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Protective effects of baicalin on decidua cells of LPS-induced mice abortion.

Wang X, Zhao Y, Zhong X - J Immunol Res (2014)

Bottom Line: The TNF and progesterone contents in the serum were assayed by ELISA.The results clearly revealed that Baicalin can prevent the injury to decidual cells from LPS dose dependently, TNF was decreased significantly (P < 0.01) compared to LPS group, and there was no effect on the progesterone.These findings suggest that Baicalin has protective effects on the injured decidual cells in the pregnant mice.

View Article: PubMed Central - PubMed

Affiliation: College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.

ABSTRACT
The study was carried out to investigate the protective effects of Baicalin on decidual cells of LPS-induced abortion mice. In the in vitro experiment, the decidual cells were cultured by uterus tissue mass cultivation sampled at day 6 of pregnancy, and gradient concentrations of LPS were used to determine the optimal LPS concentration of the injured decidual cells model. The injured decidual cells were treated with Baicalin (4 μg/mL) to determine the protective role of Baicalin. In the in vivo experiment, lipopolysaccharide (LPS) was injected intravenously via the tail vein to induce abortion at day 6 of pregnancy, and the mice were given different concentrations of Baicalin by oral gavage consecutively at days 7 to 8 of pregnancy. On day 9 of gestation, the mice were sacrificed. The TNF and progesterone contents in the serum were assayed by ELISA. The results clearly revealed that Baicalin can prevent the injury to decidual cells from LPS dose dependently, TNF was decreased significantly (P < 0.01) compared to LPS group, and there was no effect on the progesterone. These findings suggest that Baicalin has protective effects on the injured decidual cells in the pregnant mice.

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TNF in serum. Notes: (A) control group, (B) LPS group, (C) low dose of Baicalin (0.25 mg/mL), (D) middle dose of Baicalin (1.25 mg/mL), and (E) high dose of Baicalin (2.5 mg/mL). Different letters mean significant at 5% level, and same letters mean no significant difference.
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fig5: TNF in serum. Notes: (A) control group, (B) LPS group, (C) low dose of Baicalin (0.25 mg/mL), (D) middle dose of Baicalin (1.25 mg/mL), and (E) high dose of Baicalin (2.5 mg/mL). Different letters mean significant at 5% level, and same letters mean no significant difference.

Mentions: The TNF level was increased compared with the PBS-treated mice in group A (P < 0.01). After Baicalin treatment (groups C, D, and E), the TNF levels were downgraded, and the degree of recovery depended on Baicalin concentrations. A better recovery degree was observed in 1.25 mg/mL and 2.5 mg/mL Baicalin groups (P < 0.01). In relation to progesterone, no significant difference was detected between groups (see Figures 5 and 6).


Protective effects of baicalin on decidua cells of LPS-induced mice abortion.

Wang X, Zhao Y, Zhong X - J Immunol Res (2014)

TNF in serum. Notes: (A) control group, (B) LPS group, (C) low dose of Baicalin (0.25 mg/mL), (D) middle dose of Baicalin (1.25 mg/mL), and (E) high dose of Baicalin (2.5 mg/mL). Different letters mean significant at 5% level, and same letters mean no significant difference.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4214105&req=5

fig5: TNF in serum. Notes: (A) control group, (B) LPS group, (C) low dose of Baicalin (0.25 mg/mL), (D) middle dose of Baicalin (1.25 mg/mL), and (E) high dose of Baicalin (2.5 mg/mL). Different letters mean significant at 5% level, and same letters mean no significant difference.
Mentions: The TNF level was increased compared with the PBS-treated mice in group A (P < 0.01). After Baicalin treatment (groups C, D, and E), the TNF levels were downgraded, and the degree of recovery depended on Baicalin concentrations. A better recovery degree was observed in 1.25 mg/mL and 2.5 mg/mL Baicalin groups (P < 0.01). In relation to progesterone, no significant difference was detected between groups (see Figures 5 and 6).

Bottom Line: The TNF and progesterone contents in the serum were assayed by ELISA.The results clearly revealed that Baicalin can prevent the injury to decidual cells from LPS dose dependently, TNF was decreased significantly (P < 0.01) compared to LPS group, and there was no effect on the progesterone.These findings suggest that Baicalin has protective effects on the injured decidual cells in the pregnant mice.

View Article: PubMed Central - PubMed

Affiliation: College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China.

ABSTRACT
The study was carried out to investigate the protective effects of Baicalin on decidual cells of LPS-induced abortion mice. In the in vitro experiment, the decidual cells were cultured by uterus tissue mass cultivation sampled at day 6 of pregnancy, and gradient concentrations of LPS were used to determine the optimal LPS concentration of the injured decidual cells model. The injured decidual cells were treated with Baicalin (4 μg/mL) to determine the protective role of Baicalin. In the in vivo experiment, lipopolysaccharide (LPS) was injected intravenously via the tail vein to induce abortion at day 6 of pregnancy, and the mice were given different concentrations of Baicalin by oral gavage consecutively at days 7 to 8 of pregnancy. On day 9 of gestation, the mice were sacrificed. The TNF and progesterone contents in the serum were assayed by ELISA. The results clearly revealed that Baicalin can prevent the injury to decidual cells from LPS dose dependently, TNF was decreased significantly (P < 0.01) compared to LPS group, and there was no effect on the progesterone. These findings suggest that Baicalin has protective effects on the injured decidual cells in the pregnant mice.

Show MeSH
Related in: MedlinePlus