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Drug resistance in Salmonella enterica ser. Typhimurium bloodstream infection, Malawi.

Feasey NA, Cain AK, Msefula CL, Pickard D, Alaerts M, Aslett M, Everett DB, Allain TJ, Dougan G, Gordon MA, Heyderman RS, Kingsley RA - Emerging Infect. Dis. (2014)

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Salmonella enterica serotype Typhimurium is one of the most common causes of bloodstream infection in sub-Saharan Africa... Epidemics of iNTS disease in sub-Saharan Africa have been associated with a novel genotype of S. enterica ser... Typhimurium of multilocus sequence type (ST) 313 that is rarely seen outside the region and is associated with multidrug resistance (MDR) to chloramphenicol, cotrimoxazole, and ampicillin... Typhimurium bloodstream infection with additional resistance to ceftriaxone and ciprofloxacin... In the absence of a locally available effective antimicrobial drug, she was treated with ceftriaxone, gentamicin, and high-dose ciprofloxacin but died shortly thereafter... Typhimurium strains A54285 (initial presentation) and A54560 (recurrence); both isolates were resistant to ampicillin, chloramphenicol, and cotrimoxazole, but A54560 exhibited additional resistance to ceftriaxone, ciprofloxacin, and tetracycline... Plasmid extraction and gel electrophoresis of genomic DNA identified a plasmid migrating in the gel to a position approximately equivalent to 120 kb, the size of ST313 virulence plasmid pSLT-BT in both strains, but no 300-kb plasmid was visualized in the ceftriaxone- and ciprofloxacin-resistant strain (A54560, data not shown), possibly because of the difficulty large plasmids have entering standard 1% agarose gels... We identified 331 predicted coding sequences, including 109 genes required for replication and transfer and 61 genes predicted to be associated with metabolism, membranes, virulence, antimicrobial resistance, and a toxin/antitoxin addiction system... We found an additional 160 predicted, hypothetical genes... The spread of mobile genetic elements that confer antimicrobial resistance among gram-negative organisms is of considerable concern... Wide dissemination of this strain or the IncHI2 (pSTm-BTCR) plasmid among other salmonellae in sub-Saharan Africa would rapidly render iNTS effectively untreatable with currently available antibacterial drugs... Detailed methods and plasmid map of pSTm-BTCR.

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Midpoint-rooted phylogenetic tree of published whole-genome sequence data from D23580-like Salmonella enterica serotype Typhimurium sequence type 313s from Malawi based on 204 informative single-nucleotide polymorphisms. A54285 and A54560, highlighted in yellow on a red branch, are indistinguishable. Scale bar indicates nucleotide substitutions per site.
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Figure 1: Midpoint-rooted phylogenetic tree of published whole-genome sequence data from D23580-like Salmonella enterica serotype Typhimurium sequence type 313s from Malawi based on 204 informative single-nucleotide polymorphisms. A54285 and A54560, highlighted in yellow on a red branch, are indistinguishable. Scale bar indicates nucleotide substitutions per site.

Mentions: Paired-end sequencing of isolates A54285 (European Nucleotide Archive [ENA] accession number ERS035867) and A54560 (ENA accession no. ERS035866) that were cultured 1 month apart showed no differences between the conserved regions of these genomes (Figure). The similarity of these S.enterica ser. Typhimurium genomes strongly suggests that this recrudescence occurred after incomplete clearance of the first infection; although re-infection from the same source is unlikely, it cannot be excluded. Comparison of the accessory genomes, however, showed an additional 300 kb DNA in A54560.


Drug resistance in Salmonella enterica ser. Typhimurium bloodstream infection, Malawi.

Feasey NA, Cain AK, Msefula CL, Pickard D, Alaerts M, Aslett M, Everett DB, Allain TJ, Dougan G, Gordon MA, Heyderman RS, Kingsley RA - Emerging Infect. Dis. (2014)

Midpoint-rooted phylogenetic tree of published whole-genome sequence data from D23580-like Salmonella enterica serotype Typhimurium sequence type 313s from Malawi based on 204 informative single-nucleotide polymorphisms. A54285 and A54560, highlighted in yellow on a red branch, are indistinguishable. Scale bar indicates nucleotide substitutions per site.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4214322&req=5

Figure 1: Midpoint-rooted phylogenetic tree of published whole-genome sequence data from D23580-like Salmonella enterica serotype Typhimurium sequence type 313s from Malawi based on 204 informative single-nucleotide polymorphisms. A54285 and A54560, highlighted in yellow on a red branch, are indistinguishable. Scale bar indicates nucleotide substitutions per site.
Mentions: Paired-end sequencing of isolates A54285 (European Nucleotide Archive [ENA] accession number ERS035867) and A54560 (ENA accession no. ERS035866) that were cultured 1 month apart showed no differences between the conserved regions of these genomes (Figure). The similarity of these S.enterica ser. Typhimurium genomes strongly suggests that this recrudescence occurred after incomplete clearance of the first infection; although re-infection from the same source is unlikely, it cannot be excluded. Comparison of the accessory genomes, however, showed an additional 300 kb DNA in A54560.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

Salmonella enterica serotype Typhimurium is one of the most common causes of bloodstream infection in sub-Saharan Africa... Epidemics of iNTS disease in sub-Saharan Africa have been associated with a novel genotype of S. enterica ser... Typhimurium of multilocus sequence type (ST) 313 that is rarely seen outside the region and is associated with multidrug resistance (MDR) to chloramphenicol, cotrimoxazole, and ampicillin... Typhimurium bloodstream infection with additional resistance to ceftriaxone and ciprofloxacin... In the absence of a locally available effective antimicrobial drug, she was treated with ceftriaxone, gentamicin, and high-dose ciprofloxacin but died shortly thereafter... Typhimurium strains A54285 (initial presentation) and A54560 (recurrence); both isolates were resistant to ampicillin, chloramphenicol, and cotrimoxazole, but A54560 exhibited additional resistance to ceftriaxone, ciprofloxacin, and tetracycline... Plasmid extraction and gel electrophoresis of genomic DNA identified a plasmid migrating in the gel to a position approximately equivalent to 120 kb, the size of ST313 virulence plasmid pSLT-BT in both strains, but no 300-kb plasmid was visualized in the ceftriaxone- and ciprofloxacin-resistant strain (A54560, data not shown), possibly because of the difficulty large plasmids have entering standard 1% agarose gels... We identified 331 predicted coding sequences, including 109 genes required for replication and transfer and 61 genes predicted to be associated with metabolism, membranes, virulence, antimicrobial resistance, and a toxin/antitoxin addiction system... We found an additional 160 predicted, hypothetical genes... The spread of mobile genetic elements that confer antimicrobial resistance among gram-negative organisms is of considerable concern... Wide dissemination of this strain or the IncHI2 (pSTm-BTCR) plasmid among other salmonellae in sub-Saharan Africa would rapidly render iNTS effectively untreatable with currently available antibacterial drugs... Detailed methods and plasmid map of pSTm-BTCR.

Show MeSH