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Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

Liu MW, Wang YH, Qian CY, Li H - Int. J. Mol. Med. (2014)

Bottom Line: Additionally, the number of neutrophils and total cells were significantly decreased in the XBJ group compared to that in the control group.The histological results also demonstrated the attenuation effect of XBJ on CLP-induced lung inflammation.The results of the present study indicated that XBJ has a significantly reduced CLP-induced lung permeability by upregulating Tollip expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, The First Hospital Affiliated To Kunming Medical University, Kunming, Yunnan 650000, P.R. China.

ABSTRACT
Xuebijing (XBJ) is a type of traditional Tibetan medicine, and previous pharmacological studies have shown that the ethanol extract is derived from Chuanxiong, Chishao, Danshen and Honghua. Chuanxiong, Chishao, Danshen and Honghua possesses potent anti-inflammatory activity, and has been used in the treatment of inflammatory infectious diseases. In the present study, we investigated the effects of XBJ on pulmonary permeability and lung injury in cecal ligation and puncture (CLP)-induced sepsis in rats. A CLP sepsis model was established for the control and treatment groups, respectively. Approximately 2 h prior to surgery, an amount of 100 mg/kg XBJ injection was administered to the treatment group. Reverse transcription polymerase chain reaction (PT-PCR) and western blot analysis were used to examine the expression of Toll-interacting protein (Tollip), interleukin-1 receptor-associated kinase 1 (IRAK1), Toll-like receptor 4 (TLR4), nuclear factor-κB65 (NF-κB65) and TNF receptor-associated factor 6 (TRAF6) in lung tissue. ELISA was applied to detect changes of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 (IL-1), interleukin-4 (IL-4) and interleukin-10 (IL-10) levels in bronchoalveolar lavage (BAL) fluid, and intercellular adhesion molecule 1 (ICAM-1) and von wille-brand factor (vWF) in serum. The number of neutrophils, albumin and total cells in the BAL fluid were measured. For histological analysis, hematoxylin and eosin (H&E) stains were evaluated. Lung permeability, the wet/dry weight ratio (W/D) and the lung pathology score were determined following the induction of ALI by CLP for 24 h. The results demonstrated that XBJ upregulated Tollip expression and blocked the activity of IRAK1, TLR4, NF-κβ65 and TRAF6. Additionally, the number of neutrophils and total cells were significantly decreased in the XBJ group compared to that in the control group. Lung permeability, the wet/dry weight ratio (W/D) and the lung pathology score were significantly decreased in the XBJ group. The histological results also demonstrated the attenuation effect of XBJ on CLP-induced lung inflammation. The results of the present study indicated that XBJ has a significantly reduced CLP-induced lung permeability by upregulating Tollip expression. The protective effects of XBJ suggest its therapeutic potential in CLP-induced acute lung injury treatment.

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XBJ reduced the CLP-induced lung permeability. Groups of rats were treated as described in Materials and methods. FITC-labeled albumin in the BAL fluid, wet/dry lung weight ratio and lung water content in lung tissue were determined at 24 h after the CLP challenge. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. *P<0.05, **P<0.01 vs. normal control and sham operation groups; #P<0.05, ##P<0.01 vs. control group.
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f11-ijmm-34-06-1492: XBJ reduced the CLP-induced lung permeability. Groups of rats were treated as described in Materials and methods. FITC-labeled albumin in the BAL fluid, wet/dry lung weight ratio and lung water content in lung tissue were determined at 24 h after the CLP challenge. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. *P<0.05, **P<0.01 vs. normal control and sham operation groups; #P<0.05, ##P<0.01 vs. control group.

Mentions: FITC-labeled albumin in BAL, wet/dry lung weight ratio and the water content in lung tissue were reliable parameters of pulmonary permeability. To identify the effect of XBJ administration on pulmonary permeability in septic rats, FITC-labeled albumin in BAL, wet/dry lung weight ratio and the water conten in lung tissue were assayed. As shown in Fig. 11, FITC-labeled albumin in BAL, wet/dry lung weight ratio and the water conteny in lung tissue were increased significantly in CLP-induced rats. However, the increase of FITC-labeled albumin in BAL, wet/dry lung weight ratio and the water content in lung tissue were decreased by XBJ. Therefore, the effects of pulmonary permeability were significantly blocked by XBJ (P<0.05; Fig. 11).


Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis.

Liu MW, Wang YH, Qian CY, Li H - Int. J. Mol. Med. (2014)

XBJ reduced the CLP-induced lung permeability. Groups of rats were treated as described in Materials and methods. FITC-labeled albumin in the BAL fluid, wet/dry lung weight ratio and lung water content in lung tissue were determined at 24 h after the CLP challenge. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. *P<0.05, **P<0.01 vs. normal control and sham operation groups; #P<0.05, ##P<0.01 vs. control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214342&req=5

f11-ijmm-34-06-1492: XBJ reduced the CLP-induced lung permeability. Groups of rats were treated as described in Materials and methods. FITC-labeled albumin in the BAL fluid, wet/dry lung weight ratio and lung water content in lung tissue were determined at 24 h after the CLP challenge. Data are presented as mean ± standard deviation of one experiment consisting of three replicates. *P<0.05, **P<0.01 vs. normal control and sham operation groups; #P<0.05, ##P<0.01 vs. control group.
Mentions: FITC-labeled albumin in BAL, wet/dry lung weight ratio and the water content in lung tissue were reliable parameters of pulmonary permeability. To identify the effect of XBJ administration on pulmonary permeability in septic rats, FITC-labeled albumin in BAL, wet/dry lung weight ratio and the water conten in lung tissue were assayed. As shown in Fig. 11, FITC-labeled albumin in BAL, wet/dry lung weight ratio and the water conteny in lung tissue were increased significantly in CLP-induced rats. However, the increase of FITC-labeled albumin in BAL, wet/dry lung weight ratio and the water content in lung tissue were decreased by XBJ. Therefore, the effects of pulmonary permeability were significantly blocked by XBJ (P<0.05; Fig. 11).

Bottom Line: Additionally, the number of neutrophils and total cells were significantly decreased in the XBJ group compared to that in the control group.The histological results also demonstrated the attenuation effect of XBJ on CLP-induced lung inflammation.The results of the present study indicated that XBJ has a significantly reduced CLP-induced lung permeability by upregulating Tollip expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency, The First Hospital Affiliated To Kunming Medical University, Kunming, Yunnan 650000, P.R. China.

ABSTRACT
Xuebijing (XBJ) is a type of traditional Tibetan medicine, and previous pharmacological studies have shown that the ethanol extract is derived from Chuanxiong, Chishao, Danshen and Honghua. Chuanxiong, Chishao, Danshen and Honghua possesses potent anti-inflammatory activity, and has been used in the treatment of inflammatory infectious diseases. In the present study, we investigated the effects of XBJ on pulmonary permeability and lung injury in cecal ligation and puncture (CLP)-induced sepsis in rats. A CLP sepsis model was established for the control and treatment groups, respectively. Approximately 2 h prior to surgery, an amount of 100 mg/kg XBJ injection was administered to the treatment group. Reverse transcription polymerase chain reaction (PT-PCR) and western blot analysis were used to examine the expression of Toll-interacting protein (Tollip), interleukin-1 receptor-associated kinase 1 (IRAK1), Toll-like receptor 4 (TLR4), nuclear factor-κB65 (NF-κB65) and TNF receptor-associated factor 6 (TRAF6) in lung tissue. ELISA was applied to detect changes of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 (IL-1), interleukin-4 (IL-4) and interleukin-10 (IL-10) levels in bronchoalveolar lavage (BAL) fluid, and intercellular adhesion molecule 1 (ICAM-1) and von wille-brand factor (vWF) in serum. The number of neutrophils, albumin and total cells in the BAL fluid were measured. For histological analysis, hematoxylin and eosin (H&E) stains were evaluated. Lung permeability, the wet/dry weight ratio (W/D) and the lung pathology score were determined following the induction of ALI by CLP for 24 h. The results demonstrated that XBJ upregulated Tollip expression and blocked the activity of IRAK1, TLR4, NF-κβ65 and TRAF6. Additionally, the number of neutrophils and total cells were significantly decreased in the XBJ group compared to that in the control group. Lung permeability, the wet/dry weight ratio (W/D) and the lung pathology score were significantly decreased in the XBJ group. The histological results also demonstrated the attenuation effect of XBJ on CLP-induced lung inflammation. The results of the present study indicated that XBJ has a significantly reduced CLP-induced lung permeability by upregulating Tollip expression. The protective effects of XBJ suggest its therapeutic potential in CLP-induced acute lung injury treatment.

Show MeSH
Related in: MedlinePlus