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Effects of co-treatment with sulforaphane and autophagy modulators on uridine 5'-diphospho-glucuronosyltransferase 1A isoforms and cytochrome P450 3A4 expression in Caco-2 human colon cancer cells.

Wang M, Zhu JY, Chen S, Qing Y, Wu D, Lin YM, Luo JZ, Han W, Li YQ - Oncol Lett (2014)

Bottom Line: Sulforaphane (SFN), which is highly enriched in cruciferous vegetables, has been investigated for its cancer chemopreventive properties and ability to induce autophagy.The current study demonstrates that rapamycin may enhance the chemopreventive effects of SFN on Caco-2 cells; this may be partially attributed to nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2)- and human pregnane X receptor (hPXR)-mediated UGT1A1, UGT1A8 and UGT1A10 induction.These results indicate that targeting autophagy modulation may be a promising strategy for increasing the chemopreventive effects of SFN in cases of colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics and Gastroenterology, Qi-Lu Hospital of Shandong University, Key Laboratory of Proteomics of Shandong, Jinan, Shandong 250012, P.R. China.

ABSTRACT
Sulforaphane (SFN), which is highly enriched in cruciferous vegetables, has been investigated for its cancer chemopreventive properties and ability to induce autophagy. Uridine 5'-diphospho (UDP)-glucuronosyltransferase (UGT)1A induction is one of the mechanisms that is responsible for the cancer chemopreventive activity of SFN. The current study demonstrates that rapamycin may enhance the chemopreventive effects of SFN on Caco-2 cells; this may be partially attributed to nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2)- and human pregnane X receptor (hPXR)-mediated UGT1A1, UGT1A8 and UGT1A10 induction. These results indicate that targeting autophagy modulation may be a promising strategy for increasing the chemopreventive effects of SFN in cases of colon cancer.

No MeSH data available.


Related in: MedlinePlus

Quantitative reverse transcription polymerase chain reaction analysis of UGT1A1, UGT1A8 and UGT1A10 at the mRNA level in Caco-2 cells. Cells were treated for 24 h with 0 μM SFN (dimethyl sulfoxide control), 25 μM SFN, 10 nM rapamycin or 10 nM rapamycin plus 25 μM SFN. Results are presented as the mean ± standard error. n=3; *P<0.05 and **P<0.01. Ctr, control; SFN, sulforaphane.
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f5-ol-08-06-2407: Quantitative reverse transcription polymerase chain reaction analysis of UGT1A1, UGT1A8 and UGT1A10 at the mRNA level in Caco-2 cells. Cells were treated for 24 h with 0 μM SFN (dimethyl sulfoxide control), 25 μM SFN, 10 nM rapamycin or 10 nM rapamycin plus 25 μM SFN. Results are presented as the mean ± standard error. n=3; *P<0.05 and **P<0.01. Ctr, control; SFN, sulforaphane.

Mentions: To further investigate the modulation of rapamycin on UGT1A isoform expression by SFN, the mRNA levels of the UGT1A isoforms for the SFN-, rapamycin- and SFN/rapamycin-treated cells were analyzed using RT-qPCR. As shown in Fig. 5, similar trends were identified in the induction of UGT1A isoforms, when Caco-2 cells were treated with these small molecules. SFN-induced mRNA expression of UGT1A1, UGT1A8 and UGT1A10 was further enhanced in the presence of rapamycin (P=0.001, P=0.002 and P=0.003, respectively).


Effects of co-treatment with sulforaphane and autophagy modulators on uridine 5'-diphospho-glucuronosyltransferase 1A isoforms and cytochrome P450 3A4 expression in Caco-2 human colon cancer cells.

Wang M, Zhu JY, Chen S, Qing Y, Wu D, Lin YM, Luo JZ, Han W, Li YQ - Oncol Lett (2014)

Quantitative reverse transcription polymerase chain reaction analysis of UGT1A1, UGT1A8 and UGT1A10 at the mRNA level in Caco-2 cells. Cells were treated for 24 h with 0 μM SFN (dimethyl sulfoxide control), 25 μM SFN, 10 nM rapamycin or 10 nM rapamycin plus 25 μM SFN. Results are presented as the mean ± standard error. n=3; *P<0.05 and **P<0.01. Ctr, control; SFN, sulforaphane.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214451&req=5

f5-ol-08-06-2407: Quantitative reverse transcription polymerase chain reaction analysis of UGT1A1, UGT1A8 and UGT1A10 at the mRNA level in Caco-2 cells. Cells were treated for 24 h with 0 μM SFN (dimethyl sulfoxide control), 25 μM SFN, 10 nM rapamycin or 10 nM rapamycin plus 25 μM SFN. Results are presented as the mean ± standard error. n=3; *P<0.05 and **P<0.01. Ctr, control; SFN, sulforaphane.
Mentions: To further investigate the modulation of rapamycin on UGT1A isoform expression by SFN, the mRNA levels of the UGT1A isoforms for the SFN-, rapamycin- and SFN/rapamycin-treated cells were analyzed using RT-qPCR. As shown in Fig. 5, similar trends were identified in the induction of UGT1A isoforms, when Caco-2 cells were treated with these small molecules. SFN-induced mRNA expression of UGT1A1, UGT1A8 and UGT1A10 was further enhanced in the presence of rapamycin (P=0.001, P=0.002 and P=0.003, respectively).

Bottom Line: Sulforaphane (SFN), which is highly enriched in cruciferous vegetables, has been investigated for its cancer chemopreventive properties and ability to induce autophagy.The current study demonstrates that rapamycin may enhance the chemopreventive effects of SFN on Caco-2 cells; this may be partially attributed to nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2)- and human pregnane X receptor (hPXR)-mediated UGT1A1, UGT1A8 and UGT1A10 induction.These results indicate that targeting autophagy modulation may be a promising strategy for increasing the chemopreventive effects of SFN in cases of colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Geriatrics and Gastroenterology, Qi-Lu Hospital of Shandong University, Key Laboratory of Proteomics of Shandong, Jinan, Shandong 250012, P.R. China.

ABSTRACT
Sulforaphane (SFN), which is highly enriched in cruciferous vegetables, has been investigated for its cancer chemopreventive properties and ability to induce autophagy. Uridine 5'-diphospho (UDP)-glucuronosyltransferase (UGT)1A induction is one of the mechanisms that is responsible for the cancer chemopreventive activity of SFN. The current study demonstrates that rapamycin may enhance the chemopreventive effects of SFN on Caco-2 cells; this may be partially attributed to nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2)- and human pregnane X receptor (hPXR)-mediated UGT1A1, UGT1A8 and UGT1A10 induction. These results indicate that targeting autophagy modulation may be a promising strategy for increasing the chemopreventive effects of SFN in cases of colon cancer.

No MeSH data available.


Related in: MedlinePlus