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Establishment of an orthotopic transplantation tumor model in nude mice using a drug-resistant human ovarian cancer cell line with a high expression of c-Kit.

Yi C, Zhang L, Li L, Liu X, Ling S, Zhang F, Liang W - Oncol Lett (2014)

Bottom Line: The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed.Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues.This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China.

ABSTRACT
The resistance of ovarian cancer to platinum-based chemotherapy is a critical issue in the clinical setting. The present study aimed to establish animal models to replicate this clinical condition, as well as to investigate the resistance mechanisms of ovarian cancer. A cisplatin (DDP)-resistant human ovarian cancer cell line, SKOV3/DDP, was screened, validated and injected subcutaneously into the neck of female nude mice. Following tumor establishment, the tumor was collected and cut into small sections, which were subsequently implanted into the ovaries of other nude mice. The growth of the orthotopic tumors was observed and the tumor-bearing mice were sacrificed and dissected. The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed. In the present study, 16 nude mice underwent orthotopic transplantation surgery and a tumor model was successfully established in 14/16 of the mice, with an in situ tumor formation rate of 87.5%. Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues. Therefore, the present study successfully established an orthotopic tumor transplantation model in nude mice using a c-Kit-positive DDP-resistant human ovarian cancer cell line. This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

No MeSH data available.


Related in: MedlinePlus

qPCR analysis of c-Kit mRNA expression in SKOV3 and SKOV3/DDP cells. qPCR, quantitative polymerase chain reaction analysis; DDP, cisplatin. **P<0.01 vs. SKOV3 group.
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f2-ol-08-06-2611: qPCR analysis of c-Kit mRNA expression in SKOV3 and SKOV3/DDP cells. qPCR, quantitative polymerase chain reaction analysis; DDP, cisplatin. **P<0.01 vs. SKOV3 group.

Mentions: The dose- and time-dependent cytotoxic effects of DDP on the cultured SKOV3 and SKOV3/DDP cells were determined using an MTT colorimetric method. Cell growth and survival curves were then plotted (Fig. 1). As shown in Fig. 1, DDP resistance was significantly higher in the SKOV3/DDP cells compared with the SKOV3 cells following treatment with DDP for 24, 48 and 72 h. Furthermore, qPCR analysis (Fig. 2) revealed that c-Kit mRNA was expressed in the SKOV3 and SKOV3/DDP cells, and that the expression of c-Kit in the DDP-resistant SKOV3/DDP cells was significantly higher compared with that in the non-resistant SKOV3 cells.


Establishment of an orthotopic transplantation tumor model in nude mice using a drug-resistant human ovarian cancer cell line with a high expression of c-Kit.

Yi C, Zhang L, Li L, Liu X, Ling S, Zhang F, Liang W - Oncol Lett (2014)

qPCR analysis of c-Kit mRNA expression in SKOV3 and SKOV3/DDP cells. qPCR, quantitative polymerase chain reaction analysis; DDP, cisplatin. **P<0.01 vs. SKOV3 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214472&req=5

f2-ol-08-06-2611: qPCR analysis of c-Kit mRNA expression in SKOV3 and SKOV3/DDP cells. qPCR, quantitative polymerase chain reaction analysis; DDP, cisplatin. **P<0.01 vs. SKOV3 group.
Mentions: The dose- and time-dependent cytotoxic effects of DDP on the cultured SKOV3 and SKOV3/DDP cells were determined using an MTT colorimetric method. Cell growth and survival curves were then plotted (Fig. 1). As shown in Fig. 1, DDP resistance was significantly higher in the SKOV3/DDP cells compared with the SKOV3 cells following treatment with DDP for 24, 48 and 72 h. Furthermore, qPCR analysis (Fig. 2) revealed that c-Kit mRNA was expressed in the SKOV3 and SKOV3/DDP cells, and that the expression of c-Kit in the DDP-resistant SKOV3/DDP cells was significantly higher compared with that in the non-resistant SKOV3 cells.

Bottom Line: The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed.Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues.This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, The First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China.

ABSTRACT
The resistance of ovarian cancer to platinum-based chemotherapy is a critical issue in the clinical setting. The present study aimed to establish animal models to replicate this clinical condition, as well as to investigate the resistance mechanisms of ovarian cancer. A cisplatin (DDP)-resistant human ovarian cancer cell line, SKOV3/DDP, was screened, validated and injected subcutaneously into the neck of female nude mice. Following tumor establishment, the tumor was collected and cut into small sections, which were subsequently implanted into the ovaries of other nude mice. The growth of the orthotopic tumors was observed and the tumor-bearing mice were sacrificed and dissected. The orthotopic and metastatic tumor tissues were collected, sectioned, stained with hematoxylin and eosin and analyzed. In the present study, 16 nude mice underwent orthotopic transplantation surgery and a tumor model was successfully established in 14/16 of the mice, with an in situ tumor formation rate of 87.5%. Following euthanasia, a laparotomy demonstrated the tumor formation at the site of transplantation, as well as varying degrees of metastasis to additional organs and tissues. Therefore, the present study successfully established an orthotopic tumor transplantation model in nude mice using a c-Kit-positive DDP-resistant human ovarian cancer cell line. This model may represent a useful tool for investigating the resistance mechanism of ovarian cancer, as well as evaluating the efficacy of therapeutic strategies.

No MeSH data available.


Related in: MedlinePlus