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Bio-mimetic nanostructure self-assembled from Au@Ag heterogeneous nanorods and phage fusion proteins for targeted tumor optical detection and photothermal therapy.

Wang F, Liu P, Sun L, Li C, Petrenko VA, Liu A - Sci Rep (2014)

Bottom Line: Au@Ag NRs, first being applied for PTT, exhibited excellent stability, cost-effectivity, biocompatibility and tunable NIR absorption.The nanostructure was more efficient than gold nanorods and silver nanotriangle-based photothermal agents and was capable of specifically ablating SW620 cells after 10 min illumination with an 808 nm laser in the light intensity of 4 W/cm(2).The prepared nanostructure would become an ideal reagent for simutaneously targeted optical imaging and PTT of tumor.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy &Bioprocess Technology, and Key Laboratory of Biofuels, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China, and University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.

ABSTRACT
Nanomaterials with near-infrared (NIR) absorption have been widely studied in cancer detection and photothermal therapy (PTT), while it remains a great challenge in targeting tumor efficiently with minimal side effects. Herein we report a novel multifunctional phage-mimetic nanostructure, which was prepared by layer-by-layer self-assembly of Au@Ag heterogenous nanorods (NRs) with rhodamine 6G, and specific pVIII fusion proteins. Au@Ag NRs, first being applied for PTT, exhibited excellent stability, cost-effectivity, biocompatibility and tunable NIR absorption. The fusion proteins were isolated from phage DDAGNRQP specifically selected from f8/8 landscape phage library against colorectal cancer cells in a high-throughput way. Considering the definite charge distribution and low molecular weight, phage fusion proteins were assembled on the negatively charged NR core by electrostatic interactions, exposing the N-terminus fused with DDAGNRQP peptide on the surface. The fluorescent images showed that assembled phage fusion proteins can direct the nanostructure into cancer cells. The nanostructure was more efficient than gold nanorods and silver nanotriangle-based photothermal agents and was capable of specifically ablating SW620 cells after 10 min illumination with an 808 nm laser in the light intensity of 4 W/cm(2). The prepared nanostructure would become an ideal reagent for simutaneously targeted optical imaging and PTT of tumor.

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Related in: MedlinePlus

The cell viability of HepG2, HEK293T and SW620 cells varying with irradiation time. The selective PTT was tested with an 808 nm laser (4 W/cm2) after the cells incubation with 50 μg/ml PMHNRs for 6 h.(*p < 0.05, mean ± standard deviation, n = 3).
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f8: The cell viability of HepG2, HEK293T and SW620 cells varying with irradiation time. The selective PTT was tested with an 808 nm laser (4 W/cm2) after the cells incubation with 50 μg/ml PMHNRs for 6 h.(*p < 0.05, mean ± standard deviation, n = 3).

Mentions: All cells were exposed to 808 nm laser for different time in the light intensity of 4 W/cm2 after incubation with PMHNRs for 6 h. Control cells had the viability above 80%, and even if the irradiation time was as long as 30 min, cells were slightly influenced (Fig. 8). HepG2 cells died faster than HEK293T cells, probably because cancer cells are more sensitive to high temperature than normal cells or PMHNRs were internalized into HepG2 cells at slightly higher level that into HEK293T cells. As shown in Fig. 8, a large number of SW620 cells died after 10 min constant illumination. It was supposed that sufficient amount of PMHNRs were endocytosed to generate enough heat to ablate SW620 cells under constant light irradiation. Our results indicated that PMHNRs assembled with DDAGNRQP-fused pVIII coat proteins can specifically interact with the target and effectively generate hyperthermia to ablate SW620 cells at a lower irradiation power.


Bio-mimetic nanostructure self-assembled from Au@Ag heterogeneous nanorods and phage fusion proteins for targeted tumor optical detection and photothermal therapy.

Wang F, Liu P, Sun L, Li C, Petrenko VA, Liu A - Sci Rep (2014)

The cell viability of HepG2, HEK293T and SW620 cells varying with irradiation time. The selective PTT was tested with an 808 nm laser (4 W/cm2) after the cells incubation with 50 μg/ml PMHNRs for 6 h.(*p < 0.05, mean ± standard deviation, n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4210868&req=5

f8: The cell viability of HepG2, HEK293T and SW620 cells varying with irradiation time. The selective PTT was tested with an 808 nm laser (4 W/cm2) after the cells incubation with 50 μg/ml PMHNRs for 6 h.(*p < 0.05, mean ± standard deviation, n = 3).
Mentions: All cells were exposed to 808 nm laser for different time in the light intensity of 4 W/cm2 after incubation with PMHNRs for 6 h. Control cells had the viability above 80%, and even if the irradiation time was as long as 30 min, cells were slightly influenced (Fig. 8). HepG2 cells died faster than HEK293T cells, probably because cancer cells are more sensitive to high temperature than normal cells or PMHNRs were internalized into HepG2 cells at slightly higher level that into HEK293T cells. As shown in Fig. 8, a large number of SW620 cells died after 10 min constant illumination. It was supposed that sufficient amount of PMHNRs were endocytosed to generate enough heat to ablate SW620 cells under constant light irradiation. Our results indicated that PMHNRs assembled with DDAGNRQP-fused pVIII coat proteins can specifically interact with the target and effectively generate hyperthermia to ablate SW620 cells at a lower irradiation power.

Bottom Line: Au@Ag NRs, first being applied for PTT, exhibited excellent stability, cost-effectivity, biocompatibility and tunable NIR absorption.The nanostructure was more efficient than gold nanorods and silver nanotriangle-based photothermal agents and was capable of specifically ablating SW620 cells after 10 min illumination with an 808 nm laser in the light intensity of 4 W/cm(2).The prepared nanostructure would become an ideal reagent for simutaneously targeted optical imaging and PTT of tumor.

View Article: PubMed Central - PubMed

Affiliation: Laboratory for Biosensing, Qingdao Institute of Bioenergy &Bioprocess Technology, and Key Laboratory of Biofuels, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China, and University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.

ABSTRACT
Nanomaterials with near-infrared (NIR) absorption have been widely studied in cancer detection and photothermal therapy (PTT), while it remains a great challenge in targeting tumor efficiently with minimal side effects. Herein we report a novel multifunctional phage-mimetic nanostructure, which was prepared by layer-by-layer self-assembly of Au@Ag heterogenous nanorods (NRs) with rhodamine 6G, and specific pVIII fusion proteins. Au@Ag NRs, first being applied for PTT, exhibited excellent stability, cost-effectivity, biocompatibility and tunable NIR absorption. The fusion proteins were isolated from phage DDAGNRQP specifically selected from f8/8 landscape phage library against colorectal cancer cells in a high-throughput way. Considering the definite charge distribution and low molecular weight, phage fusion proteins were assembled on the negatively charged NR core by electrostatic interactions, exposing the N-terminus fused with DDAGNRQP peptide on the surface. The fluorescent images showed that assembled phage fusion proteins can direct the nanostructure into cancer cells. The nanostructure was more efficient than gold nanorods and silver nanotriangle-based photothermal agents and was capable of specifically ablating SW620 cells after 10 min illumination with an 808 nm laser in the light intensity of 4 W/cm(2). The prepared nanostructure would become an ideal reagent for simutaneously targeted optical imaging and PTT of tumor.

Show MeSH
Related in: MedlinePlus