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Response surface methodology: optimisation of antifungal bioemulsifier from novel Bacillus thuringiensis.

Rajendran D, Venkatachalam P, Ramakrishnan J - ScientificWorldJournal (2014)

Bottom Line: To accentuate the production and as the first step to improve the yield, a central composite design (CCD) was used to study the effect of various factors like minimal salts (1X and 3X), glycerol concentration (2% and 4%), beef extract concentration (1% and 3%), and sunflower oil concentration (2% and 4%) on the production of bioemulsifier molecule and to optimize the conditions to increase the production.The E 24 emulsification index was used as the response variable as the increase in surfactant production was seen to be proportional to increased emulsification.Under optimum conditions (1X concentration of minimal salts (MS), 2.6% glycerol (v/v), 1% beef extract (w/v), and 2% sunflower oil (v/v)) a 65% increase in yield was produced.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, Tamil Nadu 613401, India.

ABSTRACT
An antifungal bioemulsifier compound was produced from a novel strain of Bacillus thuringiensis pak2310. To accentuate the production and as the first step to improve the yield, a central composite design (CCD) was used to study the effect of various factors like minimal salts (1X and 3X), glycerol concentration (2% and 4%), beef extract concentration (1% and 3%), and sunflower oil concentration (2% and 4%) on the production of bioemulsifier molecule and to optimize the conditions to increase the production. The E 24 emulsification index was used as the response variable as the increase in surfactant production was seen to be proportional to increased emulsification. A quadratic equation was employed to express the response variable in terms of the independent variables. Statistical tools like student's t-test, F-test, and ANOVA were employed to identify the important factors and to test the adequacy of the model. Under optimum conditions (1X concentration of minimal salts (MS), 2.6% glycerol (v/v), 1% beef extract (w/v), and 2% sunflower oil (v/v)) a 65% increase in yield was produced.

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Related in: MedlinePlus

Oil spread assay for study of surface activity of different peaks along with standard surfactants and distilled water as control.
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Related In: Results  -  Collection


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fig6: Oil spread assay for study of surface activity of different peaks along with standard surfactants and distilled water as control.

Mentions: After 6 days of fermentation, the end of idiophase, as calculated from initial growth kinetics study for the standardised media, the exogenous bioemulsifier was extracted and purified. Similarly, a Pseudomonas strain has been reported to enter death phase by the end of the 7th day indicating that 6-day fermentation is not too long. Bacillus species are known to produce conjugated peptide surfactants like surfactins, iturins, fengycins, and bacillomycin. The lipid and water soluble, greasy, and pale yellow coloured bioemulsifier was obtained at the end of the triple stage liquid-liquid extraction and the fractions were pooled. The solvent was evaporated to a final crude extract volume of 10 mL from 500 mL at room temperature. The HPLC analysis yielded 3 peaks and the compound corresponding to the first peak eluted from 5 minutes to 9 minutes, constituting 42% of the total area under the chromatogram, was selected for further study as it had high antifungal property (Figure 5) and surface activity than the other two (Figure 6). The total protein estimation by Lowry on crude extract was evaluated to be 235.21 mg L−1 for crude extract before optimisation and 391 mg L−1 after optimisation. Using the area under the peak method, the bioemulsifier yield for optimised production media was 65.91% higher at 164.25 mg L−1 than that obtained with media of MS with 3% (v/v) glycerol and 1% (w/v) beef extract. In a similar experiment, Li et al. [28] have optimised media components to increase the yield of the pure biosurfactant by twofold.


Response surface methodology: optimisation of antifungal bioemulsifier from novel Bacillus thuringiensis.

Rajendran D, Venkatachalam P, Ramakrishnan J - ScientificWorldJournal (2014)

Oil spread assay for study of surface activity of different peaks along with standard surfactants and distilled water as control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4213991&req=5

fig6: Oil spread assay for study of surface activity of different peaks along with standard surfactants and distilled water as control.
Mentions: After 6 days of fermentation, the end of idiophase, as calculated from initial growth kinetics study for the standardised media, the exogenous bioemulsifier was extracted and purified. Similarly, a Pseudomonas strain has been reported to enter death phase by the end of the 7th day indicating that 6-day fermentation is not too long. Bacillus species are known to produce conjugated peptide surfactants like surfactins, iturins, fengycins, and bacillomycin. The lipid and water soluble, greasy, and pale yellow coloured bioemulsifier was obtained at the end of the triple stage liquid-liquid extraction and the fractions were pooled. The solvent was evaporated to a final crude extract volume of 10 mL from 500 mL at room temperature. The HPLC analysis yielded 3 peaks and the compound corresponding to the first peak eluted from 5 minutes to 9 minutes, constituting 42% of the total area under the chromatogram, was selected for further study as it had high antifungal property (Figure 5) and surface activity than the other two (Figure 6). The total protein estimation by Lowry on crude extract was evaluated to be 235.21 mg L−1 for crude extract before optimisation and 391 mg L−1 after optimisation. Using the area under the peak method, the bioemulsifier yield for optimised production media was 65.91% higher at 164.25 mg L−1 than that obtained with media of MS with 3% (v/v) glycerol and 1% (w/v) beef extract. In a similar experiment, Li et al. [28] have optimised media components to increase the yield of the pure biosurfactant by twofold.

Bottom Line: To accentuate the production and as the first step to improve the yield, a central composite design (CCD) was used to study the effect of various factors like minimal salts (1X and 3X), glycerol concentration (2% and 4%), beef extract concentration (1% and 3%), and sunflower oil concentration (2% and 4%) on the production of bioemulsifier molecule and to optimize the conditions to increase the production.The E 24 emulsification index was used as the response variable as the increase in surfactant production was seen to be proportional to increased emulsification.Under optimum conditions (1X concentration of minimal salts (MS), 2.6% glycerol (v/v), 1% beef extract (w/v), and 2% sunflower oil (v/v)) a 65% increase in yield was produced.

View Article: PubMed Central - PubMed

Affiliation: School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, Tamil Nadu 613401, India.

ABSTRACT
An antifungal bioemulsifier compound was produced from a novel strain of Bacillus thuringiensis pak2310. To accentuate the production and as the first step to improve the yield, a central composite design (CCD) was used to study the effect of various factors like minimal salts (1X and 3X), glycerol concentration (2% and 4%), beef extract concentration (1% and 3%), and sunflower oil concentration (2% and 4%) on the production of bioemulsifier molecule and to optimize the conditions to increase the production. The E 24 emulsification index was used as the response variable as the increase in surfactant production was seen to be proportional to increased emulsification. A quadratic equation was employed to express the response variable in terms of the independent variables. Statistical tools like student's t-test, F-test, and ANOVA were employed to identify the important factors and to test the adequacy of the model. Under optimum conditions (1X concentration of minimal salts (MS), 2.6% glycerol (v/v), 1% beef extract (w/v), and 2% sunflower oil (v/v)) a 65% increase in yield was produced.

Show MeSH
Related in: MedlinePlus