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Expression levels of tumor necrosis factor-α and the corresponding receptors are correlated with trauma severity.

Liu C, Tang J - Oncol Lett (2014)

Bottom Line: This study investigated the plasma levels of tumor necrosis factor α (TNF-α) and the expression levels of TNF receptors (TNFRs) in patients with multiple trauma, together with the association between the levels of this cytokine and these cytokine receptors with the severity of traumatic injury.The expression levels of the cytokine and the corresponding receptors were correlated with the ISS.TNF-α and TNFR expression levels remained significantly elevated for up to the third to fifth day following the traumatic injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Surgery, Jinshan Hospital, Fudan University, Shanghai 200540, P.R. China.

ABSTRACT
This study investigated the plasma levels of tumor necrosis factor α (TNF-α) and the expression levels of TNF receptors (TNFRs) in patients with multiple trauma, together with the association between the levels of this cytokine and these cytokine receptors with the severity of traumatic injury. Blood samples were obtained from 60 multiple trauma patients at hospital admission (within 2 h of injury), and 6-8 h and 1-5 days after admission. The plasma levels of TNF-α and TNFR1/TNFR2 were detected using enzyme immunoassay. TNFR1 and TNFR2 expression levels on leukocytes, including neutrophils, lymphocytes and monocytes, were determined by flow cytometry. Clinical parameters were determined by injury severity score (ISS). At hospital admission, the plasma TNF-α and soluble TNFR levels in the trauma patients were elevated compared with those of healthy controls. Increased expression levels of TNFR1 and TNFR2 were also detected on leukocytes, particularly on lymphocytes and monocytes. The expression levels of the cytokine and the corresponding receptors were correlated with the ISS. TNF-α and TNFR expression levels remained significantly elevated for up to the third to fifth day following the traumatic injury. In the trauma patients, increased levels of TNF-α and TNFRs were correlated with the severity of traumatic injury in the early post-injury period, supporting the hypothesis that trauma-provoked organ dysfunction may be caused by an overwhelming auto-destructive inflammatory response.

No MeSH data available.


Related in: MedlinePlus

Plasma levels of sTNFR1 in patients with severe trauma. Traumatic injury upregulated the expression of the soluble receptor, with peak levels 1 day after trauma. Patients in the H-ISS group exhibited the highest sTNFR1 plasma levels at all five time points examined. Data are presented as the means ± SD, n=20 per group. *P<0.01, compared with the healthy controls. sTNFR 1, soluble tumor necrosis factor receptor 1; L/M/H-ISS, low/medium/high injury severity score.
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f2-ol-08-06-2747: Plasma levels of sTNFR1 in patients with severe trauma. Traumatic injury upregulated the expression of the soluble receptor, with peak levels 1 day after trauma. Patients in the H-ISS group exhibited the highest sTNFR1 plasma levels at all five time points examined. Data are presented as the means ± SD, n=20 per group. *P<0.01, compared with the healthy controls. sTNFR 1, soluble tumor necrosis factor receptor 1; L/M/H-ISS, low/medium/high injury severity score.

Mentions: Within 2 h of injury, the sTNFR1 and sTNFR2 plasma levels were significantly elevated in all trauma groups compared with the normal controls (1.83±0.23 and 1.46±0.42 pg/ml for sTNFR1 and sTNFR2 in the controls, respectively). The patient group with the highest severity score (H-ISS group) exhibited the highest sTNFR1 and sTNFR2 plasma levels in the early phase following trauma. Elevated expression levels of soluble receptors were also observed 8–10 h after injury and 1, 3 and 5 days after trauma. sTNFR1 expression reached peak levels one day after trauma, which was gradually reduced and returned to normal five days after trauma (Fig. 2). Although increased levels of sTNFR2 were also detected in trauma patients compared with the controls, sTNFR2 levels were elevated for variable periods of time and were dependent on the severity of injury (Fig. 3). The statistical analysis suggested a significant correlation between the plasma levels of the sTNFRs and the severity of traumatic injury upon hospital admission (sTNFR1: r=0.89, P<0.0001 and sTNFR2: r=0.92, P<0.0001) as well as at the other four time points (data not shown).


Expression levels of tumor necrosis factor-α and the corresponding receptors are correlated with trauma severity.

Liu C, Tang J - Oncol Lett (2014)

Plasma levels of sTNFR1 in patients with severe trauma. Traumatic injury upregulated the expression of the soluble receptor, with peak levels 1 day after trauma. Patients in the H-ISS group exhibited the highest sTNFR1 plasma levels at all five time points examined. Data are presented as the means ± SD, n=20 per group. *P<0.01, compared with the healthy controls. sTNFR 1, soluble tumor necrosis factor receptor 1; L/M/H-ISS, low/medium/high injury severity score.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214438&req=5

f2-ol-08-06-2747: Plasma levels of sTNFR1 in patients with severe trauma. Traumatic injury upregulated the expression of the soluble receptor, with peak levels 1 day after trauma. Patients in the H-ISS group exhibited the highest sTNFR1 plasma levels at all five time points examined. Data are presented as the means ± SD, n=20 per group. *P<0.01, compared with the healthy controls. sTNFR 1, soluble tumor necrosis factor receptor 1; L/M/H-ISS, low/medium/high injury severity score.
Mentions: Within 2 h of injury, the sTNFR1 and sTNFR2 plasma levels were significantly elevated in all trauma groups compared with the normal controls (1.83±0.23 and 1.46±0.42 pg/ml for sTNFR1 and sTNFR2 in the controls, respectively). The patient group with the highest severity score (H-ISS group) exhibited the highest sTNFR1 and sTNFR2 plasma levels in the early phase following trauma. Elevated expression levels of soluble receptors were also observed 8–10 h after injury and 1, 3 and 5 days after trauma. sTNFR1 expression reached peak levels one day after trauma, which was gradually reduced and returned to normal five days after trauma (Fig. 2). Although increased levels of sTNFR2 were also detected in trauma patients compared with the controls, sTNFR2 levels were elevated for variable periods of time and were dependent on the severity of injury (Fig. 3). The statistical analysis suggested a significant correlation between the plasma levels of the sTNFRs and the severity of traumatic injury upon hospital admission (sTNFR1: r=0.89, P<0.0001 and sTNFR2: r=0.92, P<0.0001) as well as at the other four time points (data not shown).

Bottom Line: This study investigated the plasma levels of tumor necrosis factor α (TNF-α) and the expression levels of TNF receptors (TNFRs) in patients with multiple trauma, together with the association between the levels of this cytokine and these cytokine receptors with the severity of traumatic injury.The expression levels of the cytokine and the corresponding receptors were correlated with the ISS.TNF-α and TNFR expression levels remained significantly elevated for up to the third to fifth day following the traumatic injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Surgery, Jinshan Hospital, Fudan University, Shanghai 200540, P.R. China.

ABSTRACT
This study investigated the plasma levels of tumor necrosis factor α (TNF-α) and the expression levels of TNF receptors (TNFRs) in patients with multiple trauma, together with the association between the levels of this cytokine and these cytokine receptors with the severity of traumatic injury. Blood samples were obtained from 60 multiple trauma patients at hospital admission (within 2 h of injury), and 6-8 h and 1-5 days after admission. The plasma levels of TNF-α and TNFR1/TNFR2 were detected using enzyme immunoassay. TNFR1 and TNFR2 expression levels on leukocytes, including neutrophils, lymphocytes and monocytes, were determined by flow cytometry. Clinical parameters were determined by injury severity score (ISS). At hospital admission, the plasma TNF-α and soluble TNFR levels in the trauma patients were elevated compared with those of healthy controls. Increased expression levels of TNFR1 and TNFR2 were also detected on leukocytes, particularly on lymphocytes and monocytes. The expression levels of the cytokine and the corresponding receptors were correlated with the ISS. TNF-α and TNFR expression levels remained significantly elevated for up to the third to fifth day following the traumatic injury. In the trauma patients, increased levels of TNF-α and TNFRs were correlated with the severity of traumatic injury in the early post-injury period, supporting the hypothesis that trauma-provoked organ dysfunction may be caused by an overwhelming auto-destructive inflammatory response.

No MeSH data available.


Related in: MedlinePlus