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Ginsenoside-Rp1-induced apolipoprotein A-1 expression in the LoVo human colon cancer cell line.

Kim MY, Yoo BC, Cho JY - J Ginseng Res (2014)

Bottom Line: G-Rp1 markedly upregulated the protein level of apolipoprotein (Apo)-A1 in LoVo, SNU-407, DLD-1, SNU-638, AGS, KPL-4, and SK-BR-3 cells.The knockdown of Apo-A1 by its small-interfering RNA increased the levels of cleaved poly(ADP-ribose) polymerase and p53 and diminished the proliferation of LoVo cells.These results suggest that G-Rp1 may act as an anticancer agent by strongly inhibiting cell proliferation and enhancing apoptosis through upregulation of Apo-A1.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioinformatics and Life Science, Soongsil University, Seoul, Korea.

ABSTRACT

Background: Ginsenoside Rp1 (G-Rp1) is a novel ginsenoside derived from ginsenoside Rk1. This compound was reported to have anticancer, anti-platelet, and anti-inflammatory activities. In this study, we examined the molecular target of the antiproliferative and proapoptotic activities of G-Rp1.

Methods: To examine the effects of G-Rp1, cell proliferation assays, propidium iodine staining, proteomic analysis by two-dimensional gel electrophoresis, immunoblotting analysis, and a knockdown strategy were used.

Results: G-Rp1 dose-dependently suppressed the proliferation of colorectal cancer LoVo cells and increased their apoptosis. G-Rp1 markedly upregulated the protein level of apolipoprotein (Apo)-A1 in LoVo, SNU-407, DLD-1, SNU-638, AGS, KPL-4, and SK-BR-3 cells. The knockdown of Apo-A1 by its small-interfering RNA increased the levels of cleaved poly(ADP-ribose) polymerase and p53 and diminished the proliferation of LoVo cells.

Conclusion: These results suggest that G-Rp1 may act as an anticancer agent by strongly inhibiting cell proliferation and enhancing apoptosis through upregulation of Apo-A1.

No MeSH data available.


Related in: MedlinePlus

Schematic diagram of ginsenoside-Rp1 action.
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fig6: Schematic diagram of ginsenoside-Rp1 action.

Mentions: In summary, we have demonstrated that G-Rp1 is capable of suppressing the proliferation of colorectal cancer cells and enhancing their apoptosis via enhanced levels of Apo-A1. The protein levels of c-PARP and p53 were enhanced under siApo-A1 treatment, therefore, the Apo-A1-mediated anticancer effect of G-Rp1 might be linked to the functional involvement of these proteins, as summarized in Fig. 6. Future studies will examine the exact molecular mechanism of Apo-A1-dependent G-Rp1 pharmacology in terms of its differentiation-inducing activities.


Ginsenoside-Rp1-induced apolipoprotein A-1 expression in the LoVo human colon cancer cell line.

Kim MY, Yoo BC, Cho JY - J Ginseng Res (2014)

Schematic diagram of ginsenoside-Rp1 action.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4213851&req=5

fig6: Schematic diagram of ginsenoside-Rp1 action.
Mentions: In summary, we have demonstrated that G-Rp1 is capable of suppressing the proliferation of colorectal cancer cells and enhancing their apoptosis via enhanced levels of Apo-A1. The protein levels of c-PARP and p53 were enhanced under siApo-A1 treatment, therefore, the Apo-A1-mediated anticancer effect of G-Rp1 might be linked to the functional involvement of these proteins, as summarized in Fig. 6. Future studies will examine the exact molecular mechanism of Apo-A1-dependent G-Rp1 pharmacology in terms of its differentiation-inducing activities.

Bottom Line: G-Rp1 markedly upregulated the protein level of apolipoprotein (Apo)-A1 in LoVo, SNU-407, DLD-1, SNU-638, AGS, KPL-4, and SK-BR-3 cells.The knockdown of Apo-A1 by its small-interfering RNA increased the levels of cleaved poly(ADP-ribose) polymerase and p53 and diminished the proliferation of LoVo cells.These results suggest that G-Rp1 may act as an anticancer agent by strongly inhibiting cell proliferation and enhancing apoptosis through upregulation of Apo-A1.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioinformatics and Life Science, Soongsil University, Seoul, Korea.

ABSTRACT

Background: Ginsenoside Rp1 (G-Rp1) is a novel ginsenoside derived from ginsenoside Rk1. This compound was reported to have anticancer, anti-platelet, and anti-inflammatory activities. In this study, we examined the molecular target of the antiproliferative and proapoptotic activities of G-Rp1.

Methods: To examine the effects of G-Rp1, cell proliferation assays, propidium iodine staining, proteomic analysis by two-dimensional gel electrophoresis, immunoblotting analysis, and a knockdown strategy were used.

Results: G-Rp1 dose-dependently suppressed the proliferation of colorectal cancer LoVo cells and increased their apoptosis. G-Rp1 markedly upregulated the protein level of apolipoprotein (Apo)-A1 in LoVo, SNU-407, DLD-1, SNU-638, AGS, KPL-4, and SK-BR-3 cells. The knockdown of Apo-A1 by its small-interfering RNA increased the levels of cleaved poly(ADP-ribose) polymerase and p53 and diminished the proliferation of LoVo cells.

Conclusion: These results suggest that G-Rp1 may act as an anticancer agent by strongly inhibiting cell proliferation and enhancing apoptosis through upregulation of Apo-A1.

No MeSH data available.


Related in: MedlinePlus