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Establishment of an orthotopic transplantation tumor model in nude mice using a drug-resistant human ovarian cancer cell line with a high expression of c-Kit.

Yi C, Zhang L, Li L, Liu X, Ling S, Zhang F, Liang W - Oncol Lett (2014)

Bottom Line: The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed.Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues.This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China.

ABSTRACT
The resistance of ovarian cancer to platinum-based chemotherapy is a critical issue in the clinical setting. The present study aimed to establish animal models to replicate this clinical condition, as well as to investigate the resistance mechanisms of ovarian cancer. A cisplatin (DDP)-resistant human ovarian cancer cell line, SKOV3/DDP, was screened, validated and injected subcutaneously into the neck of female nude mice. Following tumor establishment, the tumor was collected and cut into small sections, which were subsequently implanted into the ovaries of other nude mice. The growth of the orthotopic tumors was observed and the tumor-bearing mice were sacrificed and dissected. The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed. In the present study, 16 nude mice underwent orthotopic transplantation surgery and a tumor model was successfully established in 14/16 of the mice, with an in situ tumor formation rate of 87.5%. Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues. Therefore, the present study successfully established an orthotopic tumor transplantation model in nude mice using a c-Kit-positive DDP-resistant human ovarian cancer cell line. This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

No MeSH data available.


Related in: MedlinePlus

Anatomic images showing ovarian cancer metastases to the (A) liver and (B) mesentery in an orthotopic transplantation nude mouse model.
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f4-ol-08-06-2611: Anatomic images showing ovarian cancer metastases to the (A) liver and (B) mesentery in an orthotopic transplantation nude mouse model.

Mentions: Abdominal swellings gradually appeared in the nude mice ~8–10 weeks after the successful orthotopic tumor transplantation. In addition, the mice exhibited loss of body weight and appetite, accompanied by decreased activity levels. Abdominal palpation revealed a palpable mass with poor mobility. The tumor-bearing mice were sacrificed via cervical dislocation when the abdominal mass was >2 cm in diameter. A laparotomy identified the formation of a relatively large mass in the transplantation side (Fig. 3), with a hard texture and varying degrees of adhesion to the surrounding tissues. A small quantity of dark-red intraperitoneal fluid was also observed. In certain mice, metastatic lesions were found in the abdominal organs, including the liver and mesentery (Fig. 4).


Establishment of an orthotopic transplantation tumor model in nude mice using a drug-resistant human ovarian cancer cell line with a high expression of c-Kit.

Yi C, Zhang L, Li L, Liu X, Ling S, Zhang F, Liang W - Oncol Lett (2014)

Anatomic images showing ovarian cancer metastases to the (A) liver and (B) mesentery in an orthotopic transplantation nude mouse model.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214472&req=5

f4-ol-08-06-2611: Anatomic images showing ovarian cancer metastases to the (A) liver and (B) mesentery in an orthotopic transplantation nude mouse model.
Mentions: Abdominal swellings gradually appeared in the nude mice ~8–10 weeks after the successful orthotopic tumor transplantation. In addition, the mice exhibited loss of body weight and appetite, accompanied by decreased activity levels. Abdominal palpation revealed a palpable mass with poor mobility. The tumor-bearing mice were sacrificed via cervical dislocation when the abdominal mass was >2 cm in diameter. A laparotomy identified the formation of a relatively large mass in the transplantation side (Fig. 3), with a hard texture and varying degrees of adhesion to the surrounding tissues. A small quantity of dark-red intraperitoneal fluid was also observed. In certain mice, metastatic lesions were found in the abdominal organs, including the liver and mesentery (Fig. 4).

Bottom Line: The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed.Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues.This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China.

ABSTRACT
The resistance of ovarian cancer to platinum-based chemotherapy is a critical issue in the clinical setting. The present study aimed to establish animal models to replicate this clinical condition, as well as to investigate the resistance mechanisms of ovarian cancer. A cisplatin (DDP)-resistant human ovarian cancer cell line, SKOV3/DDP, was screened, validated and injected subcutaneously into the neck of female nude mice. Following tumor establishment, the tumor was collected and cut into small sections, which were subsequently implanted into the ovaries of other nude mice. The growth of the orthotopic tumors was observed and the tumor-bearing mice were sacrificed and dissected. The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed. In the present study, 16 nude mice underwent orthotopic transplantation surgery and a tumor model was successfully established in 14/16 of the mice, with an in situ tumor formation rate of 87.5%. Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues. Therefore, the present study successfully established an orthotopic tumor transplantation model in nude mice using a c-Kit-positive DDP-resistant human ovarian cancer cell line. This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

No MeSH data available.


Related in: MedlinePlus