Limits...
Influenza A/H1N1 Severe Pneumonia: Novel Morphocytological Findings in Bronchoalveolar Lavage.

Faverio P, Aliberti S, Ezekiel C, Messinesi G, Brenna A, Pesci A - Interdiscip Perspect Infect Dis (2014)

Bottom Line: We present the results of bronchoalveolar lavage (BAL) performed in three patients with severe influenza A/H1N1 pneumonia complicated by acute respiratory distress syndrome (ARDS).Via transmission electron microscopy, these cells were classified as atypical type II pneumocytes and some of them showed cytoplasmic vesicles and inclusions.We concluded that plasmoblastic/plasmocytoid-like type II pneumocytes might represent a morphologic marker of A/H1N1 influenza virus infection as well as reparative cellular activation after diffuse alveolar damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Health Science, University of Milan Bicocca, Clinica Pneumologica, AO San Gerardo, Via Pergolesi 33, 20900 Monza, Italy ; University of Texas Health Science Center at San Antonio, 8300 Floyd Curl Drive, San Antonio, TX 78229, USA.

ABSTRACT
We present the results of bronchoalveolar lavage (BAL) performed in three patients with severe influenza A/H1N1 pneumonia complicated by acute respiratory distress syndrome (ARDS). Light microscopy analysis of BAL cytocentrifugates showed the presence of characteristic large, mononuclear, plasmoblastic/plasmocytoid-like cells never described before. Via transmission electron microscopy, these cells were classified as atypical type II pneumocytes and some of them showed cytoplasmic vesicles and inclusions. We concluded that plasmoblastic/plasmocytoid-like type II pneumocytes might represent a morphologic marker of A/H1N1 influenza virus infection as well as reparative cellular activation after diffuse alveolar damage.

No MeSH data available.


Related in: MedlinePlus

Specimen from bronchoalveolar lavage displays some macrophages, neutrophilic granulocytes, and large plasmoblastic/plasmocytoid-like elements (arrow) with basophilic cytoplasm and paranuclear vacuole (May Grunwald Giemsa, magnification ×400).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4214034&req=5

fig1: Specimen from bronchoalveolar lavage displays some macrophages, neutrophilic granulocytes, and large plasmoblastic/plasmocytoid-like elements (arrow) with basophilic cytoplasm and paranuclear vacuole (May Grunwald Giemsa, magnification ×400).

Mentions: BAL cytocentrifugates analyses are summarized in Table 2. Large, atypical cells with plasmocytoid appearance were observed in all three specimens with similar percentages (8, 9, and 6%, resp.). At LM, they appeared as large, plasmoblastic/plasmocytoid-like cells with eccentric nuclei and paranuclear vacuoles, high ratio nucleus/cytoplasm, and intensely basophilic cytoplasm. No clear viral cytopathic effect was observed (Figure 1). Immunocytochemical analysis on cytocentrifugate showed that these cells were negative for CD20 (B lymphocyte marker) and CD138 (plasma cell marker).


Influenza A/H1N1 Severe Pneumonia: Novel Morphocytological Findings in Bronchoalveolar Lavage.

Faverio P, Aliberti S, Ezekiel C, Messinesi G, Brenna A, Pesci A - Interdiscip Perspect Infect Dis (2014)

Specimen from bronchoalveolar lavage displays some macrophages, neutrophilic granulocytes, and large plasmoblastic/plasmocytoid-like elements (arrow) with basophilic cytoplasm and paranuclear vacuole (May Grunwald Giemsa, magnification ×400).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4214034&req=5

fig1: Specimen from bronchoalveolar lavage displays some macrophages, neutrophilic granulocytes, and large plasmoblastic/plasmocytoid-like elements (arrow) with basophilic cytoplasm and paranuclear vacuole (May Grunwald Giemsa, magnification ×400).
Mentions: BAL cytocentrifugates analyses are summarized in Table 2. Large, atypical cells with plasmocytoid appearance were observed in all three specimens with similar percentages (8, 9, and 6%, resp.). At LM, they appeared as large, plasmoblastic/plasmocytoid-like cells with eccentric nuclei and paranuclear vacuoles, high ratio nucleus/cytoplasm, and intensely basophilic cytoplasm. No clear viral cytopathic effect was observed (Figure 1). Immunocytochemical analysis on cytocentrifugate showed that these cells were negative for CD20 (B lymphocyte marker) and CD138 (plasma cell marker).

Bottom Line: We present the results of bronchoalveolar lavage (BAL) performed in three patients with severe influenza A/H1N1 pneumonia complicated by acute respiratory distress syndrome (ARDS).Via transmission electron microscopy, these cells were classified as atypical type II pneumocytes and some of them showed cytoplasmic vesicles and inclusions.We concluded that plasmoblastic/plasmocytoid-like type II pneumocytes might represent a morphologic marker of A/H1N1 influenza virus infection as well as reparative cellular activation after diffuse alveolar damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Health Science, University of Milan Bicocca, Clinica Pneumologica, AO San Gerardo, Via Pergolesi 33, 20900 Monza, Italy ; University of Texas Health Science Center at San Antonio, 8300 Floyd Curl Drive, San Antonio, TX 78229, USA.

ABSTRACT
We present the results of bronchoalveolar lavage (BAL) performed in three patients with severe influenza A/H1N1 pneumonia complicated by acute respiratory distress syndrome (ARDS). Light microscopy analysis of BAL cytocentrifugates showed the presence of characteristic large, mononuclear, plasmoblastic/plasmocytoid-like cells never described before. Via transmission electron microscopy, these cells were classified as atypical type II pneumocytes and some of them showed cytoplasmic vesicles and inclusions. We concluded that plasmoblastic/plasmocytoid-like type II pneumocytes might represent a morphologic marker of A/H1N1 influenza virus infection as well as reparative cellular activation after diffuse alveolar damage.

No MeSH data available.


Related in: MedlinePlus