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Effects of endophyte colonization of Vicia faba (Fabaceae) plants on the life-history of leafminer parasitoids Phaedrotoma scabriventris (hymenoptera: braconidae) and Diglyphus isaea (hymenoptera: eulophidae).

Akutse KS, Fiaboe KK, Van den Berg J, Ekesi S, Maniania NK - PLoS ONE (2014)

Bottom Line: No significant difference was observed between the control and the endophyte-inoculated plants in terms of parasitism rates of P. scabriventris (p = 0.68) and D. isaea (p = 0.45) and adult' survival times (p = 0.06).The survival period of the F1 progeny of P. scabriventris was reduced (p<0.0001) in B. bassiana S4SU1 to 28 days as compared to more than 40 days for B. bassiana G1LU3, ICIPE 279 and H. lixii F3ST1.However, no significant difference (p = 0.54) was observed in the survival times of the F1 progeny of D. isaea.

View Article: PubMed Central - PubMed

Affiliation: Plant Health Division, International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya; Unit of Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.

ABSTRACT
Effects of the fungal endophytes Beauveria bassiana (isolates ICIPE 279, G1LU3, S4SU1) and Hypocrea lixii (isolate F3ST1) on the life-history of Phaedrotoma scabriventris and Diglyphus isaea, parasitoids of the pea leafminer Liriomyza huidobrensis, were studied in the laboratory. Parasitoids were allowed to parasitize 2(nd) and 3(rd) instar L. huidobrensis larvae reared on endophytically-inoculated faba bean, Vicia faba. In the control, parasitoids were reared on non-inoculated host plants. Parasitism, pupation, adult emergence and survival were recorded. No significant difference was observed between the control and the endophyte-inoculated plants in terms of parasitism rates of P. scabriventris (p = 0.68) and D. isaea (p = 0.45) and adult' survival times (p = 0.06). The survival period of the F1 progeny of P. scabriventris was reduced (p<0.0001) in B. bassiana S4SU1 to 28 days as compared to more than 40 days for B. bassiana G1LU3, ICIPE 279 and H. lixii F3ST1. However, no significant difference (p = 0.54) was observed in the survival times of the F1 progeny of D. isaea. This study has demonstrated that together, endophytes and parasitoids have beneficial effects in L. huidobrensis population suppression.

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Progeny survival curves of Phaedrotoma scabriventris emerging from Vicia faba plants endophytically-colonized by different fungal isolates of Beauveria bassiana (S4SU1, G1LU3 and ICIPE279) and Hypocrea lixii (F3ST1) and infested with 2nd and 3rd instar larvae of Liriomyza huidobrensis.
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pone-0109965-g008: Progeny survival curves of Phaedrotoma scabriventris emerging from Vicia faba plants endophytically-colonized by different fungal isolates of Beauveria bassiana (S4SU1, G1LU3 and ICIPE279) and Hypocrea lixii (F3ST1) and infested with 2nd and 3rd instar larvae of Liriomyza huidobrensis.

Mentions: The median survival times of the F1 P. scabriventris progenies were not significantly different among the treatments (F = 1.42, df = 4, 9, p = 0.304) (Table 3). However, the survival curves of the progeny differed significantly among the treatments (proximate log rank test = 56.473, df = 4, p<0.0001) (Fig. 8). At 7 days post-emergence, survival was approximately 90% in all the treatments, except in B. bassiana S4SU1 where it was 76.7%. At day 28, no survival (0%) was observed in B. bassiana S4SU1 while it was 60, 52.5, 33.3 and 26.7% in H. lixii F3ST1, control, B. bassiana ICIPE 279 and G1LU3, respectively (Fig. 8). At day 40, the lowest survival of progeny was recorded in B. bassiana ICIPE G1LU3, followed by B. bassiana ICIPE 279 (Fig. 8).


Effects of endophyte colonization of Vicia faba (Fabaceae) plants on the life-history of leafminer parasitoids Phaedrotoma scabriventris (hymenoptera: braconidae) and Diglyphus isaea (hymenoptera: eulophidae).

Akutse KS, Fiaboe KK, Van den Berg J, Ekesi S, Maniania NK - PLoS ONE (2014)

Progeny survival curves of Phaedrotoma scabriventris emerging from Vicia faba plants endophytically-colonized by different fungal isolates of Beauveria bassiana (S4SU1, G1LU3 and ICIPE279) and Hypocrea lixii (F3ST1) and infested with 2nd and 3rd instar larvae of Liriomyza huidobrensis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4206285&req=5

pone-0109965-g008: Progeny survival curves of Phaedrotoma scabriventris emerging from Vicia faba plants endophytically-colonized by different fungal isolates of Beauveria bassiana (S4SU1, G1LU3 and ICIPE279) and Hypocrea lixii (F3ST1) and infested with 2nd and 3rd instar larvae of Liriomyza huidobrensis.
Mentions: The median survival times of the F1 P. scabriventris progenies were not significantly different among the treatments (F = 1.42, df = 4, 9, p = 0.304) (Table 3). However, the survival curves of the progeny differed significantly among the treatments (proximate log rank test = 56.473, df = 4, p<0.0001) (Fig. 8). At 7 days post-emergence, survival was approximately 90% in all the treatments, except in B. bassiana S4SU1 where it was 76.7%. At day 28, no survival (0%) was observed in B. bassiana S4SU1 while it was 60, 52.5, 33.3 and 26.7% in H. lixii F3ST1, control, B. bassiana ICIPE 279 and G1LU3, respectively (Fig. 8). At day 40, the lowest survival of progeny was recorded in B. bassiana ICIPE G1LU3, followed by B. bassiana ICIPE 279 (Fig. 8).

Bottom Line: No significant difference was observed between the control and the endophyte-inoculated plants in terms of parasitism rates of P. scabriventris (p = 0.68) and D. isaea (p = 0.45) and adult' survival times (p = 0.06).The survival period of the F1 progeny of P. scabriventris was reduced (p<0.0001) in B. bassiana S4SU1 to 28 days as compared to more than 40 days for B. bassiana G1LU3, ICIPE 279 and H. lixii F3ST1.However, no significant difference (p = 0.54) was observed in the survival times of the F1 progeny of D. isaea.

View Article: PubMed Central - PubMed

Affiliation: Plant Health Division, International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya; Unit of Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.

ABSTRACT
Effects of the fungal endophytes Beauveria bassiana (isolates ICIPE 279, G1LU3, S4SU1) and Hypocrea lixii (isolate F3ST1) on the life-history of Phaedrotoma scabriventris and Diglyphus isaea, parasitoids of the pea leafminer Liriomyza huidobrensis, were studied in the laboratory. Parasitoids were allowed to parasitize 2(nd) and 3(rd) instar L. huidobrensis larvae reared on endophytically-inoculated faba bean, Vicia faba. In the control, parasitoids were reared on non-inoculated host plants. Parasitism, pupation, adult emergence and survival were recorded. No significant difference was observed between the control and the endophyte-inoculated plants in terms of parasitism rates of P. scabriventris (p = 0.68) and D. isaea (p = 0.45) and adult' survival times (p = 0.06). The survival period of the F1 progeny of P. scabriventris was reduced (p<0.0001) in B. bassiana S4SU1 to 28 days as compared to more than 40 days for B. bassiana G1LU3, ICIPE 279 and H. lixii F3ST1. However, no significant difference (p = 0.54) was observed in the survival times of the F1 progeny of D. isaea. This study has demonstrated that together, endophytes and parasitoids have beneficial effects in L. huidobrensis population suppression.

Show MeSH
Related in: MedlinePlus