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CSF isoprostane levels are a biomarker of oxidative stress in multiple sclerosis.

Mir F, Lee D, Ray H, Sadiq SA - Neurol Neuroimmunol Neuroinflamm (2014)

Bottom Line: We found increased CSF levels of 8-iso-PGF2α in patients with MS compared to controls, with the most striking values in a subgroup of patients with SPMS.Furthermore, the increase in 8-iso-PGF2α correlated with other parameters of lipid peroxidation as well as with a decrease in the total antioxidant status in the MS CSF samples.Further investigation will help establish the pathologic and clinical significance of our preliminary findings.

View Article: PubMed Central - PubMed

Affiliation: Tisch MS Research Center of New York, New York, NY.

ABSTRACT

Objective: To investigate the potential of 8-iso-prostaglandin F2α (8-iso-PGF2α) as a biomarker for disease activity and oxidative stress in the CSF of patients with multiple sclerosis (MS).

Methods: The isoprostane 8-iso-PGF2α is an established biomarker for in vivo oxidative stress and lipid peroxidation. We measured CSF 8-isoPGF2α levels in 231 patients with MS (74 with relapsing-remitting MS, 67 with primary progressive MS, and 90 with secondary progressive MS [SPMS]) and 40 controls using a competition ELISA.

Results: We found increased CSF levels of 8-iso-PGF2α in patients with MS compared to controls, with the most striking values in a subgroup of patients with SPMS. Furthermore, the increase in 8-iso-PGF2α correlated with other parameters of lipid peroxidation as well as with a decrease in the total antioxidant status in the MS CSF samples.

Conclusions: Our study demonstrates that CSF levels of 8-iso-PGF2α may serve as a biomarker of oxidative stress in MS. Further investigation will help establish the pathologic and clinical significance of our preliminary findings.

No MeSH data available.


Related in: MedlinePlus

Correlation of CSF 8-iso-PGF2α levels with other indicators of oxidative stressCorrelations of 8-iso-prostaglandin F2α (8-iso-PGF2α) values with the corresponding values for superoxide dismutase (SOD), oxidized glutathione (GSH), and thiobarbituric acid reactive substances (TBARS) in the CSF samples of patients with multiple sclerosis are shown as a scatter plot. The corresponding values are given at the bottom.
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Figure 2: Correlation of CSF 8-iso-PGF2α levels with other indicators of oxidative stressCorrelations of 8-iso-prostaglandin F2α (8-iso-PGF2α) values with the corresponding values for superoxide dismutase (SOD), oxidized glutathione (GSH), and thiobarbituric acid reactive substances (TBARS) in the CSF samples of patients with multiple sclerosis are shown as a scatter plot. The corresponding values are given at the bottom.

Mentions: In order to establish oxidative stress, we also measured other parameters of oxidative stress/lipid peroxidation, namely MDA via TBARS, GSSG, and SOD, in the CSF samples of patients with MS. In the 124 samples assayed for all the different parameters, CSF 8-iso-PGF2α levels showed a high correlation with TBARS (r = 0.78) and GSSG (r = 0.63) but did not correlate with SOD (r = 0.016), as shown in figure 2.


CSF isoprostane levels are a biomarker of oxidative stress in multiple sclerosis.

Mir F, Lee D, Ray H, Sadiq SA - Neurol Neuroimmunol Neuroinflamm (2014)

Correlation of CSF 8-iso-PGF2α levels with other indicators of oxidative stressCorrelations of 8-iso-prostaglandin F2α (8-iso-PGF2α) values with the corresponding values for superoxide dismutase (SOD), oxidized glutathione (GSH), and thiobarbituric acid reactive substances (TBARS) in the CSF samples of patients with multiple sclerosis are shown as a scatter plot. The corresponding values are given at the bottom.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4202929&req=5

Figure 2: Correlation of CSF 8-iso-PGF2α levels with other indicators of oxidative stressCorrelations of 8-iso-prostaglandin F2α (8-iso-PGF2α) values with the corresponding values for superoxide dismutase (SOD), oxidized glutathione (GSH), and thiobarbituric acid reactive substances (TBARS) in the CSF samples of patients with multiple sclerosis are shown as a scatter plot. The corresponding values are given at the bottom.
Mentions: In order to establish oxidative stress, we also measured other parameters of oxidative stress/lipid peroxidation, namely MDA via TBARS, GSSG, and SOD, in the CSF samples of patients with MS. In the 124 samples assayed for all the different parameters, CSF 8-iso-PGF2α levels showed a high correlation with TBARS (r = 0.78) and GSSG (r = 0.63) but did not correlate with SOD (r = 0.016), as shown in figure 2.

Bottom Line: We found increased CSF levels of 8-iso-PGF2α in patients with MS compared to controls, with the most striking values in a subgroup of patients with SPMS.Furthermore, the increase in 8-iso-PGF2α correlated with other parameters of lipid peroxidation as well as with a decrease in the total antioxidant status in the MS CSF samples.Further investigation will help establish the pathologic and clinical significance of our preliminary findings.

View Article: PubMed Central - PubMed

Affiliation: Tisch MS Research Center of New York, New York, NY.

ABSTRACT

Objective: To investigate the potential of 8-iso-prostaglandin F2α (8-iso-PGF2α) as a biomarker for disease activity and oxidative stress in the CSF of patients with multiple sclerosis (MS).

Methods: The isoprostane 8-iso-PGF2α is an established biomarker for in vivo oxidative stress and lipid peroxidation. We measured CSF 8-isoPGF2α levels in 231 patients with MS (74 with relapsing-remitting MS, 67 with primary progressive MS, and 90 with secondary progressive MS [SPMS]) and 40 controls using a competition ELISA.

Results: We found increased CSF levels of 8-iso-PGF2α in patients with MS compared to controls, with the most striking values in a subgroup of patients with SPMS. Furthermore, the increase in 8-iso-PGF2α correlated with other parameters of lipid peroxidation as well as with a decrease in the total antioxidant status in the MS CSF samples.

Conclusions: Our study demonstrates that CSF levels of 8-iso-PGF2α may serve as a biomarker of oxidative stress in MS. Further investigation will help establish the pathologic and clinical significance of our preliminary findings.

No MeSH data available.


Related in: MedlinePlus