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An emerging mycoplasma associated with trichomoniasis, vaginal infection and disease.

Fettweis JM, Serrano MG, Huang B, Brooks JP, Glascock AL, Sheth NU, Vaginal Microbiome ConsortiumStrauss JF, Jefferson KK, Buck GA - PLoS ONE (2014)

Bottom Line: Here, we characterize an uncultivated vaginal mycoplasma tightly associated with trichomoniasis that was previously known by its 16S rRNA sequence as "Mnola." In this study, the mycoplasma was found almost exclusively in women infected with the sexually transmitted pathogen Trichomonas vaginalis, but rarely observed in women with no diagnosed disease.The genomes of four strains of this species were reconstructed using metagenome sequencing and assembly of DNA from four discrete mid-vaginal samples, one of which was obtained from a pregnant woman with trichomoniasis who delivered prematurely.Genes encoding proteins with high similarity to potential virulence factors include two collagenases, a hemolysin, an O-sialoglycoprotein endopeptidase and a feoB-type ferrous iron transport system.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America; Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia, United States of America.

ABSTRACT
Humans are colonized by thousands of bacterial species, but it is difficult to assess the metabolic and pathogenic potential of the majority of these because they have yet to be cultured. Here, we characterize an uncultivated vaginal mycoplasma tightly associated with trichomoniasis that was previously known by its 16S rRNA sequence as "Mnola." In this study, the mycoplasma was found almost exclusively in women infected with the sexually transmitted pathogen Trichomonas vaginalis, but rarely observed in women with no diagnosed disease. The genomes of four strains of this species were reconstructed using metagenome sequencing and assembly of DNA from four discrete mid-vaginal samples, one of which was obtained from a pregnant woman with trichomoniasis who delivered prematurely. These bacteria harbor several putative virulence factors and display unique metabolic strategies. Genes encoding proteins with high similarity to potential virulence factors include two collagenases, a hemolysin, an O-sialoglycoprotein endopeptidase and a feoB-type ferrous iron transport system. We propose the name "Candidatus Mycoplasma girerdii" for this potential new pathogen.

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Phylogenetic Tree based on inferred amino acid sequences confirms placement of “Ca. M. girerdii” in the Pneumoniae group.“Ca. M. girerdii” is located within the Pneumoniae group, denoted in green, in a subclade along with the Ureaplasma species, M. iowae and M. penetrans. The tree was inferred using amino acid sequences of 57 orthologs (Tables S4, S5, and S6). Numbers at nodes correspond to the support values from 1,000 bootstrap replicates.
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pone-0110943-g005: Phylogenetic Tree based on inferred amino acid sequences confirms placement of “Ca. M. girerdii” in the Pneumoniae group.“Ca. M. girerdii” is located within the Pneumoniae group, denoted in green, in a subclade along with the Ureaplasma species, M. iowae and M. penetrans. The tree was inferred using amino acid sequences of 57 orthologs (Tables S4, S5, and S6). Numbers at nodes correspond to the support values from 1,000 bootstrap replicates.

Mentions: A phylogenetic analysis using 57 inferred orthologous proteins placed “Ca. M. girerdii” in the Pneumoniae group with M. penetrans and Mycoplasma iowae, relatively distant from M. hominis (Figure 5). This phylogeny is supported by analysis. Analysis of COGs distributed 415 of the “Ca. M. girerdii” genes among functional categories in a pattern similar to that exhibited by other mycoplasma species (Table S1). The full-length “Ca. M. girerdii” 16S rRNA gene from all four genomes and “Mnola” exhibited 100% identity.


An emerging mycoplasma associated with trichomoniasis, vaginal infection and disease.

Fettweis JM, Serrano MG, Huang B, Brooks JP, Glascock AL, Sheth NU, Vaginal Microbiome ConsortiumStrauss JF, Jefferson KK, Buck GA - PLoS ONE (2014)

Phylogenetic Tree based on inferred amino acid sequences confirms placement of “Ca. M. girerdii” in the Pneumoniae group.“Ca. M. girerdii” is located within the Pneumoniae group, denoted in green, in a subclade along with the Ureaplasma species, M. iowae and M. penetrans. The tree was inferred using amino acid sequences of 57 orthologs (Tables S4, S5, and S6). Numbers at nodes correspond to the support values from 1,000 bootstrap replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4206474&req=5

pone-0110943-g005: Phylogenetic Tree based on inferred amino acid sequences confirms placement of “Ca. M. girerdii” in the Pneumoniae group.“Ca. M. girerdii” is located within the Pneumoniae group, denoted in green, in a subclade along with the Ureaplasma species, M. iowae and M. penetrans. The tree was inferred using amino acid sequences of 57 orthologs (Tables S4, S5, and S6). Numbers at nodes correspond to the support values from 1,000 bootstrap replicates.
Mentions: A phylogenetic analysis using 57 inferred orthologous proteins placed “Ca. M. girerdii” in the Pneumoniae group with M. penetrans and Mycoplasma iowae, relatively distant from M. hominis (Figure 5). This phylogeny is supported by analysis. Analysis of COGs distributed 415 of the “Ca. M. girerdii” genes among functional categories in a pattern similar to that exhibited by other mycoplasma species (Table S1). The full-length “Ca. M. girerdii” 16S rRNA gene from all four genomes and “Mnola” exhibited 100% identity.

Bottom Line: Here, we characterize an uncultivated vaginal mycoplasma tightly associated with trichomoniasis that was previously known by its 16S rRNA sequence as "Mnola." In this study, the mycoplasma was found almost exclusively in women infected with the sexually transmitted pathogen Trichomonas vaginalis, but rarely observed in women with no diagnosed disease.The genomes of four strains of this species were reconstructed using metagenome sequencing and assembly of DNA from four discrete mid-vaginal samples, one of which was obtained from a pregnant woman with trichomoniasis who delivered prematurely.Genes encoding proteins with high similarity to potential virulence factors include two collagenases, a hemolysin, an O-sialoglycoprotein endopeptidase and a feoB-type ferrous iron transport system.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, United States of America; Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia, United States of America.

ABSTRACT
Humans are colonized by thousands of bacterial species, but it is difficult to assess the metabolic and pathogenic potential of the majority of these because they have yet to be cultured. Here, we characterize an uncultivated vaginal mycoplasma tightly associated with trichomoniasis that was previously known by its 16S rRNA sequence as "Mnola." In this study, the mycoplasma was found almost exclusively in women infected with the sexually transmitted pathogen Trichomonas vaginalis, but rarely observed in women with no diagnosed disease. The genomes of four strains of this species were reconstructed using metagenome sequencing and assembly of DNA from four discrete mid-vaginal samples, one of which was obtained from a pregnant woman with trichomoniasis who delivered prematurely. These bacteria harbor several putative virulence factors and display unique metabolic strategies. Genes encoding proteins with high similarity to potential virulence factors include two collagenases, a hemolysin, an O-sialoglycoprotein endopeptidase and a feoB-type ferrous iron transport system. We propose the name "Candidatus Mycoplasma girerdii" for this potential new pathogen.

Show MeSH
Related in: MedlinePlus