorcin expression in the human serum of breast cancer patients who are resistant to NAC was elevated when compared with that of NAC-sensitive patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Sorcin is a soluble resistance-related calcium-binding protein, which is expressed in normal mammalian tissues, such as the liver, lungs and heart. It has been observed to be elevated in a number of cancer types, including colorectal, gastric and breast cancer. Its upregulation is usually associated with the development of chemotherapeutic drug resistance. The aim of this study was to evaluate the sorcin expression levels in human serum samples of breast cancer subjects at various stages, and subsequently compare the outcome of neoadjuvant chemotherapy when the sorcin levels fluctuated. In total, 50 subjects were recruited from patients who were admitted to Yantai Yuhunagding Hospital (Yantai, China) and diagnosed with breast cancer. Blood samples prior to and following chemotherapy were assessed using two-dimensional gel electrophoresis (2-DE) and western blot analysis. The 2-DE analysis of the serum samples revealed that sorcin was upregulated in six out of 29 neoadjuvant chemotherapy (NAC)-sensitive patients and, in those who developed multidrug resistance, sorcin was upregulated in 15 out of 21 patients (P<0.01). The differential expression levels of sorcin were confirmed by western blot and immunohistochemical analysis. In conclusion, sorcin expression in the human serum of breast cancer patients who are resistant to NAC was elevated when compared with that of NAC-sensitive patients.

No MeSH data available.


Related in: MedlinePlus