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Effect of Azadirachta indica (Neem) and Aloe vera as compared to subantimicrobial dose doxycycline on matrix metalloproteinases (MMP)-2 and MMP-9: An in-vitro study.

Kudalkar MD, Nayak A, Bhat KS, Nayak RN - Ayu (2014)

Bottom Line: The results showed that the activity of MMP-2 and MMP-9 was significantly decreased by the use of doxycycline, Neem and Aloe vera.Doxycycline in the concentration of 300 μg/ml, showed an 82.1% reduction in the MMP-2 and 82.6% reduction in the MMP-9 activity.The present study demonstrated an inhibitory effect of Neem and Aloe vera on MMP-2 and MMP-9, which are involved in the extracellular matrix degradation during periodontitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Maratha Mandal's Nathajirao G Halgekar Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India.

ABSTRACT

Background: A critical outcome of periodontal diseases is degradation of collagen in the periodontal tissues, by enzymes such as Matrix Metallo-Proteinases (MMPs). Doxycycline is known to down-regulate the activity of MMPs. Azadirachta indica (Neem) and Aloe vera are herbs known to have an anti-inflammatory effect. The present study was designed to evaluate the anti-inflammatory effect of Neem and Aloe vera by way of its inhibitory effect on MMP-2 and MMP-9 activity in cases of chronic periodontitis and compare it with doxcycline.

Materials and methods: A total of 30 subjects were enrolled in this study. Gingival tissue samples were obtained from patients diagnosed with the chronic periodontitis. The tissue extracts were treated with the said drug solutions and inhibition of MMP-2 and MMP-9 was analyzed. Enzymatic activity was detected by electrophoresis. The data was subjected to Student's paired t-test.

Results: The results showed that the activity of MMP-2 and MMP-9 was significantly decreased by the use of doxycycline, Neem and Aloe vera. A 53.5% reduction in the MMP-2 and 52.5% reduction in the MMP-9 activity was seen when samples were subjected to Neem treatment at the concentration of 1500 μg/ml. Tissues treated with Aloe vera in the concentration of 2000 μg/ml showed a 20.09% reduction in the MMP-2 and 20.4% reduction in the MMP-9 activity. Doxycycline in the concentration of 300 μg/ml, showed an 82.1% reduction in the MMP-2 and 82.6% reduction in the MMP-9 activity.

Conclusion: The present study demonstrated an inhibitory effect of Neem and Aloe vera on MMP-2 and MMP-9, which are involved in the extracellular matrix degradation during periodontitis.

No MeSH data available.


Related in: MedlinePlus

Pretreatment of gingival tissue extract with doxycycline (+ve c), Neem (test), Aloe vera (test) and distilled water (−ve c)
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Figure 2: Pretreatment of gingival tissue extract with doxycycline (+ve c), Neem (test), Aloe vera (test) and distilled water (−ve c)

Mentions: To compare the inhibition of MMP-2 and MMP-9 activity, 50 μl of gingival tissue extract was preincubated with freshly prepared solution of 50 μl of Neem (1500 μg/ml), 50 μl of Aloe vera (2000 μg/ml) and 50 μl of doxycycline (300 μg/ml) solution separately, for 60 min at room temperature in separate vials. In addition, 50 μl of gingival tissue extract was also incubated with 50 μl of distilled water, which was used as the control [Figure 2].


Effect of Azadirachta indica (Neem) and Aloe vera as compared to subantimicrobial dose doxycycline on matrix metalloproteinases (MMP)-2 and MMP-9: An in-vitro study.

Kudalkar MD, Nayak A, Bhat KS, Nayak RN - Ayu (2014)

Pretreatment of gingival tissue extract with doxycycline (+ve c), Neem (test), Aloe vera (test) and distilled water (−ve c)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4213975&req=5

Figure 2: Pretreatment of gingival tissue extract with doxycycline (+ve c), Neem (test), Aloe vera (test) and distilled water (−ve c)
Mentions: To compare the inhibition of MMP-2 and MMP-9 activity, 50 μl of gingival tissue extract was preincubated with freshly prepared solution of 50 μl of Neem (1500 μg/ml), 50 μl of Aloe vera (2000 μg/ml) and 50 μl of doxycycline (300 μg/ml) solution separately, for 60 min at room temperature in separate vials. In addition, 50 μl of gingival tissue extract was also incubated with 50 μl of distilled water, which was used as the control [Figure 2].

Bottom Line: The results showed that the activity of MMP-2 and MMP-9 was significantly decreased by the use of doxycycline, Neem and Aloe vera.Doxycycline in the concentration of 300 μg/ml, showed an 82.1% reduction in the MMP-2 and 82.6% reduction in the MMP-9 activity.The present study demonstrated an inhibitory effect of Neem and Aloe vera on MMP-2 and MMP-9, which are involved in the extracellular matrix degradation during periodontitis.

View Article: PubMed Central - PubMed

Affiliation: Department of Periodontology, Maratha Mandal's Nathajirao G Halgekar Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India.

ABSTRACT

Background: A critical outcome of periodontal diseases is degradation of collagen in the periodontal tissues, by enzymes such as Matrix Metallo-Proteinases (MMPs). Doxycycline is known to down-regulate the activity of MMPs. Azadirachta indica (Neem) and Aloe vera are herbs known to have an anti-inflammatory effect. The present study was designed to evaluate the anti-inflammatory effect of Neem and Aloe vera by way of its inhibitory effect on MMP-2 and MMP-9 activity in cases of chronic periodontitis and compare it with doxcycline.

Materials and methods: A total of 30 subjects were enrolled in this study. Gingival tissue samples were obtained from patients diagnosed with the chronic periodontitis. The tissue extracts were treated with the said drug solutions and inhibition of MMP-2 and MMP-9 was analyzed. Enzymatic activity was detected by electrophoresis. The data was subjected to Student's paired t-test.

Results: The results showed that the activity of MMP-2 and MMP-9 was significantly decreased by the use of doxycycline, Neem and Aloe vera. A 53.5% reduction in the MMP-2 and 52.5% reduction in the MMP-9 activity was seen when samples were subjected to Neem treatment at the concentration of 1500 μg/ml. Tissues treated with Aloe vera in the concentration of 2000 μg/ml showed a 20.09% reduction in the MMP-2 and 20.4% reduction in the MMP-9 activity. Doxycycline in the concentration of 300 μg/ml, showed an 82.1% reduction in the MMP-2 and 82.6% reduction in the MMP-9 activity.

Conclusion: The present study demonstrated an inhibitory effect of Neem and Aloe vera on MMP-2 and MMP-9, which are involved in the extracellular matrix degradation during periodontitis.

No MeSH data available.


Related in: MedlinePlus