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A panel of overexpressed proteins for prognosis in esophageal squamous cell carcinoma.

Shang L, Liu HJ, Hao JJ, Jiang YY, Shi F, Zhang Y, Cai Y, Xu X, Jia XM, Zhan QM, Wang MR - PLoS ONE (2014)

Bottom Line: Significant associations were found between high expression of PI3K-p85α, EGFR and p53 and poor prognosis (P = 0.00111; 0.00001; 0.00426).Applying these three proteins as an IHC panel could divide patients into different subgroups (P<0.000001).Multivariate cox regression analysis indicated that the three-protein panel was an independent prognostic factor with very high statistical significance (HR = 2.090, 95% CI: 1.621-2.696, P = 0.00000001).

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

ABSTRACT
Esophageal squamous cell carcinoma (ESCC) is a common cancer with poor prognosis. In order to identify useful biomarkers for accurately classifying prognostic risks for ESCC patients, we examined the expression of six proteins by immunohistochemistry (IHC) in 590 paraffin-embedded ESCC samples. The candidate proteins include p53, EGFR, c-KIT, TIMP1 and PI3K-p110α reported to be altered in ESCC tissues as well as another important component of PI3K, PI3K-p85α. Of the six proteins tested, p53, EGFR, c-KIT, TIMP1 and PI3K-p85α were detected with high expression in 43.0%, 36.6%, 55.9%, 70.7% and 57.1% of tumors, respectively. Significant associations were found between high expression of PI3K-p85α, EGFR and p53 and poor prognosis (P = 0.00111; 0.00001; 0.00426). Applying these three proteins as an IHC panel could divide patients into different subgroups (P<0.000001). Multivariate cox regression analysis indicated that the three-protein panel was an independent prognostic factor with very high statistical significance (HR = 2.090, 95% CI: 1.621-2.696, P = 0.00000001). The data suggest that the three-protein panel of PI3K-p85α, EGFR and p53 is an important candidate biomarker for the prognosis of patients with ESCC.

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Related in: MedlinePlus

Representative IHC images of PI3K-p85α, EGFR, p53, c-KIT and TIMP1.IHC results reveal that these proteins are highly expressed in ESCC tumors, whereas a low/no expression in adjacent normal tissues. IHC, immunohistochemistry; ESCC, esophageal squamous cell carcinoma; PI3K, phosphatidylinositol 3-kinases; EGFR, epidermal growth factor receptor; TIMP1, TIMP metallopeptidase inhibitor 1. Original magnification: 200 × and 400 ×.
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pone-0111045-g001: Representative IHC images of PI3K-p85α, EGFR, p53, c-KIT and TIMP1.IHC results reveal that these proteins are highly expressed in ESCC tumors, whereas a low/no expression in adjacent normal tissues. IHC, immunohistochemistry; ESCC, esophageal squamous cell carcinoma; PI3K, phosphatidylinositol 3-kinases; EGFR, epidermal growth factor receptor; TIMP1, TIMP metallopeptidase inhibitor 1. Original magnification: 200 × and 400 ×.

Mentions: High expressions of candidate proteins PI3K-p85α, EGFR, p53, TIMP1 and c-KIT were detected in 57.1% (303/531), 36.6% (185/506), 43.0% (232/539), 70.7% (246/348) and 55.9% (104/186) of tumors, but in normal operative margin, 2.7% (10/368), 3.5% (13/372), 2.9% (11/382), 16.4% (41/250) and 9.6% (13/135), respectively (Figure 1–2). For the expression of PI3K-p110α, however, no significant differences were found between ESCC and adjacent normal epithelial tissues.


A panel of overexpressed proteins for prognosis in esophageal squamous cell carcinoma.

Shang L, Liu HJ, Hao JJ, Jiang YY, Shi F, Zhang Y, Cai Y, Xu X, Jia XM, Zhan QM, Wang MR - PLoS ONE (2014)

Representative IHC images of PI3K-p85α, EGFR, p53, c-KIT and TIMP1.IHC results reveal that these proteins are highly expressed in ESCC tumors, whereas a low/no expression in adjacent normal tissues. IHC, immunohistochemistry; ESCC, esophageal squamous cell carcinoma; PI3K, phosphatidylinositol 3-kinases; EGFR, epidermal growth factor receptor; TIMP1, TIMP metallopeptidase inhibitor 1. Original magnification: 200 × and 400 ×.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4206450&req=5

pone-0111045-g001: Representative IHC images of PI3K-p85α, EGFR, p53, c-KIT and TIMP1.IHC results reveal that these proteins are highly expressed in ESCC tumors, whereas a low/no expression in adjacent normal tissues. IHC, immunohistochemistry; ESCC, esophageal squamous cell carcinoma; PI3K, phosphatidylinositol 3-kinases; EGFR, epidermal growth factor receptor; TIMP1, TIMP metallopeptidase inhibitor 1. Original magnification: 200 × and 400 ×.
Mentions: High expressions of candidate proteins PI3K-p85α, EGFR, p53, TIMP1 and c-KIT were detected in 57.1% (303/531), 36.6% (185/506), 43.0% (232/539), 70.7% (246/348) and 55.9% (104/186) of tumors, but in normal operative margin, 2.7% (10/368), 3.5% (13/372), 2.9% (11/382), 16.4% (41/250) and 9.6% (13/135), respectively (Figure 1–2). For the expression of PI3K-p110α, however, no significant differences were found between ESCC and adjacent normal epithelial tissues.

Bottom Line: Significant associations were found between high expression of PI3K-p85α, EGFR and p53 and poor prognosis (P = 0.00111; 0.00001; 0.00426).Applying these three proteins as an IHC panel could divide patients into different subgroups (P<0.000001).Multivariate cox regression analysis indicated that the three-protein panel was an independent prognostic factor with very high statistical significance (HR = 2.090, 95% CI: 1.621-2.696, P = 0.00000001).

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Molecular Oncology, Cancer Institute (Hospital), Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

ABSTRACT
Esophageal squamous cell carcinoma (ESCC) is a common cancer with poor prognosis. In order to identify useful biomarkers for accurately classifying prognostic risks for ESCC patients, we examined the expression of six proteins by immunohistochemistry (IHC) in 590 paraffin-embedded ESCC samples. The candidate proteins include p53, EGFR, c-KIT, TIMP1 and PI3K-p110α reported to be altered in ESCC tissues as well as another important component of PI3K, PI3K-p85α. Of the six proteins tested, p53, EGFR, c-KIT, TIMP1 and PI3K-p85α were detected with high expression in 43.0%, 36.6%, 55.9%, 70.7% and 57.1% of tumors, respectively. Significant associations were found between high expression of PI3K-p85α, EGFR and p53 and poor prognosis (P = 0.00111; 0.00001; 0.00426). Applying these three proteins as an IHC panel could divide patients into different subgroups (P<0.000001). Multivariate cox regression analysis indicated that the three-protein panel was an independent prognostic factor with very high statistical significance (HR = 2.090, 95% CI: 1.621-2.696, P = 0.00000001). The data suggest that the three-protein panel of PI3K-p85α, EGFR and p53 is an important candidate biomarker for the prognosis of patients with ESCC.

Show MeSH
Related in: MedlinePlus