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Identification and analysis of the pigment composition and sources in the colored cocoon of the silkworm, Bombyx mori, by HPLC-DAD.

Zhu L, Zhang YQ - J. Insect Sci. (2014)

Bottom Line: It was found that the mulberry leaf mainly contained four types of pigment: lutein (30.86%), β-carotene (26.3%), chlorophyll a (24.62%), and chlorophyll b (18.21%).The results showed that there were generally two kinds of carotenoids (lutein and β-carotene) in the silk gland and cocoon shell, a little violaxanthin was detected in silk gland, and the pigment found in the blood was mainly lutein in all varieties of silkworm tested.The method used to detect visible pigments reported here could be used to breed new colors of cocoons and to develop and utilize the pigments found in mulberry.

View Article: PubMed Central - PubMed

Affiliation: Silk Biotechnology Laboratory, School of Basic Medical and Biological Sciences, Soochow University, No. 199, 702-2303 Room, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China sericult@suda.edu.cn.

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Standard curves of five standard samples, a: lutein, b: β-carotene, cviolaxanthin , d: chlorophyll a, e: chlorophyll b. The data in the figure are average values (±SD) of three repeated measurements. High quality figures are available online.
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f02_00: Standard curves of five standard samples, a: lutein, b: β-carotene, cviolaxanthin , d: chlorophyll a, e: chlorophyll b. The data in the figure are average values (±SD) of three repeated measurements. High quality figures are available online.

Mentions: Figure 1 shows the chromatogram for the standard samples and their UV-visible spectra (Figure 1). Taking the concentration of a standard sample as Y and the chromatographic peak area as X, it is possible to draw a standard curve (Figure 2).


Identification and analysis of the pigment composition and sources in the colored cocoon of the silkworm, Bombyx mori, by HPLC-DAD.

Zhu L, Zhang YQ - J. Insect Sci. (2014)

Standard curves of five standard samples, a: lutein, b: β-carotene, cviolaxanthin , d: chlorophyll a, e: chlorophyll b. The data in the figure are average values (±SD) of three repeated measurements. High quality figures are available online.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4206223&req=5

f02_00: Standard curves of five standard samples, a: lutein, b: β-carotene, cviolaxanthin , d: chlorophyll a, e: chlorophyll b. The data in the figure are average values (±SD) of three repeated measurements. High quality figures are available online.
Mentions: Figure 1 shows the chromatogram for the standard samples and their UV-visible spectra (Figure 1). Taking the concentration of a standard sample as Y and the chromatographic peak area as X, it is possible to draw a standard curve (Figure 2).

Bottom Line: It was found that the mulberry leaf mainly contained four types of pigment: lutein (30.86%), β-carotene (26.3%), chlorophyll a (24.62%), and chlorophyll b (18.21%).The results showed that there were generally two kinds of carotenoids (lutein and β-carotene) in the silk gland and cocoon shell, a little violaxanthin was detected in silk gland, and the pigment found in the blood was mainly lutein in all varieties of silkworm tested.The method used to detect visible pigments reported here could be used to breed new colors of cocoons and to develop and utilize the pigments found in mulberry.

View Article: PubMed Central - PubMed

Affiliation: Silk Biotechnology Laboratory, School of Basic Medical and Biological Sciences, Soochow University, No. 199, 702-2303 Room, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China sericult@suda.edu.cn.

Show MeSH