Limits...
Clinical significance of migration and invasion inhibitor protein expression in non-small-cell lung cancer.

Wang X, Liu H, Wang X, An Y - Oncol Lett (2014)

Bottom Line: The results revealed that MIIP mRNA and protein expression were downregulated in cancer tissues, as compared with the matched normal tissues.MIIP expression levels were significantly associated with pathology and tumor stage, with reduced MIIP mRNA expression levels detected in advanced tumor stage samples.Furthermore, patients with MIIP-positive protein expression had an improved prognosis as compared with those patients with MIIP-negative protein expression, with five-year survival rates of 41.7 and 22.4%, respectively (Kaplan-Meier, log-rank, P=0.028).

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.

ABSTRACT
Migration and invasion inhibitor protein (MIIP) was initially identified in a yeast two-hybrid screen. Recently, MIIP has emerged as a key protein in regulating cell migration and invasion. However, the MIIP expression profile in non-small-cell lung cancer (NSCLC) has not been analyzed. In the present study, MIIP mRNA expression levels were evaluated using the SYBR Green quantitative real-time polymerase chain reaction method in 37 NSCLC specimens and matched normal tissue samples. MIIP protein expression in a further 94 NSCLC specimens was examined with immunohistochemistry. Patient survival data were collected retrospectively, and the association between MIIP protein expression and the five-year overall survival rate was evaluated. The results revealed that MIIP mRNA and protein expression were downregulated in cancer tissues, as compared with the matched normal tissues. MIIP expression levels were significantly associated with pathology and tumor stage, with reduced MIIP mRNA expression levels detected in advanced tumor stage samples. Furthermore, patients with MIIP-positive protein expression had an improved prognosis as compared with those patients with MIIP-negative protein expression, with five-year survival rates of 41.7 and 22.4%, respectively (Kaplan-Meier, log-rank, P=0.028). A significant association between MIIP protein expression and improved prognosis was also demonstrated using univariate and multivariate analyses (P=0.033 and P=0.040, respectively). These results suggest that MIIP may have a potential role in the pathogenesis of NSCLC and also confirm that MIIP is a putative tumor-suppressor gene. Therefore, MIIP may be identified as a functional genetic marker of NSCLC development and prognosis, and may be an attractive therapeutic target for the treatment of lung cancer.

No MeSH data available.


Related in: MedlinePlus

Migration and invasion inhibitor protein (MIIP) expression within tumor stage groups. (A) The relative MIIP mRNA expression levels in normal tissues and tumor tissues at different stages; (B) the percentage of positive MIIP protein expression in all patients and in patients with tumors at different stages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4214401&req=5

f2-ol-08-06-2417: Migration and invasion inhibitor protein (MIIP) expression within tumor stage groups. (A) The relative MIIP mRNA expression levels in normal tissues and tumor tissues at different stages; (B) the percentage of positive MIIP protein expression in all patients and in patients with tumors at different stages.

Mentions: The association between MIIP mRNA expression levels in the cancer tissues and various clinicopathological parameters was further analyzed. The results revealed that the MIIP mRNA expression levels in adenocarcinoma were significantly higher than those in squamous cell carcinoma (P=0.002). A statistically significant correlation was also observed between MIIP mRNA expression and tumor stage (P=0.014), with reduced MIIP mRNA expression levels in advanced tumor stage samples, as compared with specimens from tumors at the lower stages (Fig. 2A). However, no significant correlations were observed between MIIP expression levels and gender, age or differentiation status (all P>0.05), as shown in Table II.


Clinical significance of migration and invasion inhibitor protein expression in non-small-cell lung cancer.

Wang X, Liu H, Wang X, An Y - Oncol Lett (2014)

Migration and invasion inhibitor protein (MIIP) expression within tumor stage groups. (A) The relative MIIP mRNA expression levels in normal tissues and tumor tissues at different stages; (B) the percentage of positive MIIP protein expression in all patients and in patients with tumors at different stages.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214401&req=5

f2-ol-08-06-2417: Migration and invasion inhibitor protein (MIIP) expression within tumor stage groups. (A) The relative MIIP mRNA expression levels in normal tissues and tumor tissues at different stages; (B) the percentage of positive MIIP protein expression in all patients and in patients with tumors at different stages.
Mentions: The association between MIIP mRNA expression levels in the cancer tissues and various clinicopathological parameters was further analyzed. The results revealed that the MIIP mRNA expression levels in adenocarcinoma were significantly higher than those in squamous cell carcinoma (P=0.002). A statistically significant correlation was also observed between MIIP mRNA expression and tumor stage (P=0.014), with reduced MIIP mRNA expression levels in advanced tumor stage samples, as compared with specimens from tumors at the lower stages (Fig. 2A). However, no significant correlations were observed between MIIP expression levels and gender, age or differentiation status (all P>0.05), as shown in Table II.

Bottom Line: The results revealed that MIIP mRNA and protein expression were downregulated in cancer tissues, as compared with the matched normal tissues.MIIP expression levels were significantly associated with pathology and tumor stage, with reduced MIIP mRNA expression levels detected in advanced tumor stage samples.Furthermore, patients with MIIP-positive protein expression had an improved prognosis as compared with those patients with MIIP-negative protein expression, with five-year survival rates of 41.7 and 22.4%, respectively (Kaplan-Meier, log-rank, P=0.028).

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121000, P.R. China.

ABSTRACT
Migration and invasion inhibitor protein (MIIP) was initially identified in a yeast two-hybrid screen. Recently, MIIP has emerged as a key protein in regulating cell migration and invasion. However, the MIIP expression profile in non-small-cell lung cancer (NSCLC) has not been analyzed. In the present study, MIIP mRNA expression levels were evaluated using the SYBR Green quantitative real-time polymerase chain reaction method in 37 NSCLC specimens and matched normal tissue samples. MIIP protein expression in a further 94 NSCLC specimens was examined with immunohistochemistry. Patient survival data were collected retrospectively, and the association between MIIP protein expression and the five-year overall survival rate was evaluated. The results revealed that MIIP mRNA and protein expression were downregulated in cancer tissues, as compared with the matched normal tissues. MIIP expression levels were significantly associated with pathology and tumor stage, with reduced MIIP mRNA expression levels detected in advanced tumor stage samples. Furthermore, patients with MIIP-positive protein expression had an improved prognosis as compared with those patients with MIIP-negative protein expression, with five-year survival rates of 41.7 and 22.4%, respectively (Kaplan-Meier, log-rank, P=0.028). A significant association between MIIP protein expression and improved prognosis was also demonstrated using univariate and multivariate analyses (P=0.033 and P=0.040, respectively). These results suggest that MIIP may have a potential role in the pathogenesis of NSCLC and also confirm that MIIP is a putative tumor-suppressor gene. Therefore, MIIP may be identified as a functional genetic marker of NSCLC development and prognosis, and may be an attractive therapeutic target for the treatment of lung cancer.

No MeSH data available.


Related in: MedlinePlus