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Establishment of an orthotopic transplantation tumor model in nude mice using a drug-resistant human ovarian cancer cell line with a high expression of c-Kit.

Yi C, Zhang L, Li L, Liu X, Ling S, Zhang F, Liang W - Oncol Lett (2014)

Bottom Line: The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed.Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues.This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China.

ABSTRACT
The resistance of ovarian cancer to platinum-based chemotherapy is a critical issue in the clinical setting. The present study aimed to establish animal models to replicate this clinical condition, as well as to investigate the resistance mechanisms of ovarian cancer. A cisplatin (DDP)-resistant human ovarian cancer cell line, SKOV3/DDP, was screened, validated and injected subcutaneously into the neck of female nude mice. Following tumor establishment, the tumor was collected and cut into small sections, which were subsequently implanted into the ovaries of other nude mice. The growth of the orthotopic tumors was observed and the tumor-bearing mice were sacrificed and dissected. The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed. In the present study, 16 nude mice underwent orthotopic transplantation surgery and a tumor model was successfully established in 14/16 of the mice, with an in situ tumor formation rate of 87.5%. Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues. Therefore, the present study successfully established an orthotopic tumor transplantation model in nude mice using a c-Kit-positive DDP-resistant human ovarian cancer cell line. This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

No MeSH data available.


Related in: MedlinePlus

Microscopic images of (A) a pathological section of a healthy fallopian tube in a control mouse and (B and C) metastatic tumors in the bilateral fallopian tubes in an orthotopic transplantation nude mouse model. Hematoxylin and eosin stain; magnification, ×400.
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f6-ol-08-06-2611: Microscopic images of (A) a pathological section of a healthy fallopian tube in a control mouse and (B and C) metastatic tumors in the bilateral fallopian tubes in an orthotopic transplantation nude mouse model. Hematoxylin and eosin stain; magnification, ×400.

Mentions: The orthotopic tumors and organs with suspected metastatic tumors were resectioned, stained with H&E and analyzed under a microscope. A large number of ovarian cancer cells were observed in the bilateral ovaries and demonstrated infiltrative growth. The cell morphology was observed to be the same as that of the parental subcutaneous tumor, with large dark-stained nuclei and a high karyoplasmic ratio. The cells exhibited evident atypia, and a dense and disordered arrangement with visible interstitial cells (Fig. 5). Certain mice exhibited visible bilateral tubal metastasis of the tumor cells, with a morphology the same as that of the tumor cells that were transplanted orthotopically (Fig. 6). In the liver, the hepatic cord cells showed disarrangement, with evident inflammatory cell infiltration and small nodule formation. There were no metastatic tumor cells visible in the uterine tissue.


Establishment of an orthotopic transplantation tumor model in nude mice using a drug-resistant human ovarian cancer cell line with a high expression of c-Kit.

Yi C, Zhang L, Li L, Liu X, Ling S, Zhang F, Liang W - Oncol Lett (2014)

Microscopic images of (A) a pathological section of a healthy fallopian tube in a control mouse and (B and C) metastatic tumors in the bilateral fallopian tubes in an orthotopic transplantation nude mouse model. Hematoxylin and eosin stain; magnification, ×400.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214472&req=5

f6-ol-08-06-2611: Microscopic images of (A) a pathological section of a healthy fallopian tube in a control mouse and (B and C) metastatic tumors in the bilateral fallopian tubes in an orthotopic transplantation nude mouse model. Hematoxylin and eosin stain; magnification, ×400.
Mentions: The orthotopic tumors and organs with suspected metastatic tumors were resectioned, stained with H&E and analyzed under a microscope. A large number of ovarian cancer cells were observed in the bilateral ovaries and demonstrated infiltrative growth. The cell morphology was observed to be the same as that of the parental subcutaneous tumor, with large dark-stained nuclei and a high karyoplasmic ratio. The cells exhibited evident atypia, and a dense and disordered arrangement with visible interstitial cells (Fig. 5). Certain mice exhibited visible bilateral tubal metastasis of the tumor cells, with a morphology the same as that of the tumor cells that were transplanted orthotopically (Fig. 6). In the liver, the hepatic cord cells showed disarrangement, with evident inflammatory cell infiltration and small nodule formation. There were no metastatic tumor cells visible in the uterine tissue.

Bottom Line: The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed.Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues.This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China.

ABSTRACT
The resistance of ovarian cancer to platinum-based chemotherapy is a critical issue in the clinical setting. The present study aimed to establish animal models to replicate this clinical condition, as well as to investigate the resistance mechanisms of ovarian cancer. A cisplatin (DDP)-resistant human ovarian cancer cell line, SKOV3/DDP, was screened, validated and injected subcutaneously into the neck of female nude mice. Following tumor establishment, the tumor was collected and cut into small sections, which were subsequently implanted into the ovaries of other nude mice. The growth of the orthotopic tumors was observed and the tumor-bearing mice were sacrificed and dissected. The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed. In the present study, 16 nude mice underwent orthotopic transplantation surgery and a tumor model was successfully established in 14/16 of the mice, with an in situ tumor formation rate of 87.5%. Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues. Therefore, the present study successfully established an orthotopic tumor transplantation model in nude mice using a c-Kit-positive DDP-resistant human ovarian cancer cell line. This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

No MeSH data available.


Related in: MedlinePlus