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Effects of endophyte colonization of Vicia faba (Fabaceae) plants on the life-history of leafminer parasitoids Phaedrotoma scabriventris (hymenoptera: braconidae) and Diglyphus isaea (hymenoptera: eulophidae).

Akutse KS, Fiaboe KK, Van den Berg J, Ekesi S, Maniania NK - PLoS ONE (2014)

Bottom Line: No significant difference was observed between the control and the endophyte-inoculated plants in terms of parasitism rates of P. scabriventris (p = 0.68) and D. isaea (p = 0.45) and adult' survival times (p = 0.06).The survival period of the F1 progeny of P. scabriventris was reduced (p<0.0001) in B. bassiana S4SU1 to 28 days as compared to more than 40 days for B. bassiana G1LU3, ICIPE 279 and H. lixii F3ST1.However, no significant difference (p = 0.54) was observed in the survival times of the F1 progeny of D. isaea.

View Article: PubMed Central - PubMed

Affiliation: Plant Health Division, International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya; Unit of Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.

ABSTRACT
Effects of the fungal endophytes Beauveria bassiana (isolates ICIPE 279, G1LU3, S4SU1) and Hypocrea lixii (isolate F3ST1) on the life-history of Phaedrotoma scabriventris and Diglyphus isaea, parasitoids of the pea leafminer Liriomyza huidobrensis, were studied in the laboratory. Parasitoids were allowed to parasitize 2(nd) and 3(rd) instar L. huidobrensis larvae reared on endophytically-inoculated faba bean, Vicia faba. In the control, parasitoids were reared on non-inoculated host plants. Parasitism, pupation, adult emergence and survival were recorded. No significant difference was observed between the control and the endophyte-inoculated plants in terms of parasitism rates of P. scabriventris (p = 0.68) and D. isaea (p = 0.45) and adult' survival times (p = 0.06). The survival period of the F1 progeny of P. scabriventris was reduced (p<0.0001) in B. bassiana S4SU1 to 28 days as compared to more than 40 days for B. bassiana G1LU3, ICIPE 279 and H. lixii F3ST1. However, no significant difference (p = 0.54) was observed in the survival times of the F1 progeny of D. isaea. This study has demonstrated that together, endophytes and parasitoids have beneficial effects in L. huidobrensis population suppression.

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Survival curves for Diglyphus isaea adults parasitizing 2nd and 3rd instar Liriomyza huidobrensis larvae following exposure to Vicia faba plants endophytically-colonized by different fungal isolates of Beauveria bassiana (S4SU1, G1LU3 and ICIPE 279) and Hypocrea lixii F3ST1 after 40 days post-exposure.
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pone-0109965-g001: Survival curves for Diglyphus isaea adults parasitizing 2nd and 3rd instar Liriomyza huidobrensis larvae following exposure to Vicia faba plants endophytically-colonized by different fungal isolates of Beauveria bassiana (S4SU1, G1LU3 and ICIPE 279) and Hypocrea lixii F3ST1 after 40 days post-exposure.

Mentions: Median survival time of D. isaea adults was 29.3 days in the control and varied between 21.2 and 24.5 days in fungal endophyte treatments, which were not significantly different (Table 2). The survival time curves showed no significant differences between the treatments including the control up to 14 days (F = 2.3, df = 4, 555, p = 0.056) but differed significantly among the treatments thereafter (proximate log rank test = 19.48, df = 4, p<0.0001) (Fig. 1). For example, at 29 days post-exposure, 52% D. isaea survived in the control while 43.3, 33.3, 38.7 and 39.3% in B. bassiana G1LU3, ICIPE 279, S4SU1 and H. lixii, respectively (Fig. 1). At 40 days post-exposure, the survival was 36.0% in the control and reduced to 10.7, 13.3, 24.7 and 19.3% in the B. bassiana ICIPE 279, S4SU1, G1LU3 and H. lixii F3ST1 treatments, respectively (Fig. 1).


Effects of endophyte colonization of Vicia faba (Fabaceae) plants on the life-history of leafminer parasitoids Phaedrotoma scabriventris (hymenoptera: braconidae) and Diglyphus isaea (hymenoptera: eulophidae).

Akutse KS, Fiaboe KK, Van den Berg J, Ekesi S, Maniania NK - PLoS ONE (2014)

Survival curves for Diglyphus isaea adults parasitizing 2nd and 3rd instar Liriomyza huidobrensis larvae following exposure to Vicia faba plants endophytically-colonized by different fungal isolates of Beauveria bassiana (S4SU1, G1LU3 and ICIPE 279) and Hypocrea lixii F3ST1 after 40 days post-exposure.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4206285&req=5

pone-0109965-g001: Survival curves for Diglyphus isaea adults parasitizing 2nd and 3rd instar Liriomyza huidobrensis larvae following exposure to Vicia faba plants endophytically-colonized by different fungal isolates of Beauveria bassiana (S4SU1, G1LU3 and ICIPE 279) and Hypocrea lixii F3ST1 after 40 days post-exposure.
Mentions: Median survival time of D. isaea adults was 29.3 days in the control and varied between 21.2 and 24.5 days in fungal endophyte treatments, which were not significantly different (Table 2). The survival time curves showed no significant differences between the treatments including the control up to 14 days (F = 2.3, df = 4, 555, p = 0.056) but differed significantly among the treatments thereafter (proximate log rank test = 19.48, df = 4, p<0.0001) (Fig. 1). For example, at 29 days post-exposure, 52% D. isaea survived in the control while 43.3, 33.3, 38.7 and 39.3% in B. bassiana G1LU3, ICIPE 279, S4SU1 and H. lixii, respectively (Fig. 1). At 40 days post-exposure, the survival was 36.0% in the control and reduced to 10.7, 13.3, 24.7 and 19.3% in the B. bassiana ICIPE 279, S4SU1, G1LU3 and H. lixii F3ST1 treatments, respectively (Fig. 1).

Bottom Line: No significant difference was observed between the control and the endophyte-inoculated plants in terms of parasitism rates of P. scabriventris (p = 0.68) and D. isaea (p = 0.45) and adult' survival times (p = 0.06).The survival period of the F1 progeny of P. scabriventris was reduced (p<0.0001) in B. bassiana S4SU1 to 28 days as compared to more than 40 days for B. bassiana G1LU3, ICIPE 279 and H. lixii F3ST1.However, no significant difference (p = 0.54) was observed in the survival times of the F1 progeny of D. isaea.

View Article: PubMed Central - PubMed

Affiliation: Plant Health Division, International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya; Unit of Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.

ABSTRACT
Effects of the fungal endophytes Beauveria bassiana (isolates ICIPE 279, G1LU3, S4SU1) and Hypocrea lixii (isolate F3ST1) on the life-history of Phaedrotoma scabriventris and Diglyphus isaea, parasitoids of the pea leafminer Liriomyza huidobrensis, were studied in the laboratory. Parasitoids were allowed to parasitize 2(nd) and 3(rd) instar L. huidobrensis larvae reared on endophytically-inoculated faba bean, Vicia faba. In the control, parasitoids were reared on non-inoculated host plants. Parasitism, pupation, adult emergence and survival were recorded. No significant difference was observed between the control and the endophyte-inoculated plants in terms of parasitism rates of P. scabriventris (p = 0.68) and D. isaea (p = 0.45) and adult' survival times (p = 0.06). The survival period of the F1 progeny of P. scabriventris was reduced (p<0.0001) in B. bassiana S4SU1 to 28 days as compared to more than 40 days for B. bassiana G1LU3, ICIPE 279 and H. lixii F3ST1. However, no significant difference (p = 0.54) was observed in the survival times of the F1 progeny of D. isaea. This study has demonstrated that together, endophytes and parasitoids have beneficial effects in L. huidobrensis population suppression.

Show MeSH
Related in: MedlinePlus