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The conserved mitochondrial gene distribution in relatives of Turritopsis nutricula, an immortal jellyfish.

Devarapalli P, Kumavath RN, Barh D, Azevedo V - Bioinformation (2014)

Bottom Line: Here, using phylogenetic analysis based on available 16s rRNA gene and protein sequences of Cytochrome oxidase subunit-I (COI or COX1) of T. nutricula, we have predicted the closest relatives of the organism.While we found Nemopsis bachei could be closest organism based on COX1 gene sequence; T. dohrnii may be designated as the closest taxon to T. nutricula based on rRNA.Moreover, we have figured out four species that showed similar root distance based on COX1 protein sequence.

View Article: PubMed Central - PubMed

Affiliation: Department of Genomic Science, Central University of Kerala, Riverside Transit Campus, Opp: Nehru College of Arts and Science, NH 17, Padanakkad, Nileshwer, Kasaragod, Kerala-671328, INDIA ; Genomics & Molecular Medicine Unit, Institute of Genomics and Integrative Biology Council of Scientific and Industrial Research, Mathura Road, New Delhi-110025, INDIA.

ABSTRACT
Turritopsis nutricula (T. nutricula) is the one of the known reported organisms that can revert its life cycle to the polyp stage even after becoming sexually mature, defining itself as the only immortal organism in the animal kingdom. Therefore, the animal is having prime importance in basic biological, aging, and biomedical researches. However, till date, the genome of this organism has not been sequenced and even there is no molecular phylogenetic study to reveal its close relatives. Here, using phylogenetic analysis based on available 16s rRNA gene and protein sequences of Cytochrome oxidase subunit-I (COI or COX1) of T. nutricula, we have predicted the closest relatives of the organism. While we found Nemopsis bachei could be closest organism based on COX1 gene sequence; T. dohrnii may be designated as the closest taxon to T. nutricula based on rRNA. Moreover, we have figured out four species that showed similar root distance based on COX1 protein sequence.

No MeSH data available.


Related in: MedlinePlus

Phylogram representing Turritopsis nutricula and itsCOX1 nt sequence based relatives according to specific rootdistance. Turritopsis nutricula and Nemopsis bachei are emergingfrom the same node.
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Figure 1: Phylogram representing Turritopsis nutricula and itsCOX1 nt sequence based relatives according to specific rootdistance. Turritopsis nutricula and Nemopsis bachei are emergingfrom the same node.

Mentions: Homologous sequence similarity search provided number ofrelated species from which best homologous sequences wereselected according to the cut-off values Table 1 (seesupplementary material). Nemopsis bachei is found to be closestrelative of T. nutricula in COI/COX1 gene based study thatexhibits the shortest root distance of 0.174 (Figure 1 & Figure 2) (Table 1)with a bootstrapping percentage of 88. The 16S rRNA basedanalysis Table 2 (see supplementary material) revealed thatthe immortal jellyfish is genetically close to T. dohrnii [17,18](Root distance: 0.034) followed by T. rubra [17,18] (Rootdistance: 0.099) (Figure 3 & Figure 4) (Table 1)with the bootstrappingpercentages of 100 and 99.In contrast to the nucleotide or genebased analysis, the COI/COX1 protein based analysis Table 3(see supplementary material) showed some different findings.The result demonstrates that T. lata, Amphinema dinema,Eucheilota menoni, and Eutima levuka have similar root distanceof 0.029 (Figure 5 & Figure 6) (Table 1). These species have shown alow bootstrapping percentage of 70 when compared to the geneand 16s rRNA bootstrapping results.


The conserved mitochondrial gene distribution in relatives of Turritopsis nutricula, an immortal jellyfish.

Devarapalli P, Kumavath RN, Barh D, Azevedo V - Bioinformation (2014)

Phylogram representing Turritopsis nutricula and itsCOX1 nt sequence based relatives according to specific rootdistance. Turritopsis nutricula and Nemopsis bachei are emergingfrom the same node.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4209368&req=5

Figure 1: Phylogram representing Turritopsis nutricula and itsCOX1 nt sequence based relatives according to specific rootdistance. Turritopsis nutricula and Nemopsis bachei are emergingfrom the same node.
Mentions: Homologous sequence similarity search provided number ofrelated species from which best homologous sequences wereselected according to the cut-off values Table 1 (seesupplementary material). Nemopsis bachei is found to be closestrelative of T. nutricula in COI/COX1 gene based study thatexhibits the shortest root distance of 0.174 (Figure 1 & Figure 2) (Table 1)with a bootstrapping percentage of 88. The 16S rRNA basedanalysis Table 2 (see supplementary material) revealed thatthe immortal jellyfish is genetically close to T. dohrnii [17,18](Root distance: 0.034) followed by T. rubra [17,18] (Rootdistance: 0.099) (Figure 3 & Figure 4) (Table 1)with the bootstrappingpercentages of 100 and 99.In contrast to the nucleotide or genebased analysis, the COI/COX1 protein based analysis Table 3(see supplementary material) showed some different findings.The result demonstrates that T. lata, Amphinema dinema,Eucheilota menoni, and Eutima levuka have similar root distanceof 0.029 (Figure 5 & Figure 6) (Table 1). These species have shown alow bootstrapping percentage of 70 when compared to the geneand 16s rRNA bootstrapping results.

Bottom Line: Here, using phylogenetic analysis based on available 16s rRNA gene and protein sequences of Cytochrome oxidase subunit-I (COI or COX1) of T. nutricula, we have predicted the closest relatives of the organism.While we found Nemopsis bachei could be closest organism based on COX1 gene sequence; T. dohrnii may be designated as the closest taxon to T. nutricula based on rRNA.Moreover, we have figured out four species that showed similar root distance based on COX1 protein sequence.

View Article: PubMed Central - PubMed

Affiliation: Department of Genomic Science, Central University of Kerala, Riverside Transit Campus, Opp: Nehru College of Arts and Science, NH 17, Padanakkad, Nileshwer, Kasaragod, Kerala-671328, INDIA ; Genomics & Molecular Medicine Unit, Institute of Genomics and Integrative Biology Council of Scientific and Industrial Research, Mathura Road, New Delhi-110025, INDIA.

ABSTRACT
Turritopsis nutricula (T. nutricula) is the one of the known reported organisms that can revert its life cycle to the polyp stage even after becoming sexually mature, defining itself as the only immortal organism in the animal kingdom. Therefore, the animal is having prime importance in basic biological, aging, and biomedical researches. However, till date, the genome of this organism has not been sequenced and even there is no molecular phylogenetic study to reveal its close relatives. Here, using phylogenetic analysis based on available 16s rRNA gene and protein sequences of Cytochrome oxidase subunit-I (COI or COX1) of T. nutricula, we have predicted the closest relatives of the organism. While we found Nemopsis bachei could be closest organism based on COX1 gene sequence; T. dohrnii may be designated as the closest taxon to T. nutricula based on rRNA. Moreover, we have figured out four species that showed similar root distance based on COX1 protein sequence.

No MeSH data available.


Related in: MedlinePlus