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Prevalence of suspected tuberculosis in the Kingdom of Saudi Arabia according to conventional and molecular methods.

Al Watban AZ, Al Salamah AA, El Faki MG - J Family Community Med (2014)

Bottom Line: Of all the techniques, the highest detection rate was by PCR which was 10.46% while ZN stain technique was 6.64%, for MGiT culture it was 8.34%, and for LJ culture it was 7.7%.This study is the first in which different methods have been used for detection in the various regions of Saudi Arabia.Collected data are important not only for patients and physicians but for future epidemiological studies to monitor the spread of MTB infection in Saudi Arabia.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.

ABSTRACT

Background: Although the prevalence of suspected tuberculosis (TB) in the Kingdom of Saudi Arabia remains high, there has been a modest decrease in recent years. In this multi-center cross-sectional study, the prevalence of TB was determined by various techniques with the aim of identifying differences and indicating where there is uniformity in findings.

Materials and methods: A total of 3404 samples were collected from Saudi TB patients from different regions in Saudi Arabia: Riyadh, Dammam, Jeddah, Madinah, Hail, Qassim, Jazan, and Taif. Different techniques including Ziehl-Neelsen (ZN), Mycobacteria growth indicator tube (MGiT), Lowenstein-Jensen (LJ), and polymerase chain reaction (PCR) were used to screen for the presence of Mycobacterium tuberculosis (MTB).

Results: ZN stain showed that Riyadh and Dammam had the highest prevalence of MTB with 22% and 21%, respectively, while prevalence was lowest in Jazan and Hail with an incidence of 2% and 3%, respectively. MGiT culture showed that Riyadh, Dammam, and Jeddah had the highest prevalence with a rate of 26%, 22%, and 22%, respectively. LJ culture showed the highest prevalence in Riyadh and Dammam with 22% and 21%, respectively. Of all the techniques, the highest detection rate was by PCR which was 10.46% while ZN stain technique was 6.64%, for MGiT culture it was 8.34%, and for LJ culture it was 7.7%.

Conclusion: This study is the first in which different methods have been used for detection in the various regions of Saudi Arabia. Collected data are important not only for patients and physicians but for future epidemiological studies to monitor the spread of MTB infection in Saudi Arabia.

No MeSH data available.


Related in: MedlinePlus

Mapping of Mycobacterium tuberculosis prevalence rate percent in Saudi Arabia
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Figure 1: Mapping of Mycobacterium tuberculosis prevalence rate percent in Saudi Arabia

Mentions: Table 1 shows the prevalence of suspected TB in the different regions. Differences in prevalence were apparent according to the different methods, with restriction fragment length polymorphism (RFLP) showing the lowest number of TB cases of all the methods. The ZN stain, LJ culture, MGiT culture, and PCR methods indicated that the prevalence of TB was highest in the regions of Riyadh and Dammam [Table 1]. However, by the RFLP method the prevalence of TB was highest in Jeddah and Dammam. Figure 1 shows the mapping of TB in Saudi Arabia. The PCR showed a higher detection rate than all other techniques as indicated here. Using the ZN stain, it was 6.64%, with MGiT culture it was 8.34%, 7.7% with LJ culture and 10.46% with PCR technique.


Prevalence of suspected tuberculosis in the Kingdom of Saudi Arabia according to conventional and molecular methods.

Al Watban AZ, Al Salamah AA, El Faki MG - J Family Community Med (2014)

Mapping of Mycobacterium tuberculosis prevalence rate percent in Saudi Arabia
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4214008&req=5

Figure 1: Mapping of Mycobacterium tuberculosis prevalence rate percent in Saudi Arabia
Mentions: Table 1 shows the prevalence of suspected TB in the different regions. Differences in prevalence were apparent according to the different methods, with restriction fragment length polymorphism (RFLP) showing the lowest number of TB cases of all the methods. The ZN stain, LJ culture, MGiT culture, and PCR methods indicated that the prevalence of TB was highest in the regions of Riyadh and Dammam [Table 1]. However, by the RFLP method the prevalence of TB was highest in Jeddah and Dammam. Figure 1 shows the mapping of TB in Saudi Arabia. The PCR showed a higher detection rate than all other techniques as indicated here. Using the ZN stain, it was 6.64%, with MGiT culture it was 8.34%, 7.7% with LJ culture and 10.46% with PCR technique.

Bottom Line: Of all the techniques, the highest detection rate was by PCR which was 10.46% while ZN stain technique was 6.64%, for MGiT culture it was 8.34%, and for LJ culture it was 7.7%.This study is the first in which different methods have been used for detection in the various regions of Saudi Arabia.Collected data are important not only for patients and physicians but for future epidemiological studies to monitor the spread of MTB infection in Saudi Arabia.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.

ABSTRACT

Background: Although the prevalence of suspected tuberculosis (TB) in the Kingdom of Saudi Arabia remains high, there has been a modest decrease in recent years. In this multi-center cross-sectional study, the prevalence of TB was determined by various techniques with the aim of identifying differences and indicating where there is uniformity in findings.

Materials and methods: A total of 3404 samples were collected from Saudi TB patients from different regions in Saudi Arabia: Riyadh, Dammam, Jeddah, Madinah, Hail, Qassim, Jazan, and Taif. Different techniques including Ziehl-Neelsen (ZN), Mycobacteria growth indicator tube (MGiT), Lowenstein-Jensen (LJ), and polymerase chain reaction (PCR) were used to screen for the presence of Mycobacterium tuberculosis (MTB).

Results: ZN stain showed that Riyadh and Dammam had the highest prevalence of MTB with 22% and 21%, respectively, while prevalence was lowest in Jazan and Hail with an incidence of 2% and 3%, respectively. MGiT culture showed that Riyadh, Dammam, and Jeddah had the highest prevalence with a rate of 26%, 22%, and 22%, respectively. LJ culture showed the highest prevalence in Riyadh and Dammam with 22% and 21%, respectively. Of all the techniques, the highest detection rate was by PCR which was 10.46% while ZN stain technique was 6.64%, for MGiT culture it was 8.34%, and for LJ culture it was 7.7%.

Conclusion: This study is the first in which different methods have been used for detection in the various regions of Saudi Arabia. Collected data are important not only for patients and physicians but for future epidemiological studies to monitor the spread of MTB infection in Saudi Arabia.

No MeSH data available.


Related in: MedlinePlus