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Increased gastric chromogranin A cell density following changes to diets of patients with irritable bowel syndrome.

Mazzawi T, Gundersen D, Hausken T, El-Salhy M - Mol Med Rep (2014)

Bottom Line: The present study was undertaken to establish whether the positive outcome of dietary guidance observed in patients suffering from IBS is associated with a change in gastric endocrine cells.The patients with IBS presented a low density of CgA compared with the controls.The current study also suggests that the positive effects of dietary guidance may be attributed to changes in gut endocrine cell density.

View Article: PubMed Central - PubMed

Affiliation: Section for Gastroenterology, Department of Medicine, Stord Helse‑Fonna Hospital, Stord, Norway.

ABSTRACT
The gut endocrine cells control and regulate several functions of the gastrointestinal tract. They have been reported to be abnormal in irritable bowel syndrome (IBS), with alterations occurring in several functions regulated by these cells. Furthermore, it has been established that gut endocrine cells interact with the gut lumen contents, particularly the nutrients. The present study was undertaken to establish whether the positive outcome of dietary guidance observed in patients suffering from IBS is associated with a change in gastric endocrine cells. A total of 46 patients with IBS participated in the present study, of which 14 completed all aspects. These patients included nine females and five males with a mean age of 34 years (range, 20‑45 years). In the healthy control group, nine females and five males, with a mean age of 54 years (range 26‑70 years) were selected. The patients and controls underwent gastroscopy with biopsy samples taken from the corpus and antrum of the stomach. Each patient attended three sessions that lasted ~45 min each, and received individual guidance on their dietary management. The patients followed the diet prescribed for a minimum of three months, then further samples were taken using a method similar to that used for the initial biopsies. The biopsy samples were immunostained using the avidin‑biotin complex method for chromogranin A (CgA) and quantified by computerized image analysis. The patients with IBS presented a low density of CgA compared with the controls. The density of CgA increased in these patients following dietary guidance and changes in food intake. The present observations emphasized the interaction between food intake and gut endocrine cells. The current study also suggests that the positive effects of dietary guidance may be attributed to changes in gut endocrine cell density.

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CgA immunoreactive cells in the corpus of (A) a control subject and an IBS patient (B) prior to and (C) following guidance on dietary management. The density of CgA cells prior to diet management is lower in IBS patients compared with controls, whereas the density in patients with IBS is almost the same as in controls following diet management. CgA, chromogranin A; IBS, irritable bowel syndrome.
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f2-mmr-10-05-2322: CgA immunoreactive cells in the corpus of (A) a control subject and an IBS patient (B) prior to and (C) following guidance on dietary management. The density of CgA cells prior to diet management is lower in IBS patients compared with controls, whereas the density in patients with IBS is almost the same as in controls following diet management. CgA, chromogranin A; IBS, irritable bowel syndrome.

Mentions: The mean density of CgA-secreting cells in controls was 147.9 cells/mm2 (95% CI: 113.8–182). The densities of the CgA-secreting cells in patients with IBS prior to and following dietary guidance were 62.6±9.3 and 102.3±14.3 cells/mm2, respectively (Figs. 1 and 2). The paired t-test indicated a significant increase in the densities of CgA-secreting cells in IBS patients following dietary guidance (P=0.0064).


Increased gastric chromogranin A cell density following changes to diets of patients with irritable bowel syndrome.

Mazzawi T, Gundersen D, Hausken T, El-Salhy M - Mol Med Rep (2014)

CgA immunoreactive cells in the corpus of (A) a control subject and an IBS patient (B) prior to and (C) following guidance on dietary management. The density of CgA cells prior to diet management is lower in IBS patients compared with controls, whereas the density in patients with IBS is almost the same as in controls following diet management. CgA, chromogranin A; IBS, irritable bowel syndrome.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214334&req=5

f2-mmr-10-05-2322: CgA immunoreactive cells in the corpus of (A) a control subject and an IBS patient (B) prior to and (C) following guidance on dietary management. The density of CgA cells prior to diet management is lower in IBS patients compared with controls, whereas the density in patients with IBS is almost the same as in controls following diet management. CgA, chromogranin A; IBS, irritable bowel syndrome.
Mentions: The mean density of CgA-secreting cells in controls was 147.9 cells/mm2 (95% CI: 113.8–182). The densities of the CgA-secreting cells in patients with IBS prior to and following dietary guidance were 62.6±9.3 and 102.3±14.3 cells/mm2, respectively (Figs. 1 and 2). The paired t-test indicated a significant increase in the densities of CgA-secreting cells in IBS patients following dietary guidance (P=0.0064).

Bottom Line: The present study was undertaken to establish whether the positive outcome of dietary guidance observed in patients suffering from IBS is associated with a change in gastric endocrine cells.The patients with IBS presented a low density of CgA compared with the controls.The current study also suggests that the positive effects of dietary guidance may be attributed to changes in gut endocrine cell density.

View Article: PubMed Central - PubMed

Affiliation: Section for Gastroenterology, Department of Medicine, Stord Helse‑Fonna Hospital, Stord, Norway.

ABSTRACT
The gut endocrine cells control and regulate several functions of the gastrointestinal tract. They have been reported to be abnormal in irritable bowel syndrome (IBS), with alterations occurring in several functions regulated by these cells. Furthermore, it has been established that gut endocrine cells interact with the gut lumen contents, particularly the nutrients. The present study was undertaken to establish whether the positive outcome of dietary guidance observed in patients suffering from IBS is associated with a change in gastric endocrine cells. A total of 46 patients with IBS participated in the present study, of which 14 completed all aspects. These patients included nine females and five males with a mean age of 34 years (range, 20‑45 years). In the healthy control group, nine females and five males, with a mean age of 54 years (range 26‑70 years) were selected. The patients and controls underwent gastroscopy with biopsy samples taken from the corpus and antrum of the stomach. Each patient attended three sessions that lasted ~45 min each, and received individual guidance on their dietary management. The patients followed the diet prescribed for a minimum of three months, then further samples were taken using a method similar to that used for the initial biopsies. The biopsy samples were immunostained using the avidin‑biotin complex method for chromogranin A (CgA) and quantified by computerized image analysis. The patients with IBS presented a low density of CgA compared with the controls. The density of CgA increased in these patients following dietary guidance and changes in food intake. The present observations emphasized the interaction between food intake and gut endocrine cells. The current study also suggests that the positive effects of dietary guidance may be attributed to changes in gut endocrine cell density.

Show MeSH
Related in: MedlinePlus