Limits...
Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process Optimization.

Vastrad BM, Neelagund SE, Iiger SR, Godbole AM, Kulkarni V - Biochem Res Int (2014)

Bottom Line: Among the eleven variables tested, galactose, ribose, glucose, and pH were found to have significant effect on rifamycin B production.At these optimum production parameters, the maximum yield of rifamycin B obtained experimentally (9.87 g/kgds dry sunflower oil cake) was found to be very close to its predicted value of 10.35 g/kgds dry sunflower oil cake.The mathematical model developed was found to fit greatly with the experimental data of rifamycin B production.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, S.E.T's College of Pharmacy, Dharwad, Karnataka 580002, India.

ABSTRACT
Optimization of various production parameters using response surface methodology (RSM) was performed to assess maximum yield of rifamycin B from Nocardia mediterranei MTCC 14. Plackett-Burman design test was applied to determine the significant effects of various production parameters such as glucose, maltose, ribose, galactose, beef extract, peanut meal, ammonium chloride, ammonium sulphate, barbital, pH, and moisture content on production of rifamycin B. Among the eleven variables tested, galactose, ribose, glucose, and pH were found to have significant effect on rifamycin B production. Optimum levels of the significant variables were decided by using a central composite design. The most appropriate condition for production of rifamycin B was found to be a single step production at galactose (8% w/w), ribose (3% w/w), glucose (9% w/w), and pH (7.0). At these optimum production parameters, the maximum yield of rifamycin B obtained experimentally (9.87 g/kgds dry sunflower oil cake) was found to be very close to its predicted value of 10.35 g/kgds dry sunflower oil cake. The mathematical model developed was found to fit greatly with the experimental data of rifamycin B production.

No MeSH data available.


Surface and contour plot for rifamycin B production at varying concentrations of X3, ribose (RI), and X1, glucose (GL).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4209767&req=5

fig10: Surface and contour plot for rifamycin B production at varying concentrations of X3, ribose (RI), and X1, glucose (GL).

Mentions: Figures 7–12 show the surface and contour plots of rifamycin B produced for each pair of production parameters by keeping the other two production parameters constant at its central level. The effect of galactose and ribose on the production of rifamycin B is shown in Figure 7. The maximum rifamycin B (8.34 g/kgds) was obtained at galactose 8.5% w/w and ribose 3.25% w/w. Further increase in concentration of galactose and ribose leads to increase in production of rifamycin B. Figure 8 indicates that the maximum rifamycin B was produced (5.57 g/kgds) when galactose and glucose were 8% w/w and 9% w/w; further increase in the concentration of galactose leads to increase in production of rifamycin B and further decrease in the concentration of glucose leads to increase in production of rifamycin B. The surface and contour plot of Figure 9 indicates that the maximum rifamycin B (4.80 g/kgds) production occurred at the galactose of 9% w/w and pH of 7. The production of rifamycin B increases with increase in concentration of galactose up to 9% w/w and decrease in pH up to 7 and further increase in the concentration of galactose and further decrease in pH lead to increase in the production of rifamycin B. Figure 10 indicates that the maximum rifamycin B is produced at the concentrations of ribose above 4% w/w and below 3% w/w and glucose above 9% w/w and below 8% w/w. Maximum rifamycin B was produced when concentration of ribose was above 4% w/w and below 3% w/w and pH was 7 (Figure 11). Further decrease in pH leads to acceleration of rifamycin B production. Figure 12 surface and contour plot shows that maximum rifamycin B was produced at concentration of glucose of 9% w/w and pH was 7.


Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process Optimization.

Vastrad BM, Neelagund SE, Iiger SR, Godbole AM, Kulkarni V - Biochem Res Int (2014)

Surface and contour plot for rifamycin B production at varying concentrations of X3, ribose (RI), and X1, glucose (GL).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4209767&req=5

fig10: Surface and contour plot for rifamycin B production at varying concentrations of X3, ribose (RI), and X1, glucose (GL).
Mentions: Figures 7–12 show the surface and contour plots of rifamycin B produced for each pair of production parameters by keeping the other two production parameters constant at its central level. The effect of galactose and ribose on the production of rifamycin B is shown in Figure 7. The maximum rifamycin B (8.34 g/kgds) was obtained at galactose 8.5% w/w and ribose 3.25% w/w. Further increase in concentration of galactose and ribose leads to increase in production of rifamycin B. Figure 8 indicates that the maximum rifamycin B was produced (5.57 g/kgds) when galactose and glucose were 8% w/w and 9% w/w; further increase in the concentration of galactose leads to increase in production of rifamycin B and further decrease in the concentration of glucose leads to increase in production of rifamycin B. The surface and contour plot of Figure 9 indicates that the maximum rifamycin B (4.80 g/kgds) production occurred at the galactose of 9% w/w and pH of 7. The production of rifamycin B increases with increase in concentration of galactose up to 9% w/w and decrease in pH up to 7 and further increase in the concentration of galactose and further decrease in pH lead to increase in the production of rifamycin B. Figure 10 indicates that the maximum rifamycin B is produced at the concentrations of ribose above 4% w/w and below 3% w/w and glucose above 9% w/w and below 8% w/w. Maximum rifamycin B was produced when concentration of ribose was above 4% w/w and below 3% w/w and pH was 7 (Figure 11). Further decrease in pH leads to acceleration of rifamycin B production. Figure 12 surface and contour plot shows that maximum rifamycin B was produced at concentration of glucose of 9% w/w and pH was 7.

Bottom Line: Among the eleven variables tested, galactose, ribose, glucose, and pH were found to have significant effect on rifamycin B production.At these optimum production parameters, the maximum yield of rifamycin B obtained experimentally (9.87 g/kgds dry sunflower oil cake) was found to be very close to its predicted value of 10.35 g/kgds dry sunflower oil cake.The mathematical model developed was found to fit greatly with the experimental data of rifamycin B production.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, S.E.T's College of Pharmacy, Dharwad, Karnataka 580002, India.

ABSTRACT
Optimization of various production parameters using response surface methodology (RSM) was performed to assess maximum yield of rifamycin B from Nocardia mediterranei MTCC 14. Plackett-Burman design test was applied to determine the significant effects of various production parameters such as glucose, maltose, ribose, galactose, beef extract, peanut meal, ammonium chloride, ammonium sulphate, barbital, pH, and moisture content on production of rifamycin B. Among the eleven variables tested, galactose, ribose, glucose, and pH were found to have significant effect on rifamycin B production. Optimum levels of the significant variables were decided by using a central composite design. The most appropriate condition for production of rifamycin B was found to be a single step production at galactose (8% w/w), ribose (3% w/w), glucose (9% w/w), and pH (7.0). At these optimum production parameters, the maximum yield of rifamycin B obtained experimentally (9.87 g/kgds dry sunflower oil cake) was found to be very close to its predicted value of 10.35 g/kgds dry sunflower oil cake. The mathematical model developed was found to fit greatly with the experimental data of rifamycin B production.

No MeSH data available.