Development of microsatellite markers and detection of genetic variation between Goniozus wasp populations.
Bottom Line: Neither G. nephantidis nor G. legneri were polymorphic within populations.However, there were six primer pairs that did show polymorphism between G. legneri populations that originated from different geographical areas within South America (Uruguay and Chile).One of the markers was subsequently used to provide unbiased assessment of primary sex ratio in G. legneri.
Affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12, 5RD, UK firstname.lastname@example.org.Show MeSH
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Mentions: PCR analysis was performed to optimize annealing gradients for the 12 new G. nephantidis primer pairs and 24 primer pairs for G. legneri strains. Occasionally a number of primer pairs were designed to the same microsatellite repeat sequence to increase the probability of successful amplification. The process was repeated several times to test the reliability of the new primers. Representative results of the annealing electrophoresis gels for G. legneri primers are shown in Figure 1, with the left hand column showing the 2-log DNA ladder (New England Biolabs).
Affiliation: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12, 5RD, UK email@example.com.