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Severe fever with thrombocytopenia syndrome virus, South Korea, 2013.

Park SW, Han MG, Yun SM, Park C, Lee WJ, Ryou J - Emerging Infect. Dis. (2014)

Bottom Line: During 2013, severe fever with thrombocytopenia syndrome was diagnosed in 35 persons in South Korea.Environmental temperature probably affected the monthly and regional distribution of case-patients within the country.Phylogenetic analysis indicated that the isolates from Korea were closely related to isolates from China and Japan.

View Article: PubMed Central - PubMed

ABSTRACT
During 2013, severe fever with thrombocytopenia syndrome was diagnosed in 35 persons in South Korea. Environmental temperature probably affected the monthly and regional distribution of case-patients within the country. Phylogenetic analysis indicated that the isolates from Korea were closely related to isolates from China and Japan.

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Isolation of severe fever with thrombocytopenia syndrome virus (SFTSV) from case-patients, South Korea, 2013. A, B) Indirect immunofluorescent features of Vero E6 cells primed with SFTSV N protein monoclonal antibody and reacted with fluoresce in isothiocyanateconjugated anti-mouse IgG. B) Transmission electron microscopy image of Vero E6 cells infected with SFTSV. Scale bar indicates 500 nm.
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Figure 1: Isolation of severe fever with thrombocytopenia syndrome virus (SFTSV) from case-patients, South Korea, 2013. A, B) Indirect immunofluorescent features of Vero E6 cells primed with SFTSV N protein monoclonal antibody and reacted with fluoresce in isothiocyanateconjugated anti-mouse IgG. B) Transmission electron microscopy image of Vero E6 cells infected with SFTSV. Scale bar indicates 500 nm.

Mentions: To isolate SFTSV, we inoculated subconfluent monolayers of Vero E6 cells with the RT-PCR–positive serum. After the monolayers underwent 3 blind passages in new monolayers of Vero E6 cells (8), we examined the Vero E6 cells for SFTSV by RT-PCR. We considered the virus to be isolated when the specific genes were amplified by RT-PCR. The viruses did not cause cytopathic effects in Vero E6 cells during isolation. Isolation of SFTSV also was confirmed by indirect immunofluorescent assay (IFA) (Figure 1, panels A,B) and electron microscopy (Figure 1, panel C). For IFA, Vero E6 cells infected with SFTSV were incubated at 37°C in a CO2 incubator. Cells were harvested, inoculated, and fixed with acetone on Teflon-coated well slides. IFA was conducted by using a monoclonal SFTSV nucleocapsid protein (N) antibody (manufactured in our laboratory) as the primary antibody. N proteins of SFTSV were distributed throughout the cytoplasm (Figure 1, panels A,B). By electron microscopy, Vero E6 cells infected with the SFTSV Korea isolate KAJJH showed bunyavirus-like particles, 80–100 nm in diameter, located in cytoplasmic vacuoles, presumably in the Golgi apparatus (Figure 1, panel C).


Severe fever with thrombocytopenia syndrome virus, South Korea, 2013.

Park SW, Han MG, Yun SM, Park C, Lee WJ, Ryou J - Emerging Infect. Dis. (2014)

Isolation of severe fever with thrombocytopenia syndrome virus (SFTSV) from case-patients, South Korea, 2013. A, B) Indirect immunofluorescent features of Vero E6 cells primed with SFTSV N protein monoclonal antibody and reacted with fluoresce in isothiocyanateconjugated anti-mouse IgG. B) Transmission electron microscopy image of Vero E6 cells infected with SFTSV. Scale bar indicates 500 nm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4214315&req=5

Figure 1: Isolation of severe fever with thrombocytopenia syndrome virus (SFTSV) from case-patients, South Korea, 2013. A, B) Indirect immunofluorescent features of Vero E6 cells primed with SFTSV N protein monoclonal antibody and reacted with fluoresce in isothiocyanateconjugated anti-mouse IgG. B) Transmission electron microscopy image of Vero E6 cells infected with SFTSV. Scale bar indicates 500 nm.
Mentions: To isolate SFTSV, we inoculated subconfluent monolayers of Vero E6 cells with the RT-PCR–positive serum. After the monolayers underwent 3 blind passages in new monolayers of Vero E6 cells (8), we examined the Vero E6 cells for SFTSV by RT-PCR. We considered the virus to be isolated when the specific genes were amplified by RT-PCR. The viruses did not cause cytopathic effects in Vero E6 cells during isolation. Isolation of SFTSV also was confirmed by indirect immunofluorescent assay (IFA) (Figure 1, panels A,B) and electron microscopy (Figure 1, panel C). For IFA, Vero E6 cells infected with SFTSV were incubated at 37°C in a CO2 incubator. Cells were harvested, inoculated, and fixed with acetone on Teflon-coated well slides. IFA was conducted by using a monoclonal SFTSV nucleocapsid protein (N) antibody (manufactured in our laboratory) as the primary antibody. N proteins of SFTSV were distributed throughout the cytoplasm (Figure 1, panels A,B). By electron microscopy, Vero E6 cells infected with the SFTSV Korea isolate KAJJH showed bunyavirus-like particles, 80–100 nm in diameter, located in cytoplasmic vacuoles, presumably in the Golgi apparatus (Figure 1, panel C).

Bottom Line: During 2013, severe fever with thrombocytopenia syndrome was diagnosed in 35 persons in South Korea.Environmental temperature probably affected the monthly and regional distribution of case-patients within the country.Phylogenetic analysis indicated that the isolates from Korea were closely related to isolates from China and Japan.

View Article: PubMed Central - PubMed

ABSTRACT
During 2013, severe fever with thrombocytopenia syndrome was diagnosed in 35 persons in South Korea. Environmental temperature probably affected the monthly and regional distribution of case-patients within the country. Phylogenetic analysis indicated that the isolates from Korea were closely related to isolates from China and Japan.

Show MeSH
Related in: MedlinePlus