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The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing.

Mohan AM, Bibby KJ, Lipus D, Hammack RW, Gregory KB - PLoS ONE (2014)

Bottom Line: Here, for the first time, we present results from a shotgun metagenome of microbial communities in both hydraulic fracturing source water and wastewater produced by hydraulic fracturing.Taxonomic analyses showed an increase in anaerobic/facultative anaerobic classes related to Clostridia, Gammaproteobacteria, Bacteroidia and Epsilonproteobacteria in produced water as compared to predominantly aerobic Alphaproteobacteria in the fracturing source water.These results suggest that microbial communities in produced water have an increased genetic ability to handle stress, which has significant implications for produced water management, such as disinfection.

View Article: PubMed Central - PubMed

Affiliation: National Energy Technology Laboratory, Pittsburgh, Pennsylvania, United States of America; Department of Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America.

ABSTRACT
Microbial activity in produced water from hydraulic fracturing operations can lead to undesired environmental impacts and increase gas production costs. However, the metabolic profile of these microbial communities is not well understood. Here, for the first time, we present results from a shotgun metagenome of microbial communities in both hydraulic fracturing source water and wastewater produced by hydraulic fracturing. Taxonomic analyses showed an increase in anaerobic/facultative anaerobic classes related to Clostridia, Gammaproteobacteria, Bacteroidia and Epsilonproteobacteria in produced water as compared to predominantly aerobic Alphaproteobacteria in the fracturing source water. The metabolic profile revealed a relative increase in genes responsible for carbohydrate metabolism, respiration, sporulation and dormancy, iron acquisition and metabolism, stress response and sulfur metabolism in the produced water samples. These results suggest that microbial communities in produced water have an increased genetic ability to handle stress, which has significant implications for produced water management, such as disinfection.

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Order level affiliations assigned to contigs with predicted proteins and rRNA genes in source water (SW), produced water day 1 (PW day 1) and produced water day 9 (PW day 9).Total community includes Bacteria, Archaea, Viruses and Eukaryota. Only orders representing >2% of the total community are shown in the figure.
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pone-0107682-g002: Order level affiliations assigned to contigs with predicted proteins and rRNA genes in source water (SW), produced water day 1 (PW day 1) and produced water day 9 (PW day 9).Total community includes Bacteria, Archaea, Viruses and Eukaryota. Only orders representing >2% of the total community are shown in the figure.

Mentions: Bacteria constituted the dominant domain (97–99% of the total community) in all samples. However, a shift in bacterial community composition was detected between the samples at the class and order levels (Figure 1, 2). Contigs affiliated to the class Alphaproteobacteria constituted the majority of the community in the fracturing source water (81%) and produced water day 1 (67%) samples (Figure 1). Within Alphaproteobacteria, the dominant order detected was Rhodobacterales (68–88% of the Alphaproteobacteria; 55–59% of the total community) in both the source water and produced water day 1 samples (Figure 2). The relative abundance of Alphaproteobacteria decreased to <2% of the community in the produced water day 9 sample. Previous qPCR analysis of these samples suggests that that the total bacterial population remained constant at 106–107 copies of 16S RNA gene/ml [5].


The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing.

Mohan AM, Bibby KJ, Lipus D, Hammack RW, Gregory KB - PLoS ONE (2014)

Order level affiliations assigned to contigs with predicted proteins and rRNA genes in source water (SW), produced water day 1 (PW day 1) and produced water day 9 (PW day 9).Total community includes Bacteria, Archaea, Viruses and Eukaryota. Only orders representing >2% of the total community are shown in the figure.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4206270&req=5

pone-0107682-g002: Order level affiliations assigned to contigs with predicted proteins and rRNA genes in source water (SW), produced water day 1 (PW day 1) and produced water day 9 (PW day 9).Total community includes Bacteria, Archaea, Viruses and Eukaryota. Only orders representing >2% of the total community are shown in the figure.
Mentions: Bacteria constituted the dominant domain (97–99% of the total community) in all samples. However, a shift in bacterial community composition was detected between the samples at the class and order levels (Figure 1, 2). Contigs affiliated to the class Alphaproteobacteria constituted the majority of the community in the fracturing source water (81%) and produced water day 1 (67%) samples (Figure 1). Within Alphaproteobacteria, the dominant order detected was Rhodobacterales (68–88% of the Alphaproteobacteria; 55–59% of the total community) in both the source water and produced water day 1 samples (Figure 2). The relative abundance of Alphaproteobacteria decreased to <2% of the community in the produced water day 9 sample. Previous qPCR analysis of these samples suggests that that the total bacterial population remained constant at 106–107 copies of 16S RNA gene/ml [5].

Bottom Line: Here, for the first time, we present results from a shotgun metagenome of microbial communities in both hydraulic fracturing source water and wastewater produced by hydraulic fracturing.Taxonomic analyses showed an increase in anaerobic/facultative anaerobic classes related to Clostridia, Gammaproteobacteria, Bacteroidia and Epsilonproteobacteria in produced water as compared to predominantly aerobic Alphaproteobacteria in the fracturing source water.These results suggest that microbial communities in produced water have an increased genetic ability to handle stress, which has significant implications for produced water management, such as disinfection.

View Article: PubMed Central - PubMed

Affiliation: National Energy Technology Laboratory, Pittsburgh, Pennsylvania, United States of America; Department of Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America.

ABSTRACT
Microbial activity in produced water from hydraulic fracturing operations can lead to undesired environmental impacts and increase gas production costs. However, the metabolic profile of these microbial communities is not well understood. Here, for the first time, we present results from a shotgun metagenome of microbial communities in both hydraulic fracturing source water and wastewater produced by hydraulic fracturing. Taxonomic analyses showed an increase in anaerobic/facultative anaerobic classes related to Clostridia, Gammaproteobacteria, Bacteroidia and Epsilonproteobacteria in produced water as compared to predominantly aerobic Alphaproteobacteria in the fracturing source water. The metabolic profile revealed a relative increase in genes responsible for carbohydrate metabolism, respiration, sporulation and dormancy, iron acquisition and metabolism, stress response and sulfur metabolism in the produced water samples. These results suggest that microbial communities in produced water have an increased genetic ability to handle stress, which has significant implications for produced water management, such as disinfection.

Show MeSH
Related in: MedlinePlus