Identification and analysis of the pigment composition and sources in the colored cocoon of the silkworm, Bombyx mori, by HPLC-DAD.
Bottom Line: It was found that the mulberry leaf mainly contained four types of pigment: lutein (30.86%), β-carotene (26.3%), chlorophyll a (24.62%), and chlorophyll b (18.21%).The results showed that there were generally two kinds of carotenoids (lutein and β-carotene) in the silk gland and cocoon shell, a little violaxanthin was detected in silk gland, and the pigment found in the blood was mainly lutein in all varieties of silkworm tested.The method used to detect visible pigments reported here could be used to breed new colors of cocoons and to develop and utilize the pigments found in mulberry.
Affiliation: Silk Biotechnology Laboratory, School of Basic Medical and Biological Sciences, Soochow University, No. 199, 702-2303 Room, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China email@example.com.Show MeSH
Mentions: Five varieties of yellow-red cocoon shells and their corresponding blood and silk glands and cartenoid concentrations (Figure 5) were measured. Figure 6 shows chromatograms for the three tissues and histograms, which show variations in carotenoid concentrations between the tissues. Only lutein and β-carotene were detected in the cocoon shells and blood, but in the silk gland, in addition to the above two pigments, a small amount of a third pigment, violaxanthin, was also detected. This may be because violaxanthin was the product produced when lutein and β-carotene were modified in the silk glands. This needs further investigating.
Affiliation: Silk Biotechnology Laboratory, School of Basic Medical and Biological Sciences, Soochow University, No. 199, 702-2303 Room, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China firstname.lastname@example.org.