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Peptaibols from Trichoderma asperellum TR356 strain isolated from Brazilian soil.

Brito JP, Ramada MH, de Magalhães MT, Silva LP, Ulhoa CJ - Springerplus (2014)

Bottom Line: Liquid medium filtrate was used as the metabolite source.Most of these peptaibols had been previously described in T. viride and T. asperellum marine strains.Future analyses will be necessary to elucidate the three-dimensional structures and their activities against pathogens.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Goiás (ICB II), 74001-970 Goiânia, GO Brasil.

ABSTRACT
The Trichoderma genus consists of a group of free-living filamentous fungi, including species able to act as biological control agents (BCAs) against pathogenic fungi. It is believed that this ability is due to synergy between several mechanisms, including the production of a wide variety of secondary metabolites by these organisms. Among these, we highlight the production of peptaibols, an antibiotic peptide group characterized by the presence of non-proteinogenic amino acids such as α-aminoisobutyrate (Aib), as well as by N-terminal modifications and amino alcohols in the C-terminal region. This study aimed to outline a profile of peptaibol production and to identify secreted peptaibols from the Trichoderma asperellum TR356 strain, described as an efficient BCA against S. sclerotiorum. The fungus was grown on TLE 0.3% glucose medium for 5 days, with agitation at 120 rpm in the dark. Liquid medium filtrate was used as the metabolite source. These extracts were subjected to high performance liquid chromatography (HPLC) and subsequent analysis by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF). The results indicate the production of two classes of peptaibols for this T. asperellum strain. Primary structures of two asperelines (A and E) and five trichotoxins (T5D2, T5E, T5F, T5G and 1717A) have been elucidated. Most of these peptaibols had been previously described in T. viride and T. asperellum marine strains. This is the first report of some of these compounds being produced by a T. asperellum strain from soil. Future analyses will be necessary to elucidate the three-dimensional structures and their activities against pathogens.

No MeSH data available.


Related in: MedlinePlus

Positive-ion MS fromT. asperellumTR356 fraction 7 (a); Fragmentation of ionm/z1726.1 (b).
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Fig6: Positive-ion MS fromT. asperellumTR356 fraction 7 (a); Fragmentation of ionm/z1726.1 (b).

Mentions: In fraction 7, two prominent ions were observed (Figure 6a), but only the m/z 1726.1 [M + Na+] profile was elucidated. On this fragmentation mass spectrum (Figure 6b), we can see part of both series (-y and -b). Again we see the presence of a sodium ion between Aib and proline residues, and in this mass spectrum it was possible to identify the amino acid residues present in the y- series N-terminus, confirming what we find in literature. The analysis of fraction 8 (Figure 7a) was identical to fraction 7, and again only the ion of m/z 1726.2 [M + Na+] was interpreted (Figure 7b). By comparing these sequences with the databases, in both fractions there are peptaibols similar to the trichotoxin A-50 G (T5G), also found in the previous fraction.Figure 6


Peptaibols from Trichoderma asperellum TR356 strain isolated from Brazilian soil.

Brito JP, Ramada MH, de Magalhães MT, Silva LP, Ulhoa CJ - Springerplus (2014)

Positive-ion MS fromT. asperellumTR356 fraction 7 (a); Fragmentation of ionm/z1726.1 (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4209003&req=5

Fig6: Positive-ion MS fromT. asperellumTR356 fraction 7 (a); Fragmentation of ionm/z1726.1 (b).
Mentions: In fraction 7, two prominent ions were observed (Figure 6a), but only the m/z 1726.1 [M + Na+] profile was elucidated. On this fragmentation mass spectrum (Figure 6b), we can see part of both series (-y and -b). Again we see the presence of a sodium ion between Aib and proline residues, and in this mass spectrum it was possible to identify the amino acid residues present in the y- series N-terminus, confirming what we find in literature. The analysis of fraction 8 (Figure 7a) was identical to fraction 7, and again only the ion of m/z 1726.2 [M + Na+] was interpreted (Figure 7b). By comparing these sequences with the databases, in both fractions there are peptaibols similar to the trichotoxin A-50 G (T5G), also found in the previous fraction.Figure 6

Bottom Line: Liquid medium filtrate was used as the metabolite source.Most of these peptaibols had been previously described in T. viride and T. asperellum marine strains.Future analyses will be necessary to elucidate the three-dimensional structures and their activities against pathogens.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Goiás (ICB II), 74001-970 Goiânia, GO Brasil.

ABSTRACT
The Trichoderma genus consists of a group of free-living filamentous fungi, including species able to act as biological control agents (BCAs) against pathogenic fungi. It is believed that this ability is due to synergy between several mechanisms, including the production of a wide variety of secondary metabolites by these organisms. Among these, we highlight the production of peptaibols, an antibiotic peptide group characterized by the presence of non-proteinogenic amino acids such as α-aminoisobutyrate (Aib), as well as by N-terminal modifications and amino alcohols in the C-terminal region. This study aimed to outline a profile of peptaibol production and to identify secreted peptaibols from the Trichoderma asperellum TR356 strain, described as an efficient BCA against S. sclerotiorum. The fungus was grown on TLE 0.3% glucose medium for 5 days, with agitation at 120 rpm in the dark. Liquid medium filtrate was used as the metabolite source. These extracts were subjected to high performance liquid chromatography (HPLC) and subsequent analysis by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF). The results indicate the production of two classes of peptaibols for this T. asperellum strain. Primary structures of two asperelines (A and E) and five trichotoxins (T5D2, T5E, T5F, T5G and 1717A) have been elucidated. Most of these peptaibols had been previously described in T. viride and T. asperellum marine strains. This is the first report of some of these compounds being produced by a T. asperellum strain from soil. Future analyses will be necessary to elucidate the three-dimensional structures and their activities against pathogens.

No MeSH data available.


Related in: MedlinePlus