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Development of microsatellite markers and detection of genetic variation between Goniozus wasp populations.

Khidr SK, Hardy IC, Zaviezo T, Mayes S - J. Insect Sci. (2014)

Bottom Line: Neither G. nephantidis nor G. legneri were polymorphic within populations.However, there were six primer pairs that did show polymorphism between G. legneri populations that originated from different geographical areas within South America (Uruguay and Chile).One of the markers was subsequently used to provide unbiased assessment of primary sex ratio in G. legneri.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12, 5RD, UK sahand_kkh@yahoo.com.

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Gel plage of PCR products for primers 7 and 8 of Goniozus legneri. High quality figures are available online.
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f02_01: Gel plage of PCR products for primers 7 and 8 of Goniozus legneri. High quality figures are available online.

Mentions: Primers chosen in the annealing test were amplified on the ABI PCR machine at different temperatures according to their annealing test optima. The gel electrophoresis results were visualized using UV light. Some primers were rejected before capillary testing due to lack of amplification in an annealing test or, if they amplified, not displaying clear/discrete single bands on the gel. PCR products of primers 7 and 8 amplified from G. legneri are shown in Figure 2.


Development of microsatellite markers and detection of genetic variation between Goniozus wasp populations.

Khidr SK, Hardy IC, Zaviezo T, Mayes S - J. Insect Sci. (2014)

Gel plage of PCR products for primers 7 and 8 of Goniozus legneri. High quality figures are available online.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4206244&req=5

f02_01: Gel plage of PCR products for primers 7 and 8 of Goniozus legneri. High quality figures are available online.
Mentions: Primers chosen in the annealing test were amplified on the ABI PCR machine at different temperatures according to their annealing test optima. The gel electrophoresis results were visualized using UV light. Some primers were rejected before capillary testing due to lack of amplification in an annealing test or, if they amplified, not displaying clear/discrete single bands on the gel. PCR products of primers 7 and 8 amplified from G. legneri are shown in Figure 2.

Bottom Line: Neither G. nephantidis nor G. legneri were polymorphic within populations.However, there were six primer pairs that did show polymorphism between G. legneri populations that originated from different geographical areas within South America (Uruguay and Chile).One of the markers was subsequently used to provide unbiased assessment of primary sex ratio in G. legneri.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12, 5RD, UK sahand_kkh@yahoo.com.

Show MeSH
Related in: MedlinePlus