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Overexpression of sorcin in multidrug-resistant human breast cancer.

Gong Z, Sun P, Chu H, Zhu H, Sun D, Chen J - Oncol Lett (2014)

Bottom Line: Its upregulation is usually associated with the development of chemotherapeutic drug resistance.The differential expression levels of sorcin were confirmed by western blot and immunohistochemical analysis.In conclusion, sorcin expression in the human serum of breast cancer patients who are resistant to NAC was elevated when compared with that of NAC-sensitive patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Sorcin is a soluble resistance-related calcium-binding protein, which is expressed in normal mammalian tissues, such as the liver, lungs and heart. It has been observed to be elevated in a number of cancer types, including colorectal, gastric and breast cancer. Its upregulation is usually associated with the development of chemotherapeutic drug resistance. The aim of this study was to evaluate the sorcin expression levels in human serum samples of breast cancer subjects at various stages, and subsequently compare the outcome of neoadjuvant chemotherapy when the sorcin levels fluctuated. In total, 50 subjects were recruited from patients who were admitted to Yantai Yuhunagding Hospital (Yantai, China) and diagnosed with breast cancer. Blood samples prior to and following chemotherapy were assessed using two-dimensional gel electrophoresis (2-DE) and western blot analysis. The 2-DE analysis of the serum samples revealed that sorcin was upregulated in six out of 29 neoadjuvant chemotherapy (NAC)-sensitive patients and, in those who developed multidrug resistance, sorcin was upregulated in 15 out of 21 patients (P<0.01). The differential expression levels of sorcin were confirmed by western blot and immunohistochemical analysis. In conclusion, sorcin expression in the human serum of breast cancer patients who are resistant to NAC was elevated when compared with that of NAC-sensitive patients.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis of sorcin and β-actin expression in sample serum from breast cancer patients who were NAC-sensitive or who developed multidrug resistance, versus normal subjects. NAC, neoadjuvant chemotherapy; MDR, multidrug resistant.
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f2-ol-08-06-2393: Western blot analysis of sorcin and β-actin expression in sample serum from breast cancer patients who were NAC-sensitive or who developed multidrug resistance, versus normal subjects. NAC, neoadjuvant chemotherapy; MDR, multidrug resistant.

Mentions: Western blot analysis using anti-sorcin antibodies revealed a specific band in the sample pool of MDR serum, whereas no band was visible for the NAC-sensitive group (Fig. 2). Anti-β-actin antibodies were used as a control for the analysis.


Overexpression of sorcin in multidrug-resistant human breast cancer.

Gong Z, Sun P, Chu H, Zhu H, Sun D, Chen J - Oncol Lett (2014)

Western blot analysis of sorcin and β-actin expression in sample serum from breast cancer patients who were NAC-sensitive or who developed multidrug resistance, versus normal subjects. NAC, neoadjuvant chemotherapy; MDR, multidrug resistant.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214497&req=5

f2-ol-08-06-2393: Western blot analysis of sorcin and β-actin expression in sample serum from breast cancer patients who were NAC-sensitive or who developed multidrug resistance, versus normal subjects. NAC, neoadjuvant chemotherapy; MDR, multidrug resistant.
Mentions: Western blot analysis using anti-sorcin antibodies revealed a specific band in the sample pool of MDR serum, whereas no band was visible for the NAC-sensitive group (Fig. 2). Anti-β-actin antibodies were used as a control for the analysis.

Bottom Line: Its upregulation is usually associated with the development of chemotherapeutic drug resistance.The differential expression levels of sorcin were confirmed by western blot and immunohistochemical analysis.In conclusion, sorcin expression in the human serum of breast cancer patients who are resistant to NAC was elevated when compared with that of NAC-sensitive patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Sorcin is a soluble resistance-related calcium-binding protein, which is expressed in normal mammalian tissues, such as the liver, lungs and heart. It has been observed to be elevated in a number of cancer types, including colorectal, gastric and breast cancer. Its upregulation is usually associated with the development of chemotherapeutic drug resistance. The aim of this study was to evaluate the sorcin expression levels in human serum samples of breast cancer subjects at various stages, and subsequently compare the outcome of neoadjuvant chemotherapy when the sorcin levels fluctuated. In total, 50 subjects were recruited from patients who were admitted to Yantai Yuhunagding Hospital (Yantai, China) and diagnosed with breast cancer. Blood samples prior to and following chemotherapy were assessed using two-dimensional gel electrophoresis (2-DE) and western blot analysis. The 2-DE analysis of the serum samples revealed that sorcin was upregulated in six out of 29 neoadjuvant chemotherapy (NAC)-sensitive patients and, in those who developed multidrug resistance, sorcin was upregulated in 15 out of 21 patients (P<0.01). The differential expression levels of sorcin were confirmed by western blot and immunohistochemical analysis. In conclusion, sorcin expression in the human serum of breast cancer patients who are resistant to NAC was elevated when compared with that of NAC-sensitive patients.

No MeSH data available.


Related in: MedlinePlus