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Hepatoma upregulated protein expression is involved in the pathogenesis of human breast carcinogenesis.

Chen J, Liu QJ, Wang D, Zhou XY, Xiong D, Li HJ, Li CL - Oncol Lett (2014)

Bottom Line: In the last decade, the overexpression of hepatoma upregulated protein (HURP) has been reported in hepatocellular carcinoma, adrenocortical tumors and urogenital carcinoma.The correlation between the HURP expression level and the clinicopathological characteristics was evaluated.The HURP expression level was higher in the tumors with advanced-grade metastasis and was strongly associated with tumor-node-metastasis staging (P=0.003).

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Sichuan University, Chengdu, Sichuan 610072, P.R. China.

ABSTRACT
In the last decade, the overexpression of hepatoma upregulated protein (HURP) has been reported in hepatocellular carcinoma, adrenocortical tumors and urogenital carcinoma. However, the role of HURP in breast cancer remains unknown. In the present study, a comprehensive analysis was performed to examine the HURP expression level in 43 breast cancer tumor samples and paired adjacent normal tissues. The correlation between the HURP expression level and the clinicopathological characteristics was evaluated. The role of HURP in breast cancer was investigated by quantitative polymerase chain reaction, western blot analysis and cell proliferation assays. HURP expression was found to be significantly increased in the breast cancer samples. The HURP expression level was higher in the tumors with advanced-grade metastasis and was strongly associated with tumor-node-metastasis staging (P=0.003). Transfection and cell proliferation assays suggested that the suppression of HURP expression or the interference in HURP activity in the breast cancer cells inhibited cell proliferation significantly. These data suggest that HURP is associated with the degree of malignancy and the proliferation of breast cancer. HURP could be a tumor biomarker for prognosis and a potential therapeutic drug target for human breast cancer.

No MeSH data available.


Related in: MedlinePlus

Interference in hepatoma upregulated protein (HURP) expression inhibits breast cancer cell proliferation in vitro. (A) MDA-MB-231 cells transfected with 2μg β-galactosidase (β-gal) using Xfect Protein Transfection Reagent. At 1 h post-transfection, the cells were assayed for β-gal activity using a β-Galactosidase Staining kit. The image was captured using an inverted microscope with ×100 magnification. The Xfect Protein Transfection Reagent displayed a markedly higher signal for β-gal. (B) MDA-MB-231 cell viability was measured using the Cell Counting Kit-8 assay for four days. The viability of the anti-HURP Ab-transfected cells was lower than that of the parent cells. The cells transfected with 5μg anti-HURP Ab grew slower than the other transfected cells (*P<0.05 and **P<0.01).
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f3-ol-08-06-2543: Interference in hepatoma upregulated protein (HURP) expression inhibits breast cancer cell proliferation in vitro. (A) MDA-MB-231 cells transfected with 2μg β-galactosidase (β-gal) using Xfect Protein Transfection Reagent. At 1 h post-transfection, the cells were assayed for β-gal activity using a β-Galactosidase Staining kit. The image was captured using an inverted microscope with ×100 magnification. The Xfect Protein Transfection Reagent displayed a markedly higher signal for β-gal. (B) MDA-MB-231 cell viability was measured using the Cell Counting Kit-8 assay for four days. The viability of the anti-HURP Ab-transfected cells was lower than that of the parent cells. The cells transfected with 5μg anti-HURP Ab grew slower than the other transfected cells (*P<0.05 and **P<0.01).

Mentions: To investigate the transient effects of HURP and the possibility of McAb or polypeptide drug treatment, active anti-HURP Ab was transfected directly into the MDA-MB-231 cells. As the control transfection, the MDA-MB-231 cells were transfected with 2μg β-galactosidase (β-gal), which revealed a high efficiency and a high amount of β-gal protein per MDA-MB-231 cell (Fig. 3A). Cell proliferation analysis revealed that anti-HURP Ab-mediated disruption of HURP activity significantly inhibited cell growth. The higher the amount of anti-HURP Ab transfected into the MDA-MB-231 cells, the slower the cells grew (Fig. 3B).


Hepatoma upregulated protein expression is involved in the pathogenesis of human breast carcinogenesis.

Chen J, Liu QJ, Wang D, Zhou XY, Xiong D, Li HJ, Li CL - Oncol Lett (2014)

Interference in hepatoma upregulated protein (HURP) expression inhibits breast cancer cell proliferation in vitro. (A) MDA-MB-231 cells transfected with 2μg β-galactosidase (β-gal) using Xfect Protein Transfection Reagent. At 1 h post-transfection, the cells were assayed for β-gal activity using a β-Galactosidase Staining kit. The image was captured using an inverted microscope with ×100 magnification. The Xfect Protein Transfection Reagent displayed a markedly higher signal for β-gal. (B) MDA-MB-231 cell viability was measured using the Cell Counting Kit-8 assay for four days. The viability of the anti-HURP Ab-transfected cells was lower than that of the parent cells. The cells transfected with 5μg anti-HURP Ab grew slower than the other transfected cells (*P<0.05 and **P<0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214440&req=5

f3-ol-08-06-2543: Interference in hepatoma upregulated protein (HURP) expression inhibits breast cancer cell proliferation in vitro. (A) MDA-MB-231 cells transfected with 2μg β-galactosidase (β-gal) using Xfect Protein Transfection Reagent. At 1 h post-transfection, the cells were assayed for β-gal activity using a β-Galactosidase Staining kit. The image was captured using an inverted microscope with ×100 magnification. The Xfect Protein Transfection Reagent displayed a markedly higher signal for β-gal. (B) MDA-MB-231 cell viability was measured using the Cell Counting Kit-8 assay for four days. The viability of the anti-HURP Ab-transfected cells was lower than that of the parent cells. The cells transfected with 5μg anti-HURP Ab grew slower than the other transfected cells (*P<0.05 and **P<0.01).
Mentions: To investigate the transient effects of HURP and the possibility of McAb or polypeptide drug treatment, active anti-HURP Ab was transfected directly into the MDA-MB-231 cells. As the control transfection, the MDA-MB-231 cells were transfected with 2μg β-galactosidase (β-gal), which revealed a high efficiency and a high amount of β-gal protein per MDA-MB-231 cell (Fig. 3A). Cell proliferation analysis revealed that anti-HURP Ab-mediated disruption of HURP activity significantly inhibited cell growth. The higher the amount of anti-HURP Ab transfected into the MDA-MB-231 cells, the slower the cells grew (Fig. 3B).

Bottom Line: In the last decade, the overexpression of hepatoma upregulated protein (HURP) has been reported in hepatocellular carcinoma, adrenocortical tumors and urogenital carcinoma.The correlation between the HURP expression level and the clinicopathological characteristics was evaluated.The HURP expression level was higher in the tumors with advanced-grade metastasis and was strongly associated with tumor-node-metastasis staging (P=0.003).

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, Sichuan University, Chengdu, Sichuan 610072, P.R. China.

ABSTRACT
In the last decade, the overexpression of hepatoma upregulated protein (HURP) has been reported in hepatocellular carcinoma, adrenocortical tumors and urogenital carcinoma. However, the role of HURP in breast cancer remains unknown. In the present study, a comprehensive analysis was performed to examine the HURP expression level in 43 breast cancer tumor samples and paired adjacent normal tissues. The correlation between the HURP expression level and the clinicopathological characteristics was evaluated. The role of HURP in breast cancer was investigated by quantitative polymerase chain reaction, western blot analysis and cell proliferation assays. HURP expression was found to be significantly increased in the breast cancer samples. The HURP expression level was higher in the tumors with advanced-grade metastasis and was strongly associated with tumor-node-metastasis staging (P=0.003). Transfection and cell proliferation assays suggested that the suppression of HURP expression or the interference in HURP activity in the breast cancer cells inhibited cell proliferation significantly. These data suggest that HURP is associated with the degree of malignancy and the proliferation of breast cancer. HURP could be a tumor biomarker for prognosis and a potential therapeutic drug target for human breast cancer.

No MeSH data available.


Related in: MedlinePlus