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Survivin and vascular endothelial growth factor are associated with spontaneous pulmonary metastasis of osteosarcoma: Development of an orthotopic mouse model.

Zhang L, Ye Y, Yang D, Lin J - Oncol Lett (2014)

Bottom Line: The high rate of pulmonary metastases of osteosarcoma (OS) presents a therapeutic challenge in the field of orthopedics.At three weeks, multiple nodules were observed in the lungs.The expression of survivin and vascular endothelial growth factor (VEGF) was analyzed in the tumors and lungs via immunohistochemistry.

View Article: PubMed Central - PubMed

Affiliation: College of Orthopedics and Traumatology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

ABSTRACT
The high rate of pulmonary metastases of osteosarcoma (OS) presents a therapeutic challenge in the field of orthopedics. Therefore, there is a marked requirement to establish a spontaneous pulmonary metastasis animal model of OS, within which potential antitumor agents may be evaluated for their ability to inhibit the growth and pulmonary metastasis of OS, as well as to identify potentially associated biomarkers of OS metastasis. In the present study, rodent OS cells (UMR106-01) were injected into the right tibia of athymic nude mice. The mice were sacrificed weekly by cervical dislocation at one to five weeks following inoculation. The orthotopic mice developed tumor masses in the right tibia one week following inoculation. At three weeks, multiple nodules were observed in the lungs. The expression of survivin and vascular endothelial growth factor (VEGF) was analyzed in the tumors and lungs via immunohistochemistry. The positive expression of survivin and VEGF was identified in the local tumor and lung tissue of the orthotopic mice, however was not observed in the tissues of the healthy control mice. The orthotopic model established in the current study presents a valuable tool for the investigation of factors that promote or inhibit OS growth and/or metastasis. In addition, it was identified that survivin and VEGF may be significant in the lung metastasis of OS.

No MeSH data available.


Related in: MedlinePlus

The macroscopic appearance of the orthotopic primary tumor from mice (A) one and (B) two weeks following inoculation. (C) A tumor growth curve, which presents the mean volume of the orthotopic primary tumors at various time points. (D) Macroscopic appearance of the orthotopic primary tumor five weeks following inoculation. (E) Histological characterization of the orthotopic primary tumor (stain, hematoxylin and eosin; magnification, ×200). (F) Multiple nodules were observed in the lungs at five weeks. (G) Histological characterization of one of the nodules visible in the lungs at five weeks (stain, hematoxylin and eosin; magnification, ×200).
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f1-ol-08-06-2577: The macroscopic appearance of the orthotopic primary tumor from mice (A) one and (B) two weeks following inoculation. (C) A tumor growth curve, which presents the mean volume of the orthotopic primary tumors at various time points. (D) Macroscopic appearance of the orthotopic primary tumor five weeks following inoculation. (E) Histological characterization of the orthotopic primary tumor (stain, hematoxylin and eosin; magnification, ×200). (F) Multiple nodules were observed in the lungs at five weeks. (G) Histological characterization of one of the nodules visible in the lungs at five weeks (stain, hematoxylin and eosin; magnification, ×200).

Mentions: Tibial tumors were observed macroscopically at the first week following inoculation (Fig. 1A; Table I). The tumor size had increased rapidly two weeks following inoculation (mean tumor volume, 0.76 cm3) and the skin overlying the tumor was red (Fig. 1B and C). After five weeks, a large, local tumor mass (mean tumor volume, 6.3 cm3) was observed (Fig. 1D). Microscopically, the tumor exhibited numerous pleomorphic matrix components consisting of osteoblasts and pleomorphic tumor cells (Fig. 1E). No lung metastases were identified macroscopically after one or two weeks. However, macroscopic nodules were visible in the lungs at weeks three (4/5 mice), four (5/5 mice) and five (5/5 mice) (Fig. 1F and G; Table I).


Survivin and vascular endothelial growth factor are associated with spontaneous pulmonary metastasis of osteosarcoma: Development of an orthotopic mouse model.

Zhang L, Ye Y, Yang D, Lin J - Oncol Lett (2014)

The macroscopic appearance of the orthotopic primary tumor from mice (A) one and (B) two weeks following inoculation. (C) A tumor growth curve, which presents the mean volume of the orthotopic primary tumors at various time points. (D) Macroscopic appearance of the orthotopic primary tumor five weeks following inoculation. (E) Histological characterization of the orthotopic primary tumor (stain, hematoxylin and eosin; magnification, ×200). (F) Multiple nodules were observed in the lungs at five weeks. (G) Histological characterization of one of the nodules visible in the lungs at five weeks (stain, hematoxylin and eosin; magnification, ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214415&req=5

f1-ol-08-06-2577: The macroscopic appearance of the orthotopic primary tumor from mice (A) one and (B) two weeks following inoculation. (C) A tumor growth curve, which presents the mean volume of the orthotopic primary tumors at various time points. (D) Macroscopic appearance of the orthotopic primary tumor five weeks following inoculation. (E) Histological characterization of the orthotopic primary tumor (stain, hematoxylin and eosin; magnification, ×200). (F) Multiple nodules were observed in the lungs at five weeks. (G) Histological characterization of one of the nodules visible in the lungs at five weeks (stain, hematoxylin and eosin; magnification, ×200).
Mentions: Tibial tumors were observed macroscopically at the first week following inoculation (Fig. 1A; Table I). The tumor size had increased rapidly two weeks following inoculation (mean tumor volume, 0.76 cm3) and the skin overlying the tumor was red (Fig. 1B and C). After five weeks, a large, local tumor mass (mean tumor volume, 6.3 cm3) was observed (Fig. 1D). Microscopically, the tumor exhibited numerous pleomorphic matrix components consisting of osteoblasts and pleomorphic tumor cells (Fig. 1E). No lung metastases were identified macroscopically after one or two weeks. However, macroscopic nodules were visible in the lungs at weeks three (4/5 mice), four (5/5 mice) and five (5/5 mice) (Fig. 1F and G; Table I).

Bottom Line: The high rate of pulmonary metastases of osteosarcoma (OS) presents a therapeutic challenge in the field of orthopedics.At three weeks, multiple nodules were observed in the lungs.The expression of survivin and vascular endothelial growth factor (VEGF) was analyzed in the tumors and lungs via immunohistochemistry.

View Article: PubMed Central - PubMed

Affiliation: College of Orthopedics and Traumatology, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.

ABSTRACT
The high rate of pulmonary metastases of osteosarcoma (OS) presents a therapeutic challenge in the field of orthopedics. Therefore, there is a marked requirement to establish a spontaneous pulmonary metastasis animal model of OS, within which potential antitumor agents may be evaluated for their ability to inhibit the growth and pulmonary metastasis of OS, as well as to identify potentially associated biomarkers of OS metastasis. In the present study, rodent OS cells (UMR106-01) were injected into the right tibia of athymic nude mice. The mice were sacrificed weekly by cervical dislocation at one to five weeks following inoculation. The orthotopic mice developed tumor masses in the right tibia one week following inoculation. At three weeks, multiple nodules were observed in the lungs. The expression of survivin and vascular endothelial growth factor (VEGF) was analyzed in the tumors and lungs via immunohistochemistry. The positive expression of survivin and VEGF was identified in the local tumor and lung tissue of the orthotopic mice, however was not observed in the tissues of the healthy control mice. The orthotopic model established in the current study presents a valuable tool for the investigation of factors that promote or inhibit OS growth and/or metastasis. In addition, it was identified that survivin and VEGF may be significant in the lung metastasis of OS.

No MeSH data available.


Related in: MedlinePlus