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Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process Optimization.

Vastrad BM, Neelagund SE, Iiger SR, Godbole AM, Kulkarni V - Biochem Res Int (2014)

Bottom Line: Optimum levels of the significant variables were decided by using a central composite design.At these optimum production parameters, the maximum yield of rifamycin B obtained experimentally (9.87 g/kgds dry sunflower oil cake) was found to be very close to its predicted value of 10.35 g/kgds dry sunflower oil cake.The mathematical model developed was found to fit greatly with the experimental data of rifamycin B production.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, S.E.T's College of Pharmacy, Dharwad, Karnataka 580002, India.

ABSTRACT
Optimization of various production parameters using response surface methodology (RSM) was performed to assess maximum yield of rifamycin B from Nocardia mediterranei MTCC 14. Plackett-Burman design test was applied to determine the significant effects of various production parameters such as glucose, maltose, ribose, galactose, beef extract, peanut meal, ammonium chloride, ammonium sulphate, barbital, pH, and moisture content on production of rifamycin B. Among the eleven variables tested, galactose, ribose, glucose, and pH were found to have significant effect on rifamycin B production. Optimum levels of the significant variables were decided by using a central composite design. The most appropriate condition for production of rifamycin B was found to be a single step production at galactose (8% w/w), ribose (3% w/w), glucose (9% w/w), and pH (7.0). At these optimum production parameters, the maximum yield of rifamycin B obtained experimentally (9.87 g/kgds dry sunflower oil cake) was found to be very close to its predicted value of 10.35 g/kgds dry sunflower oil cake. The mathematical model developed was found to fit greatly with the experimental data of rifamycin B production.

No MeSH data available.


Related in: MedlinePlus

Effect of various nitrogen sources (7% w/w) on rifamycin B production by Nocardia mediterranei MTCC 14 using sunflower oil cake under solid-state fermentation. Cl: control, PM: peanut meal, Be: beef extract, SM: soybean meal, AS: ammonium sulphate, and AC: ammonium chloride.
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fig2: Effect of various nitrogen sources (7% w/w) on rifamycin B production by Nocardia mediterranei MTCC 14 using sunflower oil cake under solid-state fermentation. Cl: control, PM: peanut meal, Be: beef extract, SM: soybean meal, AS: ammonium sulphate, and AC: ammonium chloride.

Mentions: Rifamycin B production in different microbial strains is reported to be supported by the presence of various organic nitrogen sources such as beef extract [6], soybean meal [19], peanut meal [20], ammonium sulphate [6], and ammonium chloride [6] during solid-state fermentation. Hence, the effect of different nitrogen sources on rifamycin B production by Nocardia mediterranei MTCC 14 was studied at optimized SSF environment. The effect of the supplementation of nitrogen sources was studied by adding various nitrogen sources at a concentration of 7% w/w. Results indicated that they almost completely stimulated the production of rifamycin B at the day eight of incubation. Soybean meal did not affect rifamycin B yield (Figure 2) compared to control (CL). Almost all the nitrogen sources tested increased the rifamycin B production at the day eight of incubation.


Improved Rifamycin B Production by Nocardia mediterranei MTCC 14 under Solid-State Fermentation through Process Optimization.

Vastrad BM, Neelagund SE, Iiger SR, Godbole AM, Kulkarni V - Biochem Res Int (2014)

Effect of various nitrogen sources (7% w/w) on rifamycin B production by Nocardia mediterranei MTCC 14 using sunflower oil cake under solid-state fermentation. Cl: control, PM: peanut meal, Be: beef extract, SM: soybean meal, AS: ammonium sulphate, and AC: ammonium chloride.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4209767&req=5

fig2: Effect of various nitrogen sources (7% w/w) on rifamycin B production by Nocardia mediterranei MTCC 14 using sunflower oil cake under solid-state fermentation. Cl: control, PM: peanut meal, Be: beef extract, SM: soybean meal, AS: ammonium sulphate, and AC: ammonium chloride.
Mentions: Rifamycin B production in different microbial strains is reported to be supported by the presence of various organic nitrogen sources such as beef extract [6], soybean meal [19], peanut meal [20], ammonium sulphate [6], and ammonium chloride [6] during solid-state fermentation. Hence, the effect of different nitrogen sources on rifamycin B production by Nocardia mediterranei MTCC 14 was studied at optimized SSF environment. The effect of the supplementation of nitrogen sources was studied by adding various nitrogen sources at a concentration of 7% w/w. Results indicated that they almost completely stimulated the production of rifamycin B at the day eight of incubation. Soybean meal did not affect rifamycin B yield (Figure 2) compared to control (CL). Almost all the nitrogen sources tested increased the rifamycin B production at the day eight of incubation.

Bottom Line: Optimum levels of the significant variables were decided by using a central composite design.At these optimum production parameters, the maximum yield of rifamycin B obtained experimentally (9.87 g/kgds dry sunflower oil cake) was found to be very close to its predicted value of 10.35 g/kgds dry sunflower oil cake.The mathematical model developed was found to fit greatly with the experimental data of rifamycin B production.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Biotechnology, S.E.T's College of Pharmacy, Dharwad, Karnataka 580002, India.

ABSTRACT
Optimization of various production parameters using response surface methodology (RSM) was performed to assess maximum yield of rifamycin B from Nocardia mediterranei MTCC 14. Plackett-Burman design test was applied to determine the significant effects of various production parameters such as glucose, maltose, ribose, galactose, beef extract, peanut meal, ammonium chloride, ammonium sulphate, barbital, pH, and moisture content on production of rifamycin B. Among the eleven variables tested, galactose, ribose, glucose, and pH were found to have significant effect on rifamycin B production. Optimum levels of the significant variables were decided by using a central composite design. The most appropriate condition for production of rifamycin B was found to be a single step production at galactose (8% w/w), ribose (3% w/w), glucose (9% w/w), and pH (7.0). At these optimum production parameters, the maximum yield of rifamycin B obtained experimentally (9.87 g/kgds dry sunflower oil cake) was found to be very close to its predicted value of 10.35 g/kgds dry sunflower oil cake. The mathematical model developed was found to fit greatly with the experimental data of rifamycin B production.

No MeSH data available.


Related in: MedlinePlus