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Evaluation of the BD Vacutainer(®) RST blood collection tube for routine chemistry analytes: clinical significance of differences and stability study.

Kocijancic M, Cargonja J, Delic-Knezevic A - Biochem Med (Zagreb) (2014)

Bottom Line: There was a statistically significant difference between the RST and SST II tubes for glucose, calcium and LD (P < 0.001).Differences for glucose and LD were also clinically significant.Most results (except LD and glucose) from RST are comparable with those from SST.

View Article: PubMed Central - PubMed

Affiliation: Medical biochemistry laboratory of Primorsko-goranska county health care-Rijeka, Rijeka, Croatia.

ABSTRACT

Introduction: Preanalytical variables account for most of laboratory errors. There is a wide range of factors that affect the reliability of laboratory report. Most convenient sample type for routine laboratory analysis is serum. BD Vacutainer(®) Rapid Serum Tube (RST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) blood collection tube provides rapid clotting time allowing fast serum separation. Our aim was to evaluate the comparability of routine chemistry parameters in BD Vacutainer(®) RST blood collection tube in reference with the BD Vacutainer(®) Serum Separating Tubes II Advance Tube (SST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).

Materials and methods: Blood specimens were collected from 90 participants for evaluation on its results, clotting time and stability study of six routine biochemistry parameters: glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), lactate dehidrogenase (LD) and potassium (K) measured with Olympus AU2700 analyzer (Beckman Coulter, Tokyo, Japan). The significance of the differences between samples was assessed by paired t-test or Wilcoxon Matched-Pairs Rank test after checking for normality.

Results: Clotting process was significantly shorter in the RSTs compared to SSTs (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II tubes for glucose, calcium and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C.

Conclusions: Most results (except LD and glucose) from RST are comparable with those from SST. In addition, RST tube provides shorter clotting time.

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Related in: MedlinePlus

Passing-Bablok regression analysis.Results comparison between RST and SST tubes for: (a) glucose, (b) aspartate aminotransferase, (c) alanine aminotransferase, (d) calcium, (e) lactate dehidrogenase, (f) potassium.
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f2-biomed-24-3-368: Passing-Bablok regression analysis.Results comparison between RST and SST tubes for: (a) glucose, (b) aspartate aminotransferase, (c) alanine aminotransferase, (d) calcium, (e) lactate dehidrogenase, (f) potassium.

Mentions: The summary statistics results for evaluation of tested RST against reference SST II Advance tubes for six routine chemistry tests results at initial time are shown in table 2. Parallel mean tests results for AST, ALT and potassium for RSTs were in close agreement with that of the reference (SST) tube. There was a statistically significant difference (P < 0.001) between the tubes for glucose, calcium and LD. Difference for glucose and LD was also clinically significant compared with the current desirable allowable bias based on biological variation. Bland-Altman difference plots (Figure 1) showed high mean bias for glucose and LD. Glucose was 3.8% higher in RST tubes than the mean in reference SST II tubes. LD was 5.7 % lower in RST tubes than the mean in reference SST II tubes. Passing-Bablok regression analysis (Figure 2.) showed that for most analytes bias doesn’t exist. However, a constant bias was observed for LD (y= −9.00 (−13.8 to −3.0) + 1.00 (0.96 to 1.02).


Evaluation of the BD Vacutainer(®) RST blood collection tube for routine chemistry analytes: clinical significance of differences and stability study.

Kocijancic M, Cargonja J, Delic-Knezevic A - Biochem Med (Zagreb) (2014)

Passing-Bablok regression analysis.Results comparison between RST and SST tubes for: (a) glucose, (b) aspartate aminotransferase, (c) alanine aminotransferase, (d) calcium, (e) lactate dehidrogenase, (f) potassium.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4210257&req=5

f2-biomed-24-3-368: Passing-Bablok regression analysis.Results comparison between RST and SST tubes for: (a) glucose, (b) aspartate aminotransferase, (c) alanine aminotransferase, (d) calcium, (e) lactate dehidrogenase, (f) potassium.
Mentions: The summary statistics results for evaluation of tested RST against reference SST II Advance tubes for six routine chemistry tests results at initial time are shown in table 2. Parallel mean tests results for AST, ALT and potassium for RSTs were in close agreement with that of the reference (SST) tube. There was a statistically significant difference (P < 0.001) between the tubes for glucose, calcium and LD. Difference for glucose and LD was also clinically significant compared with the current desirable allowable bias based on biological variation. Bland-Altman difference plots (Figure 1) showed high mean bias for glucose and LD. Glucose was 3.8% higher in RST tubes than the mean in reference SST II tubes. LD was 5.7 % lower in RST tubes than the mean in reference SST II tubes. Passing-Bablok regression analysis (Figure 2.) showed that for most analytes bias doesn’t exist. However, a constant bias was observed for LD (y= −9.00 (−13.8 to −3.0) + 1.00 (0.96 to 1.02).

Bottom Line: There was a statistically significant difference between the RST and SST II tubes for glucose, calcium and LD (P < 0.001).Differences for glucose and LD were also clinically significant.Most results (except LD and glucose) from RST are comparable with those from SST.

View Article: PubMed Central - PubMed

Affiliation: Medical biochemistry laboratory of Primorsko-goranska county health care-Rijeka, Rijeka, Croatia.

ABSTRACT

Introduction: Preanalytical variables account for most of laboratory errors. There is a wide range of factors that affect the reliability of laboratory report. Most convenient sample type for routine laboratory analysis is serum. BD Vacutainer(®) Rapid Serum Tube (RST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) blood collection tube provides rapid clotting time allowing fast serum separation. Our aim was to evaluate the comparability of routine chemistry parameters in BD Vacutainer(®) RST blood collection tube in reference with the BD Vacutainer(®) Serum Separating Tubes II Advance Tube (SST) (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).

Materials and methods: Blood specimens were collected from 90 participants for evaluation on its results, clotting time and stability study of six routine biochemistry parameters: glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), lactate dehidrogenase (LD) and potassium (K) measured with Olympus AU2700 analyzer (Beckman Coulter, Tokyo, Japan). The significance of the differences between samples was assessed by paired t-test or Wilcoxon Matched-Pairs Rank test after checking for normality.

Results: Clotting process was significantly shorter in the RSTs compared to SSTs (2.49 min vs. 19.47 min, respectively; P < 0.001). There was a statistically significant difference between the RST and SST II tubes for glucose, calcium and LD (P < 0.001). Differences for glucose and LD were also clinically significant. Analyte stability studies showed that all analytes were stable for 24 h at 4 °C.

Conclusions: Most results (except LD and glucose) from RST are comparable with those from SST. In addition, RST tube provides shorter clotting time.

Show MeSH
Related in: MedlinePlus