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Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex.

Choi HS, Kim SY, Park Y, Jung EY, Suh HJ - J Ginseng Res (2014)

Bottom Line: Rapidase treatment significantly elevated total ginsenoside contents compared with the control (p < 0.05).This result showed that Rapidase induced the conversion of ginsenoside glycosides to aglycones.Meanwhile, Cytolase PCL5 and Econase treatments led to a significant increase of uronic acid (acidic polysaccharide) level.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Korea University, Seoul, Korea.

ABSTRACT

Background: In this study, we examined the effects of various enzymes on chemical conversions of ginsenosides in ginseng extract prepared by amylases.

Methods: Rapidase, Econase CE, Viscozyme, Ultraflo L, and Cytolase PCL5 were used for secondary enzymatic hydrolysis after amylase treatment of ginseng extract, and ginsenoside contents, skin permeability, and chemical compositions including total sugar, acidic polysaccharide, and polyphenols were determined on the hydrolyzed ginseng extract.

Results: Rapidase treatment significantly elevated total ginsenoside contents compared with the control (p < 0.05). In particular, deglycosylated ginsenosides including Rg3, which are known as bioactive compounds, were significantly increased after Rapidase treatment (p < 0.05). The Rapidase-treated group also increased the skin permeability of polyphenols compared with the control, showing the highest level of total sugar content among the enzyme treatment groups.

Conclusion: This result showed that Rapidase induced the conversion of ginsenoside glycosides to aglycones. Meanwhile, Cytolase PCL5 and Econase treatments led to a significant increase of uronic acid (acidic polysaccharide) level. Taken together, our data showed that the treatments of enzymes including Rapidase are useful for the conversion and increase of ginsenosides in ginseng extracts or products.

No MeSH data available.


Polyphenol contents of red ginseng extracts treated with various enzymes. The control is red ginseng extract prepared with Spezyme prime and Optidex L-400. Values are mean ± standard deviation (n = 3). Means with different letters are significantly different at p < 0.05 by Duncan's multiple range tests.
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fig3: Polyphenol contents of red ginseng extracts treated with various enzymes. The control is red ginseng extract prepared with Spezyme prime and Optidex L-400. Values are mean ± standard deviation (n = 3). Means with different letters are significantly different at p < 0.05 by Duncan's multiple range tests.

Mentions: As shown in Fig. 3, sequential enzyme treatment did not affect the content of polyphenols, showing a similar level to the control.


Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex.

Choi HS, Kim SY, Park Y, Jung EY, Suh HJ - J Ginseng Res (2014)

Polyphenol contents of red ginseng extracts treated with various enzymes. The control is red ginseng extract prepared with Spezyme prime and Optidex L-400. Values are mean ± standard deviation (n = 3). Means with different letters are significantly different at p < 0.05 by Duncan's multiple range tests.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4213822&req=5

fig3: Polyphenol contents of red ginseng extracts treated with various enzymes. The control is red ginseng extract prepared with Spezyme prime and Optidex L-400. Values are mean ± standard deviation (n = 3). Means with different letters are significantly different at p < 0.05 by Duncan's multiple range tests.
Mentions: As shown in Fig. 3, sequential enzyme treatment did not affect the content of polyphenols, showing a similar level to the control.

Bottom Line: Rapidase treatment significantly elevated total ginsenoside contents compared with the control (p < 0.05).This result showed that Rapidase induced the conversion of ginsenoside glycosides to aglycones.Meanwhile, Cytolase PCL5 and Econase treatments led to a significant increase of uronic acid (acidic polysaccharide) level.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition, Korea University, Seoul, Korea.

ABSTRACT

Background: In this study, we examined the effects of various enzymes on chemical conversions of ginsenosides in ginseng extract prepared by amylases.

Methods: Rapidase, Econase CE, Viscozyme, Ultraflo L, and Cytolase PCL5 were used for secondary enzymatic hydrolysis after amylase treatment of ginseng extract, and ginsenoside contents, skin permeability, and chemical compositions including total sugar, acidic polysaccharide, and polyphenols were determined on the hydrolyzed ginseng extract.

Results: Rapidase treatment significantly elevated total ginsenoside contents compared with the control (p < 0.05). In particular, deglycosylated ginsenosides including Rg3, which are known as bioactive compounds, were significantly increased after Rapidase treatment (p < 0.05). The Rapidase-treated group also increased the skin permeability of polyphenols compared with the control, showing the highest level of total sugar content among the enzyme treatment groups.

Conclusion: This result showed that Rapidase induced the conversion of ginsenoside glycosides to aglycones. Meanwhile, Cytolase PCL5 and Econase treatments led to a significant increase of uronic acid (acidic polysaccharide) level. Taken together, our data showed that the treatments of enzymes including Rapidase are useful for the conversion and increase of ginsenosides in ginseng extracts or products.

No MeSH data available.