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Cantharidin induces G2/M phase arrest and apoptosis in human gastric cancer SGC-7901 and BGC-823 cells.

Zhang C, Chen Z, Zhou X, Xu W, Wang G, Tang X, Luo L, Tu J, Zhu Y, Hu W, Xu X, Pan W - Oncol Lett (2014)

Bottom Line: CTD also induced G2/M phase arrest and cellular apoptosis in a dose-dependent manner.In addition, CTD increased the levels of p21, caspase-7, -8 and -9, activated caspase-3, poly ADP ribose polymerase and Bad, but decreased the levels of cyclin-dependent kinase 1, cyclin A and B, B-cell lymphoma-2 (Bcl-2) and Bid.CTD may induce cellular G2/M phase arrest by regulating cycle-associated proteins and induce apoptosis by activating a caspase cascade or regulating the Bcl-2 family proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.

ABSTRACT
The aim of the present study was to investigate the effect of cantharidin (CTD) on human gastric cancer cells and to explore the underlying mechanisms of these effects. The human gastric cancer SGC-7901 and BGC-823 cell lines were treated with CTD. MTS assays were then employed to examine cellular proliferation, flow cytometry was used to analyze the cell cycle and apoptosis, and western blot analysis was used to determine protein expression levels. It was found that CTD inhibited the proliferation of the human gastric cancer SGC-7901 and BGC-823 cells in a dose- and time-dependent manner in vitro. CTD also induced G2/M phase arrest and cellular apoptosis in a dose-dependent manner. In addition, CTD increased the levels of p21, caspase-7, -8 and -9, activated caspase-3, poly ADP ribose polymerase and Bad, but decreased the levels of cyclin-dependent kinase 1, cyclin A and B, B-cell lymphoma-2 (Bcl-2) and Bid. The present results suggested that CTD may inhibit the proliferation of human gastric cancer SGC-7901 and BGC-823 cells in vitro by inducing G2/M phase arrest and cell apoptosis. CTD may induce cellular G2/M phase arrest by regulating cycle-associated proteins and induce apoptosis by activating a caspase cascade or regulating the Bcl-2 family proteins.

No MeSH data available.


Related in: MedlinePlus

Cantharidin (CTD) affects cell cycle distribution in (A and C) SGC-7901 and (B and D) BGC-823 cells. The cells were treated with a graded concentration (0, 5, 10 and 20 μM) of CTD for suitable periods of time with 24-h intervals. Flow cytometry was used to analyze cell cycle progression.
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f2-ol-08-06-2721: Cantharidin (CTD) affects cell cycle distribution in (A and C) SGC-7901 and (B and D) BGC-823 cells. The cells were treated with a graded concentration (0, 5, 10 and 20 μM) of CTD for suitable periods of time with 24-h intervals. Flow cytometry was used to analyze cell cycle progression.

Mentions: To investigate whether CTD induced inhibition of SGC-7901 and BGC-823 cell growth via cell cycle-arresting mechanisms, flow cytometry was used to analyze cell cycle distribution subsequent to the cells being treated with 0, 5, 10 and 20 μM CTD for 24 h. In the SGC-7901 cells treated with 0, 5, 10 or 20 μM CTD, the ratios of cells in the G2/M phase were 14.67, 20.71, 34.92 and 47.32%, respectively. The ratios for the BGC-823 cells treated with 0, 5, 10 and 20 μM of CTD were 4.31, 9.66, 26.12 and 34.97%, respectively. The results revealed that CTD increased the ratio of cells in the G2/M phase in the SGC-7901 and BGC-823 cells in a dose-dependent manner (Fig. 2).


Cantharidin induces G2/M phase arrest and apoptosis in human gastric cancer SGC-7901 and BGC-823 cells.

Zhang C, Chen Z, Zhou X, Xu W, Wang G, Tang X, Luo L, Tu J, Zhu Y, Hu W, Xu X, Pan W - Oncol Lett (2014)

Cantharidin (CTD) affects cell cycle distribution in (A and C) SGC-7901 and (B and D) BGC-823 cells. The cells were treated with a graded concentration (0, 5, 10 and 20 μM) of CTD for suitable periods of time with 24-h intervals. Flow cytometry was used to analyze cell cycle progression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214476&req=5

f2-ol-08-06-2721: Cantharidin (CTD) affects cell cycle distribution in (A and C) SGC-7901 and (B and D) BGC-823 cells. The cells were treated with a graded concentration (0, 5, 10 and 20 μM) of CTD for suitable periods of time with 24-h intervals. Flow cytometry was used to analyze cell cycle progression.
Mentions: To investigate whether CTD induced inhibition of SGC-7901 and BGC-823 cell growth via cell cycle-arresting mechanisms, flow cytometry was used to analyze cell cycle distribution subsequent to the cells being treated with 0, 5, 10 and 20 μM CTD for 24 h. In the SGC-7901 cells treated with 0, 5, 10 or 20 μM CTD, the ratios of cells in the G2/M phase were 14.67, 20.71, 34.92 and 47.32%, respectively. The ratios for the BGC-823 cells treated with 0, 5, 10 and 20 μM of CTD were 4.31, 9.66, 26.12 and 34.97%, respectively. The results revealed that CTD increased the ratio of cells in the G2/M phase in the SGC-7901 and BGC-823 cells in a dose-dependent manner (Fig. 2).

Bottom Line: CTD also induced G2/M phase arrest and cellular apoptosis in a dose-dependent manner.In addition, CTD increased the levels of p21, caspase-7, -8 and -9, activated caspase-3, poly ADP ribose polymerase and Bad, but decreased the levels of cyclin-dependent kinase 1, cyclin A and B, B-cell lymphoma-2 (Bcl-2) and Bid.CTD may induce cellular G2/M phase arrest by regulating cycle-associated proteins and induce apoptosis by activating a caspase cascade or regulating the Bcl-2 family proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.

ABSTRACT
The aim of the present study was to investigate the effect of cantharidin (CTD) on human gastric cancer cells and to explore the underlying mechanisms of these effects. The human gastric cancer SGC-7901 and BGC-823 cell lines were treated with CTD. MTS assays were then employed to examine cellular proliferation, flow cytometry was used to analyze the cell cycle and apoptosis, and western blot analysis was used to determine protein expression levels. It was found that CTD inhibited the proliferation of the human gastric cancer SGC-7901 and BGC-823 cells in a dose- and time-dependent manner in vitro. CTD also induced G2/M phase arrest and cellular apoptosis in a dose-dependent manner. In addition, CTD increased the levels of p21, caspase-7, -8 and -9, activated caspase-3, poly ADP ribose polymerase and Bad, but decreased the levels of cyclin-dependent kinase 1, cyclin A and B, B-cell lymphoma-2 (Bcl-2) and Bid. The present results suggested that CTD may inhibit the proliferation of human gastric cancer SGC-7901 and BGC-823 cells in vitro by inducing G2/M phase arrest and cell apoptosis. CTD may induce cellular G2/M phase arrest by regulating cycle-associated proteins and induce apoptosis by activating a caspase cascade or regulating the Bcl-2 family proteins.

No MeSH data available.


Related in: MedlinePlus