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Association between genetic variations in GRHL2 and noise-induced hearing loss in Chinese high intensity noise exposed workers: a case-control analysis.

Li X, Huo X, Liu K, Li X, Wang M, Chu H, Hu F, Sheng H, Zhang Z, Zhu B - Ind Health (2013)

Bottom Line: We tested whether GRHL2 polymorphisms are associated with the risk of noise-induced hearing loss (NIHL) in Chinese high intensity noise exposed workers.No altered NIHL risk was associated with the other five polymorphisms.Polymorphisms of GRHL2 may positively contribute to the etiology of NIHL.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Genomics, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, China.

ABSTRACT
The grainyhead like 2 (GRHL2) is a transcription factor, and the role among noise exposed workers is not well established. We tested whether GRHL2 polymorphisms are associated with the risk of noise-induced hearing loss (NIHL) in Chinese high intensity noise exposed workers. We genotyped six polymorphisms of GRHL2 gene (i.e., rs611419, rs3779617, rs3735713, rs3735714, rs3735715, and rs6989650) of 340 NIHL cases and 356 control subjects who exposed to noise higher than 85 dB (A) [Lex, 8 h=time-weighted average of levels of noise exposure (Lex) for a nominal 8 h working day] in a Chinese population. Compared with rs611419 AA genotype, the AT/TT genotypes conferred protection against NIHL [adjusted odds ratio (OR)=0.71, 95% confidence interval (CI)=0.52-0.98]. No altered NIHL risk was associated with the other five polymorphisms. In the combined analyses, we found that the combined genotypes with three to eight variant alleles were associated with an decrease risk of NIHL compared with those with zero to two variant alleles, and the decrease risk was more pronounced among subgroups of exposure time>20 yr (0.31, 0.16-0.62) and drinkers (0.51, 0.29-0.90). Polymorphisms of GRHL2 may positively contribute to the etiology of NIHL.

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Related in: MedlinePlus

Pairwise Linkage Disequilibrium (LD) among seven SNPs in 3′ untranslated region ofthe GRHL2 gene.The LD plots were generated by Haploview 4.1. The value within each diamondrepresents the pairwise correlation (R2) between SNPs defined by the upperleft and the upper right sides of the diamond. The diamonds without value mean theR2=1.0, showed complete linkage disequilibrium.
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fig_001: Pairwise Linkage Disequilibrium (LD) among seven SNPs in 3′ untranslated region ofthe GRHL2 gene.The LD plots were generated by Haploview 4.1. The value within each diamondrepresents the pairwise correlation (R2) between SNPs defined by the upperleft and the upper right sides of the diamond. The diamonds without value mean theR2=1.0, showed complete linkage disequilibrium.

Mentions: Recently, many genome-wide association studies (GWASs) have found that there might besome other functional polymorphisms located downstream or upstream of the genes, even inintergenic regions known as gene deserts. But from a gene perspective, the 5′ near gene(as promoter region), 5′ untranslated region (5′ UTR), 3′ UTR, or coding regions withamino acid changes are the most popular potentially functional regions in currentassociation studies. So we used the SNP selection strategy which only chose the aboveregions. SNPs were selected from the National Center for Biotechnology Information (NCBI)database (http://www.ncbi.nlm.cih.gov/) with a minor allele frequency (MAF)>0.10 in HanChinese. Potentially functional polymorphisms were identified to meet the followingcriteria: located in the 5′ near gene, 5′ UTR, 3′ UTR, or coding regions with amino acidchanges. According to the criteria, nine SNPs were identified, seven of which were locatedin 3′ UTR. We then calculated correlation coefficient (R2) for each pair of theseven SNPs in 3′ UTR and found that rs3824090, rs3824091 and rs6989050 were in completelinkage disequilibrium (LD) (R2=1.00), while rs3735715 and rs3735717 were in LDtoo (Fig. 1Fig. 1.


Association between genetic variations in GRHL2 and noise-induced hearing loss in Chinese high intensity noise exposed workers: a case-control analysis.

Li X, Huo X, Liu K, Li X, Wang M, Chu H, Hu F, Sheng H, Zhang Z, Zhu B - Ind Health (2013)

Pairwise Linkage Disequilibrium (LD) among seven SNPs in 3′ untranslated region ofthe GRHL2 gene.The LD plots were generated by Haploview 4.1. The value within each diamondrepresents the pairwise correlation (R2) between SNPs defined by the upperleft and the upper right sides of the diamond. The diamonds without value mean theR2=1.0, showed complete linkage disequilibrium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4202744&req=5

fig_001: Pairwise Linkage Disequilibrium (LD) among seven SNPs in 3′ untranslated region ofthe GRHL2 gene.The LD plots were generated by Haploview 4.1. The value within each diamondrepresents the pairwise correlation (R2) between SNPs defined by the upperleft and the upper right sides of the diamond. The diamonds without value mean theR2=1.0, showed complete linkage disequilibrium.
Mentions: Recently, many genome-wide association studies (GWASs) have found that there might besome other functional polymorphisms located downstream or upstream of the genes, even inintergenic regions known as gene deserts. But from a gene perspective, the 5′ near gene(as promoter region), 5′ untranslated region (5′ UTR), 3′ UTR, or coding regions withamino acid changes are the most popular potentially functional regions in currentassociation studies. So we used the SNP selection strategy which only chose the aboveregions. SNPs were selected from the National Center for Biotechnology Information (NCBI)database (http://www.ncbi.nlm.cih.gov/) with a minor allele frequency (MAF)>0.10 in HanChinese. Potentially functional polymorphisms were identified to meet the followingcriteria: located in the 5′ near gene, 5′ UTR, 3′ UTR, or coding regions with amino acidchanges. According to the criteria, nine SNPs were identified, seven of which were locatedin 3′ UTR. We then calculated correlation coefficient (R2) for each pair of theseven SNPs in 3′ UTR and found that rs3824090, rs3824091 and rs6989050 were in completelinkage disequilibrium (LD) (R2=1.00), while rs3735715 and rs3735717 were in LDtoo (Fig. 1Fig. 1.

Bottom Line: We tested whether GRHL2 polymorphisms are associated with the risk of noise-induced hearing loss (NIHL) in Chinese high intensity noise exposed workers.No altered NIHL risk was associated with the other five polymorphisms.Polymorphisms of GRHL2 may positively contribute to the etiology of NIHL.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Genomics, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing Medical University, China.

ABSTRACT
The grainyhead like 2 (GRHL2) is a transcription factor, and the role among noise exposed workers is not well established. We tested whether GRHL2 polymorphisms are associated with the risk of noise-induced hearing loss (NIHL) in Chinese high intensity noise exposed workers. We genotyped six polymorphisms of GRHL2 gene (i.e., rs611419, rs3779617, rs3735713, rs3735714, rs3735715, and rs6989650) of 340 NIHL cases and 356 control subjects who exposed to noise higher than 85 dB (A) [Lex, 8 h=time-weighted average of levels of noise exposure (Lex) for a nominal 8 h working day] in a Chinese population. Compared with rs611419 AA genotype, the AT/TT genotypes conferred protection against NIHL [adjusted odds ratio (OR)=0.71, 95% confidence interval (CI)=0.52-0.98]. No altered NIHL risk was associated with the other five polymorphisms. In the combined analyses, we found that the combined genotypes with three to eight variant alleles were associated with an decrease risk of NIHL compared with those with zero to two variant alleles, and the decrease risk was more pronounced among subgroups of exposure time>20 yr (0.31, 0.16-0.62) and drinkers (0.51, 0.29-0.90). Polymorphisms of GRHL2 may positively contribute to the etiology of NIHL.

Show MeSH
Related in: MedlinePlus