Development of microsatellite markers and detection of genetic variation between Goniozus wasp populations.
Bottom Line: Neither G. nephantidis nor G. legneri were polymorphic within populations.However, there were six primer pairs that did show polymorphism between G. legneri populations that originated from different geographical areas within South America (Uruguay and Chile).One of the markers was subsequently used to provide unbiased assessment of primary sex ratio in G. legneri.
Affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12, 5RD, UK firstname.lastname@example.org.Show MeSH
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Mentions: The results of the capillary fragment analysis were processed using the CEQ 8000 DNA sequencer to determine amplified product size. Neither G. nephantidis nor G. legneri were polymorphic within strains. Nonetheless, there were six primers that showed clear inter-strain polymorphism in G. legneri (Table 3). For instance, primer GlSSR7 showed a large size difference between strains, while U-strain was 137 bp and both Chilean populations were 153 bp. Furthermore, primer GlSSR5 showed a different size allele at 228 bp for both U and C-lab strains, with 224 bp for the C-field strain (Figure 3). Dendrogram analysis based on the overall microsatellite alleles patterns showed clear differences between populations from the two different geographical locations, Uruguay and Chile, as well as differentiation within the two strains collected in Chile (Clab and C-field). However, both strains were more closely related to each other than either was to the Uruguay strain (Figure 4).
Affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12, 5RD, UK email@example.com.