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Influence of caffeine and hyaluronic acid on collagen biosynthesis in human skin fibroblasts.

Donejko M, Przylipiak A, Rysiak E, Głuszuk K, Surażyński A - Drug Des Devel Ther (2014)

Bottom Line: HA, however, did not have any significant effect on this process.Caffeine reduces collagen synthesis in human cultured skin fibroblasts.HA did not have any significant protective effect on this process.

View Article: PubMed Central - PubMed

Affiliation: Department of Esthetic Medicine, Faculty of Pharmacy, Medical University of Białystok, Białystok, Poland.

ABSTRACT

Aim: The aim of this study was to evaluate the effect of caffeine on collagen biosynthesis in human skin fibroblasts and the influence of hyaluronic acid (HA) on this process.

Materials and methods: Collagen, [(3)H]-thymidine incorporation, and prolidase activity were measured in confluent human skin fibroblast cultures that had been treated with 1, 2, and 5 mM caffeine and with caffeine and 500 μg/mL HA. Western immunoblot analysis was performed to evaluate expression of β1-integrin receptor, insulin-like growth factor receptor phospho-Akt protein and mitogen-activated protein kinase (phospho-extracellular signal-regulated kinase).

Results: Caffeine inhibited collagen biosynthesis in a dose-dependent manner. The mechanism of this process was found at the level of prolidase activity. Caffeine significantly inhibited the enzyme activity. The addition of HA had no effect on collagen biosynthesis or prolidase activity in fibroblasts incubated with caffeine. Caffeine also had an inhibitory effect on DNA biosynthesis. HA, however, did not have any significant effect on this process. The inhibition of the expression of β1-integrin and insulin-like growth factor receptor in fibroblasts incubated with the caffeine indicates a possible mechanism of inhibition of collagen biosynthesis.

Conclusion: Caffeine reduces collagen synthesis in human cultured skin fibroblasts. HA did not have any significant protective effect on this process. This is the first study to our knowledge that reports caffeine-induced inhibition of collagen synthesis in human skin fibroblasts.

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DNA biosynthesis measured as [3H]-thymidine incorporation into DNA in human skin fibroblasts (control) incubated for 24 hours with different concentrations of caffeine and hyaluronic acid (HA).Note: Error bars represent ± standard deviation; n=3.
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f3-dddt-8-1923: DNA biosynthesis measured as [3H]-thymidine incorporation into DNA in human skin fibroblasts (control) incubated for 24 hours with different concentrations of caffeine and hyaluronic acid (HA).Note: Error bars represent ± standard deviation; n=3.

Mentions: Research conducted so far states that caffeine has an inhibitory effect on cell division. For this reason, the influence of caffeine on DNA biosynthesis was also assessed. The dpm values of [3H]-thymidine incorporated in the DNA of these cells were assumed to constitute 100% (Figure 3). It has been ascertained that caffeine in a dose-dependent manner inhibits the biosynthesis of DNA. At the highest concentration, DNA biosynthesis was inhibited by 32%±0.98%. HA did not have any significant protective effect in counteracting the inhibition of DNA biosynthesis caused by the increased concentrations of caffeine.


Influence of caffeine and hyaluronic acid on collagen biosynthesis in human skin fibroblasts.

Donejko M, Przylipiak A, Rysiak E, Głuszuk K, Surażyński A - Drug Des Devel Ther (2014)

DNA biosynthesis measured as [3H]-thymidine incorporation into DNA in human skin fibroblasts (control) incubated for 24 hours with different concentrations of caffeine and hyaluronic acid (HA).Note: Error bars represent ± standard deviation; n=3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4206198&req=5

f3-dddt-8-1923: DNA biosynthesis measured as [3H]-thymidine incorporation into DNA in human skin fibroblasts (control) incubated for 24 hours with different concentrations of caffeine and hyaluronic acid (HA).Note: Error bars represent ± standard deviation; n=3.
Mentions: Research conducted so far states that caffeine has an inhibitory effect on cell division. For this reason, the influence of caffeine on DNA biosynthesis was also assessed. The dpm values of [3H]-thymidine incorporated in the DNA of these cells were assumed to constitute 100% (Figure 3). It has been ascertained that caffeine in a dose-dependent manner inhibits the biosynthesis of DNA. At the highest concentration, DNA biosynthesis was inhibited by 32%±0.98%. HA did not have any significant protective effect in counteracting the inhibition of DNA biosynthesis caused by the increased concentrations of caffeine.

Bottom Line: HA, however, did not have any significant effect on this process.Caffeine reduces collagen synthesis in human cultured skin fibroblasts.HA did not have any significant protective effect on this process.

View Article: PubMed Central - PubMed

Affiliation: Department of Esthetic Medicine, Faculty of Pharmacy, Medical University of Białystok, Białystok, Poland.

ABSTRACT

Aim: The aim of this study was to evaluate the effect of caffeine on collagen biosynthesis in human skin fibroblasts and the influence of hyaluronic acid (HA) on this process.

Materials and methods: Collagen, [(3)H]-thymidine incorporation, and prolidase activity were measured in confluent human skin fibroblast cultures that had been treated with 1, 2, and 5 mM caffeine and with caffeine and 500 μg/mL HA. Western immunoblot analysis was performed to evaluate expression of β1-integrin receptor, insulin-like growth factor receptor phospho-Akt protein and mitogen-activated protein kinase (phospho-extracellular signal-regulated kinase).

Results: Caffeine inhibited collagen biosynthesis in a dose-dependent manner. The mechanism of this process was found at the level of prolidase activity. Caffeine significantly inhibited the enzyme activity. The addition of HA had no effect on collagen biosynthesis or prolidase activity in fibroblasts incubated with caffeine. Caffeine also had an inhibitory effect on DNA biosynthesis. HA, however, did not have any significant effect on this process. The inhibition of the expression of β1-integrin and insulin-like growth factor receptor in fibroblasts incubated with the caffeine indicates a possible mechanism of inhibition of collagen biosynthesis.

Conclusion: Caffeine reduces collagen synthesis in human cultured skin fibroblasts. HA did not have any significant protective effect on this process. This is the first study to our knowledge that reports caffeine-induced inhibition of collagen synthesis in human skin fibroblasts.

Show MeSH