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Phasic contractions of the mouse vagina and cervix at different phases of the estrus cycle and during late pregnancy.

Gravina FS, van Helden DF, Kerr KP, de Oliveira RB, Jobling P - PLoS ONE (2014)

Bottom Line: ICs were found in small numbers in the mouse cervix but not in the vagina.Cervical smooth muscle strips taken from mice in estrus, metestrus or late pregnancy were generally spontaneously active.Given that vaginal smooth muscle is normally quiescent then it is likely that increases in hormones such as oxytocin, as might occur through sexual stimulation, enhance the effectiveness of such pacemaking until phasic contractile activity emerges.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Sciences & Pharmacy, Faculty of Health & Medicine, The University of Newcastle, Callaghan, NSW, Australia.

ABSTRACT

Background/aims: The pacemaker mechanisms activating phasic contractions of vaginal and cervical smooth muscle remain poorly understood. Here, we investigate properties of pacemaking in vaginal and cervical tissues by determining whether: 1) functional pacemaking is dependent on the phase of the estrus cycle or pregnancy; 2) pacemaking involves Ca2+ release from sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA) -dependent intracellular Ca2+ stores; and 3) c-Kit and/or vimentin immunoreactive ICs have a role in pacemaking.

Methodology/principal findings: Vaginal and cervical contractions were measured in vitro, as was the distribution of c-Kit and vimentin positive interstitial cells (ICs). Cervical smooth muscle was spontaneously active in estrus and metestrus but quiescent during proestrus and diestrus. Vaginal smooth muscle was normally quiescent but exhibited phasic contractions in the presence of oxytocin or the K+ channel blocker tetraethylammonium (TEA) chloride. Spontaneous contractions in the cervix and TEA-induced phasic contractions in the vagina persisted in the presence of cyclopiazonic acid (CPA), a blocker of the SERCA that refills intracellular SR Ca2+ stores, but were inhibited in low Ca2+ solution or in the presence of nifedipine, an inhibitor of L-type Ca2+channels. ICs were found in small numbers in the mouse cervix but not in the vagina.

Conclusions/significance: Cervical smooth muscle strips taken from mice in estrus, metestrus or late pregnancy were generally spontaneously active. Vaginal smooth muscle strips were normally quiescent but could be induced to exhibit phasic contractions independent on phase of the estrus cycle or late pregnancy. Spontaneous cervical or TEA-induced vaginal phasic contractions were not mediated by ICs or intracellular Ca2+ stores. Given that vaginal smooth muscle is normally quiescent then it is likely that increases in hormones such as oxytocin, as might occur through sexual stimulation, enhance the effectiveness of such pacemaking until phasic contractile activity emerges.

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The effect of the oestrus cycle and late pregnancy on uterine and cervical spontaneous activity.A& C, Frequency of uterine and cervical contractions (per 5 min), indicating that the uterus is spontaneously active throughout the oestrus cycle and the cervix is spontaneously active in estrus, metestrus and in pregnant mice; * mean frequency of cervices from pregnant mice is slightly higher than estrus and metestrus (* P<0.05). This difference is small and has not been further investigated. B & D, the force of uterine and cervical contractions in the different stages of the oestrus cycle and in pregnancy. While significant (* P<0.05), the difference in contraction strength for uterine tisues at proestrus has not been further investigated. Data is presented as the mean ± SE with n = 4–9.
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pone-0111307-g003: The effect of the oestrus cycle and late pregnancy on uterine and cervical spontaneous activity.A& C, Frequency of uterine and cervical contractions (per 5 min), indicating that the uterus is spontaneously active throughout the oestrus cycle and the cervix is spontaneously active in estrus, metestrus and in pregnant mice; * mean frequency of cervices from pregnant mice is slightly higher than estrus and metestrus (* P<0.05). This difference is small and has not been further investigated. B & D, the force of uterine and cervical contractions in the different stages of the oestrus cycle and in pregnancy. While significant (* P<0.05), the difference in contraction strength for uterine tisues at proestrus has not been further investigated. Data is presented as the mean ± SE with n = 4–9.

Mentions: Non-pregnant (A) and late pregnant (D) mice uteri exhibited spontaneous contractions. The cervices from non-pregnant mice (B) exhibited spontaneous contractions in estrus and metestrus, but not diestrus and proestrus (see figure 3 for more details); while cervices from pregnant mice (E) always exhibited spontaneous contractions. Vaginal tissues from non-pregnant (C) and pregnant (F) mice were generally not spontaneously active.


Phasic contractions of the mouse vagina and cervix at different phases of the estrus cycle and during late pregnancy.

Gravina FS, van Helden DF, Kerr KP, de Oliveira RB, Jobling P - PLoS ONE (2014)

The effect of the oestrus cycle and late pregnancy on uterine and cervical spontaneous activity.A& C, Frequency of uterine and cervical contractions (per 5 min), indicating that the uterus is spontaneously active throughout the oestrus cycle and the cervix is spontaneously active in estrus, metestrus and in pregnant mice; * mean frequency of cervices from pregnant mice is slightly higher than estrus and metestrus (* P<0.05). This difference is small and has not been further investigated. B & D, the force of uterine and cervical contractions in the different stages of the oestrus cycle and in pregnancy. While significant (* P<0.05), the difference in contraction strength for uterine tisues at proestrus has not been further investigated. Data is presented as the mean ± SE with n = 4–9.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4206458&req=5

pone-0111307-g003: The effect of the oestrus cycle and late pregnancy on uterine and cervical spontaneous activity.A& C, Frequency of uterine and cervical contractions (per 5 min), indicating that the uterus is spontaneously active throughout the oestrus cycle and the cervix is spontaneously active in estrus, metestrus and in pregnant mice; * mean frequency of cervices from pregnant mice is slightly higher than estrus and metestrus (* P<0.05). This difference is small and has not been further investigated. B & D, the force of uterine and cervical contractions in the different stages of the oestrus cycle and in pregnancy. While significant (* P<0.05), the difference in contraction strength for uterine tisues at proestrus has not been further investigated. Data is presented as the mean ± SE with n = 4–9.
Mentions: Non-pregnant (A) and late pregnant (D) mice uteri exhibited spontaneous contractions. The cervices from non-pregnant mice (B) exhibited spontaneous contractions in estrus and metestrus, but not diestrus and proestrus (see figure 3 for more details); while cervices from pregnant mice (E) always exhibited spontaneous contractions. Vaginal tissues from non-pregnant (C) and pregnant (F) mice were generally not spontaneously active.

Bottom Line: ICs were found in small numbers in the mouse cervix but not in the vagina.Cervical smooth muscle strips taken from mice in estrus, metestrus or late pregnancy were generally spontaneously active.Given that vaginal smooth muscle is normally quiescent then it is likely that increases in hormones such as oxytocin, as might occur through sexual stimulation, enhance the effectiveness of such pacemaking until phasic contractile activity emerges.

View Article: PubMed Central - PubMed

Affiliation: School of Biomedical Sciences & Pharmacy, Faculty of Health & Medicine, The University of Newcastle, Callaghan, NSW, Australia.

ABSTRACT

Background/aims: The pacemaker mechanisms activating phasic contractions of vaginal and cervical smooth muscle remain poorly understood. Here, we investigate properties of pacemaking in vaginal and cervical tissues by determining whether: 1) functional pacemaking is dependent on the phase of the estrus cycle or pregnancy; 2) pacemaking involves Ca2+ release from sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA) -dependent intracellular Ca2+ stores; and 3) c-Kit and/or vimentin immunoreactive ICs have a role in pacemaking.

Methodology/principal findings: Vaginal and cervical contractions were measured in vitro, as was the distribution of c-Kit and vimentin positive interstitial cells (ICs). Cervical smooth muscle was spontaneously active in estrus and metestrus but quiescent during proestrus and diestrus. Vaginal smooth muscle was normally quiescent but exhibited phasic contractions in the presence of oxytocin or the K+ channel blocker tetraethylammonium (TEA) chloride. Spontaneous contractions in the cervix and TEA-induced phasic contractions in the vagina persisted in the presence of cyclopiazonic acid (CPA), a blocker of the SERCA that refills intracellular SR Ca2+ stores, but were inhibited in low Ca2+ solution or in the presence of nifedipine, an inhibitor of L-type Ca2+channels. ICs were found in small numbers in the mouse cervix but not in the vagina.

Conclusions/significance: Cervical smooth muscle strips taken from mice in estrus, metestrus or late pregnancy were generally spontaneously active. Vaginal smooth muscle strips were normally quiescent but could be induced to exhibit phasic contractions independent on phase of the estrus cycle or late pregnancy. Spontaneous cervical or TEA-induced vaginal phasic contractions were not mediated by ICs or intracellular Ca2+ stores. Given that vaginal smooth muscle is normally quiescent then it is likely that increases in hormones such as oxytocin, as might occur through sexual stimulation, enhance the effectiveness of such pacemaking until phasic contractile activity emerges.

Show MeSH
Related in: MedlinePlus